《禽流感滅活病毒抗原高效誘導鼻腔免疫應答機制的研究》是楊倩為項目負責人，南京農業大學為依託單位的面上項目。
基本介紹
- 中文名：禽流感滅活病毒抗原高效誘導鼻腔免疫應答機制的研究
- 項目類別 ：面上項目
- 項目負責人：楊倩
- 依託單位 ：南京農業大學
科研成果,項目摘要,
科研成果
序號 | 標題 | 類型 | 作者 |
|---|---|---|---|
1 | Characteristics of nasal-associated lymphoid tissue (NALT) and nasal absorption capacity in chicken. | 期刊論文 | Kang, Haihong|Yan, Mengfei|Yu, Qinghua|Yang, Qian| |
2 | Effects of different CpG oligodeoxynucleotides with inactivated avian H5N1 influenza virus on mucosal immunity of chickens. | 期刊論文 | Kang, Haihong|Lin, Jian|Yu, Qinghua|Yang, Qian| |
3 | Chicken bone marrow-derived dendritic cells maturation in response to infectious bursal disease virus. | 期刊論文 | Liang, Jinfeng|Yin, Yinyan|Qin, Tao|Yang, Qian| |
4 | The stimulatory effect of different CpG oligonucleotides on the maturation of chicken bone marrow-derived dendritic cells. | 期刊論文 | Kang, Haihong|Lin, Jian|Yu, Qinghua|Yang, Qian| |
5 | Comparison of 3 kinds of Toll-like receptor ligands for inactivated avian H5N1 influenza virus intranasal immunization in chicken. | 期刊論文 | Kang, Haihong|Lin, Jian|Yu, Qinghua|Yang, Qian| |
6 | Whole inactivated avian Influenza H9N2 viruses induce nasal submucosal dendritic cells to sample luminal viruses via transepithelial dendrites and trigger subsequent DC maturation. | 期刊論文 | Liu, Haofei|Lin, Jian|Yu, Qinghua|Yang, Qian| |
7 | The stimulatory effect of TLRs ligands on maturation of chicken bone marrow-derived dendritic cells | 期刊論文 | Kang, Haihong|Lin, Jian|Yu, Qinghua|Yang, Qian| |
8 | Comparison of 3 kinds of Toll-like receptor ligands for inactivated avian H5N1 influenza virus intranasal immunization in chicken | 期刊論文 | Kang, Haihong|Lin, Jian|Yu, Qinghua|Yang, Qian| |
9 | A novel combined adjuvant strongly enhances mucosal and systemic immunity to low pathogenic avian influenza after oral immunization in ducks. | 期刊論文 | Kang, Haihong|Wang, Hongli|Yu, Qinghua|Yang, Qian| |
10 | 雞骨髓源樹突狀細胞體外誘導培養及鑑定 | 期刊論文 | 付佳|梁金逢|陰銀燕|楊倩| |
11 | Enhanced immune response of BMDCs pulsed with H9N2 AIV and CpG. | 期刊論文 | Qin, Tao|Zhu, Li Q|Yu, Qing H|Yang, Qian| |
12 | Bursopentin (BP5) from chicken bursa of fabricius attenuates the immune function of dendritic cells. | 期刊論文 | Yin, Yinyan|Qin, Tao|Yu, Qinghua|Yang, Qian| |
13 | CpG DNA assists the whole inactivated H9N2 influenza virus in crossing the intestinal epithelial barriers via transepithelial uptake of dendritic cell dendrites. | 期刊論文 | Wang, X|Lin, J|Yu, Q|Yang, Q| |
14 | Characterization of nasal cavity-associated lymphoid tissue in ducks. | 期刊論文 | Kang, Haihong|Yan, Mengfei|Yu, Qinghua|Yang, Qian| |
15 | Effects of different CpG oligodeoxynucleotides with inactivated avian H5N1 influenza virus on mucosal immunity of chickens | 期刊論文 | Fu, Jia|Liang, Jinfeng|Kang, Haihong|Lin, Jian|Yu, Qinghua|Yang, Qian| |
16 | The stimulatory effect of TLRs ligands on maturation of chicken bone marrow-derived dendritic cells | 期刊論文 | Kang, Haihong|Lin, Jian|Yu, Qinghua|Yang, Qian| |
17 | CpG oligonucleotides and Astragalus polysaccharides are effective adjuvants in cultures of avian bone marrow-derived dendritic cells. | 期刊論文 | Liang, J|Fu, J|Yu, Q|Yang, Q| |
18 | Bursopentin (BP5) protects dendritic cells from lipopolysaccharide-induced oxidative stress for immunosuppression. | 期刊論文 | Qin, Tao|Yin, Yinyan|Yu, Qinghua|Yang, Qian| |
19 | Characterization of Nasal Cavity-Associated Lymphoid Tissue in Ducks | 期刊論文 | Kang, Haihong|Yan, Mengfei|Yu, Qinghua|Yang, Qian| |
20 | Characteristics of Nasal-Associated Lymphoid Tissue (NALT) and Nasal Absorption Capacity in Chicken | 期刊論文 | Kang, Haihong|Yan, Mengfei|Yu, Qinghua|Yang, Qian| |
21 | H9N2 Influenza Whole Inactivated Virus Combined with Polyethyleneimine Strongly Enhance Mucosal and Systemic Immunity after Intranasal Immunization in Mice. | 期刊論文 | Yin, Yinyan|Huang, Lulu|Yu, Qinghua|Yang, Qian| |
22 | CpG Oligodeoxynucleotides Facilitate Delivery of Whole Inactivated H9N2 Influenza Virus via Transepithelial Dendrites of Dendritic Cells in Nasal Mucosa. | 期刊論文 | Huang, Lulu|Wang, Xiaoqing|Lin, Jian|Yang, Qian| |
23 | The stimulatory effect of different CpG oligonucleotides on the maturation of chicken bone marrow-derived dendritic cells | 期刊論文 | Kang, Haihong|Lin, Jian|Yu, Qinghua|Yang, Qian| |
24 | 雞鼻腔組織學特點及鼻相關淋巴組織分布的研究 | 期刊論文 | 閆夢菲|康海泓|楊倩| |
項目摘要
鼻腔是禽流感病毒入侵動物的主要途徑，鼻腔免疫後可阻斷禽流感病毒進入機體。鼻腔黏膜對滅活禽流感病毒抗原的吸收和樹突狀細胞有效攝取抗原是限制滅活病毒鼻腔免疫效率的兩個主要瓶頸。樹突狀細胞伸出突起通過上皮細胞連線攝取抗原呈遞給淋巴細胞是啟動黏膜免疫的關鍵。因此，本項目主要從促進滅活病毒的吸收和誘導鼻腔黏膜樹突狀細胞的分化和成熟提高鼻腔局部和全身免疫反應的效率。首先進行樹突狀細胞的分離和培養，然後建立呼吸道上皮細胞模型calu-3細胞與樹突狀細胞共同培養體系，研究影響共同培養模型中樹突狀細胞攝取滅活禽流感病毒的因素，最後套用體外試驗（細胞共培養模型）和體內（小鼠和雛雞）試驗一起證明CpG、膽酸鈉配合滅活禽流感病毒對樹突狀細胞分化和成熟、鼻腔局部細胞因子、局部Toll樣受體以及局部和全身血清中特異性抗體的影響。本研究結果將為滅活病毒進行鼻腔免疫提供充分的理論依據和預防禽流感發生提供一條簡便的免疫途徑
