《致齲變形鏈球菌全基因組的蛋白表達及三維結構研究》是蘇曉東為項目負責人，北京大學為依託單位的青年科學基金項目。
基本介紹
- 中文名：致齲變形鏈球菌全基因組的蛋白表達及三維結構研究
- 項目類別 ：重點項目
- 依託單位 ：北京大學
- 項目負責人：蘇曉東
科研成果,項目摘要,
科研成果
序號 | 標題 | 類型 | 作者 |
|---|---|---|---|
1 | An automated image-collection system for crystallization experiments using SBS standard microplates | 期刊論文 | Su, Xiao-Dong|Nan, Jie|Brostromer, Erik| |
2 | Crystal Structures of Phosphotransferase System Enzymes PtxB (IIB(Asc)) and PtxA (IIA(Asc)) from Streptococcus mutans | 期刊論文 | Lei J|Su XD|Li LF| |
3 | Ring-opening mechanism revealed by crystal structures of NagB and its ES intermediate complex | 期刊論文 | Liang, Yu-He|Liu, Cong|Li, Lan-Fen|Su, Xiao-Dong|Li, Dan| |
4 | Preparation, crystallization and preliminary X-ray analysis of the methionine synthase (MetE) from Streptococcus mutans | 期刊論文 | Su, Xiao-Dong|Fu, Tian-Min|Li, Lan-Fen|Zhang, Xiao-Yan|Liang, Yu-He| |
5 | Preliminary X-ray crystallographic analysis of SMU.573, a putative sugar kinase from Streptococcus mutans | 期刊論文 | Yang, Cheng|Zhou, Yan-Feng|Liang, Yu-He|Li, Lan-Fen|Su, Xiao-Dong| |
6 | Protein preparation, crystallization and preliminary X-ray crystallographic analysis of N-acetylglutamate kinase from Streptococcus mutans | 期刊論文 | Zhao, Xiaojun|Liu, Xiang|Gao, Xiong-Zhuo|Liang, Yu-He|Wu, Chang-Wei|Su, Xiao-Dong|Lei, Jian|Li, Lan-Fen| |
7 | Preliminary X-ray crystallographic analysis of a nitric oxide-inducible lactate dehydrogenase from Staphylococcus aureus | 期刊論文 | Dong JB|Li LF|Wu S|Su XD|Liu X| |
8 | 蛋白質晶體學技術的發展與展望 | 期刊論文 | 蘇曉東|南潔|李蘭芬| |
9 | Structure of a fatty-acid-binding protein from Bacillus subtilis determined by sulfur-SAD phasing using in-house chromium radiation | 期刊論文 | Kristensen O|Zhou Y|Brostromer E|Su XD|Nan J|Yang C| |
10 | Protein preparation, crystallization and preliminary X-ray analysis of the C-terminal domain of human RSK1 serine/threonine protein kinase | 期刊論文 | Li, Dan|Nan, Jie|Li, Lanfen|Fu, Tian-Min|Xue, Yafeng|Su, Xiao-Dong| |
11 | Crystallization and preliminary X-ray analysis of three dUTPases from Gram-positive bacteria | 期刊論文 | Li GL|Su XD|Wang J|Li LF| |
12 | Expression, purification, crystallization and preliminary x-ray studies of a deoxycytidylate deaminase from Streptococcus mutans | 期刊論文 | Su Xiao-Dong|Liu Peng|Xian Ding-Chang|Liang Yu-He|Xu Jian-Hua|Li Li-Qin|Hou Hai-Feng|Li Lan-Fen|Dong Yu-Hui|Gao Zeng-Qiang|Xu Rui| |
13 | Crystal structures of Streptococcus mutans 2 '-deoxycytidylate deaminase and its complex with substrate analog and allosteric regulator dCTP center dot Mg2+ | 期刊論文 | Su, Xiao-Dong|Hou, Hai-Feng|Liang, Yu-He|Li, Lan-Fen|Dong, Yu-Hui| |
14 | Preliminary X-ray crystallographic studies of Bacillus subtilis SpeA protein | 期刊論文 | Jian Lei|Lanfen Li|Xiang Liu|Xiao-Yan Liu|Xiao-Dong Su| |
15 | Protein preparation, crystallization and preliminary X-ray crystallographic analysis of SMU.961 protein from the caries pathogen Streptococcus mutans | 期刊論文 | Li, Lan-Fen|Liang, Yu-He|Zhao, XiaoJun|Su, Xiao-Dong|Gao, Xiong-Zhuo| |
