基本介紹
- 中文名：蔡孟浩
- 國籍：中國
- 民族：漢
- 出生地：山東
- 畢業院校：華東理工大學
- 學位/學歷：博士/研究生
- 專業方向：生物工程/生物化工
華東理工大學理工優秀生部生物工程本科，英國布里斯托大學化學院聯合培養博士生，華東理工大學生物化工博士學位。擔任中國微生物學會海洋微生物學專業委員會委員，中國生物化學與分子生物學學會海洋生物化學與分子生物學分會理事會理事。研究工作發表在J Biol Chem、Microb Cell Fact、J Biotechnol、Bioresourc Technol、Mar Biotechnol等生物技術權威期刊。申請中國發明專利12項，已授權6項。擔任Microb Cell Fact、Appl Microbiol Biotechnol、Appl Biochem Biotechnol、Biotechnol Appl Biochem、Biochem Eng J等期刊審稿人。
發表論文（*通訊作者；#共同第一作者）：
1.Jinjia Wang, Xiaolong Wang, Lei Shi, Fei Qi, Ping Zhang, Yuanxing Zhang, Xiangshan Zhou*, Zhiwei Song*, Menghao Cai*. Methanol-independent protein expression by AOX1 promoter with trans-acting elements engineering and glucose-glycerol-shift induction in Pichia pastoris. Scientific Reports 2017, 7: 41850.
2.Ying Xue, Chuixing Kong, Wei Shen, Chenxiao Bai, Yanna Ren, Xiangshan Zhou, Yuanxing Zhang, Menghao Cai*. Methylotrophic yeast Pichia pastoris as a chassis organism for polyketide synthesis via the full citrinin biosynthetic pathway. Journal of Biotechnology. 2017, 242:64-72
3.Yanyun Gao, Mengmeng Huang, Xiaoyue Sun, Xiaoxu Zhang, Yuanxing Zhang, Xiangshan Zhou*, Menghao Cai*. Single-site mutation of C363G or N463T strengthens thermostability improvement of IG181-182 deleted acidic α-amylase from deep-sea thermophile Geobacillus sp. Food Biotechnology. accepted.
4.Xiaolong Wang, Qi Wang, Jinjia Wang, Peng Bai, Lei Shi, Wei Shen, Mian Zhou, Xiangshan Zhou, Yuanxing Zhang, Menghao Cai*. Mit1 transcription factor mediates methanol signaling and regulates alcohol oxidase 1 promoter in Pichia pastoris. Journal of Biological Chemistry. 2016, 291:6245-6261
5.Xiaoxu Zhang, Hao He, Ying Yin, Weiqiang Zhou, Menghao Cai*, Xiangshan Zhou, Yuanxing Zhang. A light–dark shift strategy derived from light-responded metabolic behaviors for polyketides production in marine fungus Halorosellinia sp.. Journal of Biotechnology. 2016, 221:34-42
6.Hao He, Ying Xue, Ning Xu, Ying Yin, Jian Lu, Menghao Cai*, Yuanxing Zhang. A novel pH–precursor–glucose synergic control strategy for dinactin biosynthesis by an arctic actinomycete Streptomyces sp. Process Biochemistry. 2016, 2016, 51:1911-1918.
7.Mengmeng Huang, Yanyun Gao, Xiangshan Zhou, Yuanxing Zhang, Menghao Cai*. Regulating unfolded protein response activator HAC1p for production of thermostable raw-starch hydrolyzing α-amylase in Pichia pastoris. Bioprocess and Biosystem Engineering. 2016, accepted. DOI:10.1007/s00449-016-1701-y
8.Xiaolong Wang, Menghao Cai*, Lei Shi, Qi Wang, Jinxiang Zhu, Jinjia Wang, Mian Zhou, Xiangshan Zhou, Yuanxing Zhang. PpNrg1 is a transcriptional repressor for glucose and glycerol repression of AOX1 promoter in methylotrophic yeast Pichia pastoris. Biotechnology Letters. 2016, 38:291-298.
