講授課程
1.《生物化學》
2.《現代生物化學》
研究方向
1. 單分子分析
2. 腫瘤診療一體化
科研項目
1.
國家自然科學基金面上項目,基於納米孔技術的肺癌相關活性分子檢測及電化學分析機制探索(21874062),65萬元,2019. 1–2022. 12,主持
2. 國家自然科學基金青年項目,基於DNA重組技術檢測腫瘤細胞及細胞內活性物質的研究(21405073),25萬元,2015. 1–2017. 12,主持
3.
山東省科技發展計畫,煤礦瓦斯高靈敏探測攜帶型設備研製(2014GSF120016),12萬元,2014. 7–2015. 12,主持
4. 山東省博士基金,水稻CCCH型鋅指轉錄因子亞家族I在抗逆境脅迫中的功能研究(BS2011SW001),6萬元,2011. 7–2014. 7,主持
5. 國家重點實驗室開放課題,Dof轉錄因子在水稻種子發育中的功能研究(2011KF10),5萬元,2012.6–2014.6,主持
6. 國家重點實驗室開放課題,基於環介導恆溫擴增反應放大技術檢測腫瘤細胞內活性物質的研究(SKLEAC201502),3萬元,2015. 6–2016. 12,主持
7. 青島科技大學博士後人員項目啟動資金,利用無細胞翻譯體系檢測DNA和腫瘤細胞的研究,5萬元,2014. 7–2017. 7,主持
代表性論文
1. Zhen Fang, Liping Liu, Ying Wang, Dongmei Xi*, and Shusheng Zhang*. Unambiguous discrimination of multiple protein biomarkers by nanopore sensing with double-stranded DNA-based probes. Analytical Chemistry, 2020, 92, 1730-1737. SCI一區, TOP期刊. 通訊作者
2. Dongmei Xi, Zhi Li, Liping Liu, Shiyun Ai, Shusheng Zhang*. Ultrasensitive detection of cancer cells combining enzymatic signal amplification with an aerolysin nanopore. Analytical Chemistry, 2018, 90, 1029-1034. SCI一區, TOP期刊. 第一作者
3. Dongmei Xi, Jizhen Shang, Enguo Fan, Jinmao You, Shusheng Zhang*, and Hua Wang*. Nanopore-based selective discrimination of micrornas with single-nucleotide difference using locked nucleic acid-modified probes. Analytical Chemistry, 2016, 88, 10540-10546. SCI一區, TOP期刊. 第一作者
4. Jizhen Shang, Zhi Li, Liping Liu, Dongmei Xi*, and Hua Wang*. Label-free sensing of human 8-oxoguanine DNA glycosylase activity with a nanopore. ACS Sensors, 2018, 3, 512-518. SCI一區, TOP期刊. 通訊作者
5.Liping Liu, Zhen Fang, Xiangjiang Zheng, and Dongmei Xi*. Nanopore-based strategy for sensing of copper (II) ion and real-time monitoring of a click reaction. ACS Sensors, 2019, 4, 1323-1328. SCI一區, TOP期刊. 通訊作者
6. Dongmei Xi, Xindong Wang, Shiyun Ai and Shusheng Zhang*. Detection of cancer cells using triplex DNA molecular beacons based on expression of enhanced green fluorescent protein (eGFP). Chemical Communications, 2014, 50, 9547-9549. SCI一區, TOP期刊. 第一作者
7. Ying Wang*, Binxiao Li, Xiangdong Li, Dongmei Xi*. Fluorescent detection of zucchini yellow mosaic virus based on recombinase polymerase amplification and enzyme-assisted signal amplification. Microchemical Journal, 2020, 159, 105384. SCI二區. 通訊作者
8. Xiangjiang Zheng*, Jiayu Zhang, Zhaomei Sun, Zhen Zhang, and Dongmei Xi*. Fabrication of amorphous Cu–Co–P nanofilms on CuCo2C4 nanoarrays by in situ electrochemical reduction for efficient hydrogen evolution in alkaline solution. European Journal of Inorganic Chemistry, 2018, 3565-3569. SCI二區. 通訊作者
9. 李志, 劉麗萍, 房真,郗冬梅*. 固體納米孔在生物感測中的套用研究進展, 分析化學, 2018, 6, 836-842. SCI四區. 通訊作者
10. Dongmei Xi, Wushuang Liu, Guodong Yang, Changai Wu and Chengchao Zheng*. Seed-specific overexpression of antioxidant genes in Arabidopsis enhances oxidative stress tolerance during germination and early seedling growth. Plant Biotechnology Journal, 2010, 8(7): 796-806. SCI一區. 第一作者
11. Dongmei Xi, Chengchao Zheng*. Transcriptional regulation of seed storage protein genes in Arabidopsis and cereals. Seed Science Research, 2011, 21(4): 247-254. SCI三區. 第一作者
12. Liang Zhang, Dongmei Xi, Shanwei Li, Zheng Gao, Shuoli Zhao, Jing Shi, Changai Wu, Xingqi Guo*. A cotton group C MAP kinase gene, GhMPK2, positively regulates salt and drought tolerance in tobacco. Plant Molecular Biology, 2011, 77(1-2): 17-31. SCI二區. 並列第一作者
13. Zhaohua Liu, Dongmei Xi, Mingjiang Kang, Xingqi Guo*, Baohua Xu*. Molecular cloning and characterization of Hsp27.6: the first reported small heat shock protein from Apis cerana cerana. Cell Stress & Chaperones, 2012, 17(5): 539-551. SCI三區. 並列第一作者
出版圖書
1. 專著, Nucleic Acid Amplification Strategies for Biosensing, Bioimaging and Biomedicine (專章Chapter 9), Springer出版社, 2019. 4, 專章第一位
2. 教材, 生物化學精要, 科學出版社, 2017. 4, 編委
發明專利
1. 一種利用α-溶血素納米孔檢測DNA糖基化酶活性的方法, 國家發明專利, 專利號ZL201810050228.X. 第一位. 2019.4授權
2. 檢測KRAS基因突變的LNA探針及檢測方法, 國家發明專利, 申請號CN201610902490.3. 第一位. 2016.10
3. 一種利用α-溶血素納米孔檢測銅離子的方法, 國家發明專利,申請號201910365826.0. 第一位. 2019.5
獲獎記錄
1. 山東高等學校優秀科研成果二等獎,基於光電感測技術的腫瘤標誌物檢測新方法研究,第二位,2015
2. 臨沂大學青年教學能手,2012
3. 山東省大學生生物化學實驗技能大賽優秀指導教師,2013