16 | Protein preparation, crystallization and preliminary X-ray crystallographic analysis of Smu_195c from caries pathogen Streptococcus mutans | 期刊論文 | Hou Hai-Feng|Gao Zeng-Qiang|Li Li-Qin|Li Lan-Fen|Rui, Xu|Su Xiao-Dong|Liang Yu-He|Dong Yu-Hui| |
17 | Crystallization and preliminary crystallographic analysis of D-alanine-D-alanine ligase from Streptococcus mutans | 期刊論文 | Liang, Yu-He|Tang, De-Wei|Su, Xiao-Dong|Sheng, Yu|Zhao, Xiaojun|Lu, Yong-Zhi|Liu, Xiang-Yu|Li, Lan-Fen| |
18 | Open-closed conformational change revealed by the crystal structures of 3-keto-L-gulonate 6-phosphate decarboxylase from Streptococcus mutans | 期刊論文 | Li LF|Su XD|Liu X|Li GL|Brostromer E|Nan J| |
19 | Protein preparation and preliminary X-ray crystallographic analysis of a putative glucosamine 6-phosphate deaminase from Streptococcus mutants | 期刊論文 | Liang, Yu-He|Liu, Cong|Li, Dan|Hu, Guan-Jing|Li, Lan-Fen|Wei, Shi-Cheng|Su, Xiao-Dong| |
20 | Crystallization and preliminary x-ray crystallographic studies of SMU.134 protein from caries pathogen Streptococcus mutans | 期刊論文 | Li, Hua|Li, Lan-Fen|Zhao, Xiaojun|Su, Xiao-Dong|Liang, Yu-He| |
21 | Preliminary crystallographic studies of purine nucleoside phosphorylase from the cariogenic pathogen Streptococcus mutans | 期刊論文 | Li LF|Su XD|Liu X|Hou QM|Brostromer E| |
22 | Bioinformatics and structural characterization of a hypothetical protein from Streptococcus mutans: implication of antibiotic resistance | 期刊論文 | Liu XY|Kristensen O|Su XD|Brostromer E|Nan J| |
23 | Protein preparation, crystallization and preliminary X-ray crystallographic studies of Smu.1392c from Streptococcus mutans | 期刊論文 | Dong, Yu-Hui|Hou, Hai-Feng|Liang, Yu-He|Su, Xiao-Dong|Li, Lan-Fen|Gao, Zeng-Qiang| |
24 | A large-scale, high-efficiency and low-cost platform for structural genomics studies | 期刊論文 | Xian, Dingchang|Brostromer, Erik|Dong, Yuhui|Su, Xiao-Dong|Nan, Jie|Liu, Peng|Liang, Yuhe|Li, Lanfen| |
25 | 致齲變異鏈球菌 N-乙醯谷氨酸激酶的表達、純化和生化特性研究 | 期刊論文 | 李蘭芬|高雄卓|蘇曉東|趙曉軍|吳常偉| |
26 | Crystallization and preliminary X-ray crystallographic analysis of SMU .412c protein from the caries pathogen Streptococcus mutans | 期刊論文 | Hou QM|Ye ZY|Li LF|Su XD| |
27 | Preparation, crystallization and preliminary X-ray analysis of threonine synthase from Streptococcus mutans | 期刊論文 | Liu XY|Su XD|Tang DW|Li LF|Zhao X|Liang YH|Yu YM| |
28 | Preparation, crystallization and preliminary X-ray crystallographic analysis of Smu.776 from caries pathogen Streptococcus mutans | 期刊論文 | Wang Kai-Tuo|Zhang Xiao-Yan|Su Xiao-Dong|Li Lan-Fen|Wei Shi-Cheng|Liang Yu-He| |
項目摘要
齲齒病是累及全人類的細菌感染性疾病，WHO已將其列入危害人類健康的三大疾病僅次於心腦血管病和癌症，我國衛生部和國家教委已把齲齒列為2000年學生重點防治疾病之一。大量研究證明變形鏈球菌是最主要的致齲細菌，對其進行大規模蛋白結構研究將深化對變鏈菌致齲分子機理的理解，有助於尋找齲病防治的有效靶標以及可作疫苗的抗原蛋白。經多年努力建成的北京大學結構基因組學平台可進行每年1000個以上基因克隆及表達，能夠勝任變鏈菌的全基因組蛋白結構研究，並在2004年進行了200多個變鏈菌基因的先導研究。在此基礎上，擬進行（1）變鏈菌全基因組非膜蛋白基因約1500個的大規模質粒構建；（2）高通量表達、純化可溶性蛋白；（3）培養蛋白晶體，對衍射好的晶體進行結構解析；（4）在結構基礎上，對未知功能的蛋白進行深入分析，由結構信息推動功能研究