9.Chu Shen, Ning Xu, Yanyun Gao, Xiaoyue Sun, Ying Yin*, Menghao Cai*, Xiangshan Zhou, Yuanxing Zhang. Stimulatory effect of ethanol on libertellenone H biosynthesis by Arctic fungus Eutypella sp. D-1. Bioprocess and Biosystem Engineering. 2016, 39:353-360
10.Tao Jiang, Menghao Cai*, Mengmeng Huang, Hao He, Jian Lu, Xiangshan Zhou, Yuanxing Zhang. Bioprocess exploration for thermostable α-amylase production of a deep-sea thermophile Geobacillus sp. in high temperature bioreactor. Preparative Biochemistry & Biotechnology. 2016, 17, 46:620-627
11.Siwei Xiao, Yanyun Gao, Xiaolong Wang, Wei Shen, Jinjia Wang, Xiangshan Zhou, Menghao Cai*, Yuanxing Zhang. Peroxisome-targeted and tandem repeat multimer expressions of human antimicrobial peptide LL37 in Pichia pastoris. Preparative Biochemistry & Biotechnology. 2016, 27:1-7
12.Shaomei Liu, Jiaxin Li, Yuan Wu, Yanna Ren, Qi Liu, Qiyao Wang, Xiangshan Zhou, Menghao Cai*, Yuanxing Zhang. De novo transcriptome sequencing of marine-derived Aspergillus glaucus and comparative analysis of metabolic and developmental variations in response to salt stress. Genes & Genomics. 2016, accepted. DOI:10.1007/s13258-016-0497-0
13.Meixia Wang, Hao He, Ke Na, Menghao Cai*, Xiangshan Zhou, Wenjie Zhao, Yuanxing Zhang. Designing novel glucose/ornithine replenishment strategies by biosynthetic and bioprocess analysis to improve fibrinolytic FGFC1 production by the marine fungus Stachybotrys longispora. Process Biochemistry. 2015, 50:2012-2018
14.Tao Jiang, Menghao Cai*, Mengmeng Huang, Hao He, Jian Lu, Xiangshan Zhou, Yuanxing Zhang. Characterization of a thermostable raw-starch hydrolyzing a-amylase from deep-sea thermophile Geobacillus sp. Protein Expression and Purification. 2015, 114:15-22
15.Jiao Zhou, Hao He, Xiaolong Wang, Jian Lu, Xiangshan Zhou, Menghao Cai*, Yuanxing Zhang. Optimization of nutrients for dinactin production by a marine Streptomyces sp. from the High Latitude Arctic. Biotechnology Bioprocess Engineering. 2015, 20:725-732
16.胡偉，李佳欣，李曉霞，蔡孟浩*，周祥山*，張元興. 剪下敏感型海洋灰綠麴黴AgugtA和AgugeA基因的克隆及功能分析. 華東理工大學學報（自然科學版）. 2015, 41: 455-463.
17.Menghao Cai#, Ying Zhang#, Wei Hu, Wei Shen, Zhenzhong Yu, Weiqiang Zhou, Tao Jiang, Xiangshan Zhou *, Yuanxing Zhang.. Genetically shaping morphology of the filamentous fungus Aspergillus glaucus for production of antitumor polyketide aspergiolide A. Microbial Cell Factories. 2014, 13:73
18.Weiqiang Zhou, Menghao Cai*, Ke Na, Chu Shen, Xiaoxu Zhang, Xiangshan Zhou, Wenjie Zhao, Yuanxing Zhang. pH-dependent accumulation of anticancer compound on mycelia in fermentation of marine fungus. Journal of Industrial Microbiology & Biotechnology. 2014, 41: 1169-1173
19.Zhenzhong Yu, Menghao Cai*, Wei Hu, Ying Zhang, Jiao Zhou, Xiangshan Zhou*, Yuanxing Zhang. A cyclin-like protein, ClgA, regulates development in Aspergillus nidulans. Research in Microbiology. 2014, 165(6): 462-467
20.Jiushun Zhou, Menghao Cai*, Tao Jiang, Weiqiang Zhou, Wei Shen, Xiangshan Zhou, Yuanxing Zhang. Mixed carbon source control strategy for enhancing alginate lyase production by marine Vibrio sp. QY102. Bioprocess and Biosystem Engineering. 2014, 37:575-584
21.Limei Gao, Menghao Cai*, Wei Shen, Siwei Xiao, Xiangshan Zhou and Yuanxing Zhang. Engineered fungal polyketide biosynthesis in Pichia pastoris: a potential excellent host for polyketide production.Microbial Cell Factories 2013, 12:77
22.Weiqiang Zhou, Menghao Cai*, Jiushun Zhou, Tao Jiang, Jiao Zhou, Meixia Wang, Xiangshan Zhou*, Yuanxing Zhang. Nutrition and bioprocess development for efficient biosynthesis of an antitumor compound from marine-derived fungus.Journal of Industrial Microbiology & Biotechnology. 2013, 40:1131-1142.
23.Jack Davison, Ahmed al Fahad, Menghao Cai, Zhongshu Song, Samar Y. Yehia, Colin M. Lazarus, Andrew M. Bailey, Thomas J. Simpson, Russell J. Cox*. Genetic, molecular, and biochemical basis of fungal tropolone biosynthesis.Proceedings of the National Academy of Sciences of the United States of America (PNAS). 2012, 109: 7642-7647.
