畢昌昊，博士，中國科學院天津工業生物技術研究所，研究員。
基本介紹
- 中文名：畢昌昊
- 國籍：中國
- 畢業院校：南開大學
- 職業：科研工作者
人物簡介,研究方向,主要成就,代表論著,科研項目,
人物簡介
教育經歷：
2009 佛羅里達大學, Gainesville, FL，美國，理學博士，微生物與細胞生物學
2004 南開大學, 天津，科學碩士，生物化學與分子生物學
2001 南開大學, 天津，科學學士,生物化學
工作經歷：
2011至2013，The Joint BioEnergy Institute (JBEI), 美國勞倫斯伯克利國家實驗室, 研究生物學家
2009至2011，德拉瓦大學, 化工系,博士後研究員
研究方向
合成生物技術小組的目標是通過開發使能技術來推動合成生物研究和套用的極限。我們專注於DNA和基因組操作技術，以更快、更深、更大的方式控制DNA。主要研究領域是合成生物學技術，重點發展和套用新的方法和技術，如基因組和鹼基編輯技術、體內DNA進化和突變技術以及人工微生物細胞器和人工生命形式的構建。
主要成就
2024年1月，《自然-生物技術》刊登了新一代鹼基編輯器相關成果，文章表明，中國科學院天津工業生物技術研究所畢昌昊研究員帶領的合成生物技術研究團隊和張學禮研究員帶領的微生物代謝工程研究團隊開發了不依賴脫氨酶（DAF）的鹼基編輯器DAF-CBE和DAF-TBE，在大腸桿菌和哺乳動物細胞中實現了高效的鹼基顛換編輯。
代表論著
1.Wang, W., He, P., Zhao, D., Ye, L., Dai, L., Zhang, X*., Bi, C*. (2019). Construction of Escherichia coli cell factories for crocin biosynthesis. Microbial cell factories, 18(1), 120. 二區
2. Wu, T., Li, S,Zhang, X*,Bi, C*. (2019). Engineering an Artificial Membrane Vesicle Trafficking System (AMVTS) for the Excretion of β-Carotene in Escherichia coli. ACS Synth. Biol 二區
3.Wu, Z., Wang, J., Zhang,Zhang, X*,Bi, C*. (2019). Engineering an electroactive Escherichia coli for the microbial electrosynthesis of succinate by increasing the intracellular FAD pool. Biochemical Engineering Journal. 146(132-142) 二區
4.Wu, Z., Wang, J., Liu, J., Wang, Y., Bi, C*., Zhang, X*. (2019). Engineering an electroactive Escherichia coli for the microbial electrosynthesis of succinate from glucose and CO 2.Microbial cell factories,18(1), 15. 二區
5. Li, Z., Xiong, B., Liu, L., Li, S., Xin, X., Li, Z., Zhang, X*, Bi, C*. (2019). Development of an autotrophic fermentation technique for the production of fatty acids using an engineered Ralstonia eutropha cell factory.Journal of industrial microbiology & biotechnology, 1-8. 二區
6. Ye, L., Zhu, X., Wu, T., Wang, W., Zhao, D.,Bi, C. *., Zhang, X*. (2018). Optimizing the localization of astaxanthin enzymes for improved productivity. Biotechnology for biofuels, 11(1), 278. 一區
7. Xiong, B., Li, Z., Liu, L., Zhao, D., Zhang, X*., Bi, C*. (2018). Genome editing of Ralstonia eutropha using an electroporation-based CRISPR-Cas9 technique.Biotechnology for Biofuels,11(1), 172. 一區
8. Wu, T., Li, S., Zhang, B., Bi C*, Zhang X*. (2018). Engineering Saccharomyces cerevisiae for the production of the valuable monoterpene ester geranyl acetate.Microbial cell factories,17(1), 85. 二區
9. Wu, T., Ye, L., Zhao, D., Li, S., Li, Q., Zhang, B*., Bi, C*. (2018). Engineering membrane morphology and manipulating synthesis for increased lycopene accumulation in Escherichia coli cell factories.3 Biotech,8(6), 269. 三區
10. Feng, X., Zhao, D., Zhang, X., Ding, X*., & Bi, C*. (2018). CRISPR/Cas9 assisted Multiplex Genome Editing Technique in Escherichia coli.Biotechnology journal, 二區
11. Zhao, D., Feng, X., Zhu, X., Wu, T., Zhang, X*., andBi, C*. (2017) CRISPR/Cas9-assisted gRNA-free one-step genome editing with no sequence limitations and improved targeting efficiency, Scientific Reports. 2017. 16624. 三區
12. Zhu X, Zhao D, Qiu H, Fan F, Man S,Bi C*, Zhang X*(2017). The CRISPR/Cas9-facilitated multiplex pathway optimization (CFPO) technique and its application to improve the Escherichia coli xylose utilization pathway.Metab Eng. 43:37-45. 一區
13. Wu T, Ye L, Zhao D, Li S, Li Q,Bi C*, Zhang X*. (2017) Membrane engineering - A novel strategy to enhance the production and accumulation of β-carotene in Escherichia coli.Metab Eng., 43: 85-91. 一區
14. Li Q, Fan F, Gao X, Yang C, Bi C, Tang J, Liu T, Zhang X*. (2017) Balanced activation of IspG and IspH to eliminate MEP intermediate accumulation and improve isoprenoids production in Escherichia coli. Metab Eng.08.005 一區
15. Ye L, He P, Li Q, Zhang X*, Bi C*. (2017) Type IIs restriction based combinatory modulation technique for metabolic pathway optimization. Microb Cell Fact. 16:47. 二區
16. Xiao M, Zhu X, Xu H, Tang J, Liu R, Bi C, Fan F*, Zhang X*. (2017) A novel point mutation in RpoB improves osmotolerance and succinic acid production in Escherichia coli. BMC Biotechnology. 17:10 二區
17. Li J, Zhu, X Chen, J Zhao, D., Zhang X*, Bi, C*. (2017) Construction of a novel anaerobic pathway in Escherichia coli for propionate production. BMC biotechnology17(1), 38. 二區
18. Zhao D, Yuan S, Xiong B, Sun H, Ye L, Li J, Zhang X*, Bi C*. (2016) Development of a fast and easy method for Escherichia coli genome editing with CRISPR/Cas9Microb Cell Fact. 15:205. 二區
19. Ye L, Zhang C, Bi C, Li Q*, Zhang X*. (2016) Combinatory Optimization of Chromosomal Integrated Mevalonate Pathway for β-carotene Production in Escherichia coli. Microb Cell Fact. 2016, 15:202.6 二區
20. Li S, Ding W,Zhang X, Jiang H*, Bi C*. (2016) Development of a modularized two-step (M2S) chromosome integration technique for integration of multiple transcription units in Saccharomyces cerevisiae. Biotechnol Biofuels. 2016, 9:232 一區
21. Sun H, Zhao D, Xiong B, Zhang C*, Bi C*. (2016). Engineering Corynebacterium glutamicum for violacein hyper production. Microb Cell Fact.15(1), 148. 二區
科研項目
主持（首席科學家）863計畫項目“生物質的快速氣化與生物轉化關鍵技術”。