24.Menghao Cai, Zhe Fang, Chuanpeng Niu, Xiangshan Zhou*, Yuanxing Zhang. Light regulation on growth, development and secondary metabolism of marine-derived filamentous fungi.Folia Microbiologica. 2013, 58: 537-546.
25.Menghao Cai, Xiangshan Zhou*, Jian Lu, Weimin Fan, Jiushun Zhou, Chuanpeng Niu, Li Kang, Xueqian Sun, Yuanxing Zhang*. An integrated control strategy for the fermentation of the marine-derived fungus Aspergillus glaucus for the production of anti-cancer polyketide. Marine Biotechnology 2012, 14(6): 665-671.
26.Menghao Cai, Xueqian Sun, Xiangshan Zhou*, Yuanxing Zhang*. Roles of cobalt in biosynthesis stimulation of a cytotoxic compound from marine-derivedAspergillus glaucus. Process Biochemistry. 2012, 12: 2267–2274.
27.Menghao Cai, Xiangshan Zhou*, Jian Lu, Weimin Fan, Chuanpeng Niu, Jiushun Zhou, Xueqian Sun, Li Kang and Yuanxing Zhang*. Enhancing aspergiolide A production from a shear-sensitive and easy-foaming marine-derived filamentous fungus Aspergillus glaucus by oxygen carrier addition and impeller combination in a bioreactor. Bioresource Technology 2011, 102: 3584-3586.
28.Menghao Cai, Xiangshan Zhou*, Jiushun Zhou, Chuanpeng Niu, Xueqian Sun and Yuanxing Zhang*. Efficient strategy for enhancing aspergiolide A production by citrate feedings and its effects on sexual development and growth of marine-derived fungus Aspergillus glaucus. Bioresource Technology 2010, 101: 6059-6068.
29.Meng-Hao Cai, Xiang-Shan Zhou*, Xue-Qian Sun, Ke-Jing Tao and Yuan-Xing Zhang*. Statistical optimization of medium composition for aspergiolide A production by marine-derived fungus Aspergillus glaucus. Journal of Industrial Microbiology & Biotechnology 2009, 36: 381-389.
30.Chuanpeng Niu, Menghao Cai, Yuanxing Zhang, Xiangshan Zhou*. Biosynthetic origin of the carbon skeleton of a novel anti-tumor compound, haloroquinone, from a marine-derived fungus, Halorosellinia sp. Biotechnology Letters. 2012, 34:2119-2124
31.Li Kang, Menghao Cai, Chao Yu, Yuanxing Zhang, Xiangshan Zhou*. Improved production of the anticancer compound 1403C by glucose pulse feeding of marine Halorosellinia sp. (No. 1403) in submerged culture Bioresource Technology 2011, 102:10750-10753.
32.Chao Yu, Menghao Cai, Li Kang, Yuanxing Zhang, Xiangshan Zhou*. Significance of seed culture methods on mycelial morphology and production of a novel anti-cancer anthraquinone by marine mangrove endophytic fungus Halorosellinia sp. (No. 1403). Process Biochemistry 2012, 47:422-427.
33.Zhe Fang, Ying Zhang, Menghao Cai, Jian Zhang,Yuanxing Zhang, Xiangshan Zhou*. Improved gene targeting frequency in marine-derived filamentous fungus Aspergillus glaucus by disrupting ligD. Journal of Applied Genetics 2012, 53:355–362
34.Pham Ngoc Tu, Yamin Wang, Menghao Cai, Xiangshan Zhou*, Yuanxing Zhang*. Co-expressions of protein tyrosine kinases EGFR-2 and PDGFRβ with protein tyrosine phosphatase 1B in Pichia pastoris. Journal of Microbiology and Biotechnology 2014, 24: 152-159
35.Sanying Wang, Qin Liu*, Menghao Cai, Qiyao Wang, Yuanxing Zhang. Fermentation preparation of recombinant Vibrio anguillarum vaccine with heterogeneous antigen display. Preparative Biochemistry and Biotechnology. 2013, 43:79-94.
36.Xueqian Sun, Xiangshan Zhou*, Menghao Cai, Kejing Tao, Yuanxing Zhang*. Identified biosynthetic pathway of aspergiolide A and a novel strategy to increase its production in a marine-derived fungus Aspergillus glaucus by feeding of biosynthetic precursors and inhibitors simultaneously. Bioresource Technology 2009, 100: 4244-4251.
37.Xueqian Sun, Xiangshan Zhou*, Menghao Cai, Jiushun Zhou, Yuanxing Zhang*. Significant stimulation of o-phthalic acid in biosynthesis of aspergiolide A by a marine fungus Aspergillus glaucus. Bioresource Technology 2010, 101: 3609-3616.
38.Kejing Tao, Lin Du, Xueqian Sun, Menghao Cai, Tianjiao Zhu, Xiangshan Zhou*, Qianqun Gu, Yuanxing Zhang. Biosynthesis of aspergiolide A, a novel antitumor compound by a marine-derived fungus Aspergillus glaucus via the polyketide pathway. Tetrahedron Letters 2009, 50: 1082-1085.
39.李曉霞, 蔡孟浩*, 胡偉, 李佳欣, 周祥山*, 張元興. 海洋灰綠麴黴聚酮合酶基因的克隆與序列分析. 華東理工大學學報（自然科學版）. 2014, 40: 579-585.
40.亓飛, 蔡孟浩, 周祥山*, 張元興. 基於rna-seq技術的畢赤酵母基因組重注釋及新型強啟動子的發現與序列分析. 華東理工大學學報（自然科學版）. 2014, 40:167-175.
41.柏鵬, 蔡孟浩, 周祥山*, 張元興. 葡萄糖誘導型AOX1啟動子畢赤酵母突變株B5的篩選. 工業微生物2013, 43: 69-72.
42.牛傳朋, 蔡孟浩, 李曉霞, 周祥山*, 張元興. 海洋真菌Xylaria sp.(No.2508)生產xyloketal B的發酵最佳化.工業微生物 2012, 42:43-47.
43.張盈, 沈偉, 蔡孟浩, 周祥山*, 張元興. 海洋真菌灰綠麴黴地標蛋白編碼基因AgTeaA的克隆與序列分析. 生物技術通報 2011, 12: 157-161.
44.康麗, 蔡孟浩, 范衛民, 陸健, 周祥山*, 張元興. 海洋紅樹林內生真菌產抗腫瘤活性化合物1403C發酵條件的最佳化. 生物技術通報 2011, 12: 199-204.
專利：
1.蔡孟浩, 劉一奇, 張元興. 生產洛伐他汀及莫納可林 J 的基因工程菌的構建與套用. 中國發明專利. 申請號201510552643.1
2.蔡孟浩, 周久順, 周祥山, 張元興. 一種利用海洋弧菌生產褐藻膠裂解酶的方法. 中國發明專利. 專利號ZL201210068851.0
3.蔡孟浩, 王美霞, 周祥山, 張元興. 用於海洋長孢葡萄穗霉生產纖溶活性化合物的培養基. 中國發明專利.專利號ZL201310170435.6
4.蔡孟浩, 周嬌, 周祥山, 張元興. 用於北極海洋放線菌Streptomyces sp.生產大環四重內酯類化合物的培養基. 中國發明專利. 申請號201310504941.4
5.蔡孟浩, 沈處, 周祥山, 張元興. 套用於北極絲狀真菌生產二萜類化合物的發酵培養基. 中國發明專利. 申請號201510408258.X
6.蔡孟浩, 沈處, 周祥山, 張元興. 添加醇類促進二萜類化合物Libertellenone H產量的方法. 中國發明專利. 申請號201510408062.0
7.張元興, 蔡孟浩, 周祥山, 蔣濤, 黃猛猛. 一種耐高溫澱粉酶、其編碼基因及其套用. 中國發明專利. 申請號201410712820.3
8.周祥山, 蔡孟浩, 張元興, 孫學謙, 陶可敬, 范衛民, 陸健, 顧謙群, 朱天驕. 一種用於海洋灰綠麴黴生產新型抗腫瘤活性化合物的新型培養基及其製備方法. 中國發明專利, 專利號ZL200810038598.8
9.周祥山, 周衛強, 蔡孟浩, 張元興.利用海洋紅樹林內生真菌生產抗癌蒽醌類化合物的培養基及製備方法，中國發明專利, 專利號ZL201210067014.6
10.周祥山, 蔣濤, 蔡孟浩, 張元興.用於深海海洋嗜熱細菌Geobacillus sp.生產澱粉酶的培養基. 中國發明專利, 專利號ZL201310169378.X
11.周祥山, 蔣濤, 蔡孟浩, 張元興.用於高密度培養深海海洋嗜熱細菌Geobacillus sp.的培養基. 中國發明專利,專利號ZL201310170443.0
12.周祥山, 周衛強. 蔡孟浩. 張元興一種發酵培養海洋真菌生產抗癌化合物1403C的方法.中國發明專利. 申請號201310269533.5
