基本介紹
- 中文名：王盪
- 學位/學歷：博士
- 職業：教師
- 專業方向：動物病毒分子生物學
- 任職院校：華中農業大學動物科學技術學院動物醫學院
個人經歷,研究方向,學術成果,
個人經歷
教育經歷
2002.09 - 2006.06， 華中農業大學，生物科學（國家生物學基地班），理學學士；
2006.09 - 2011.06， 華中農業大學，預防獸醫學，農學博士。
工作經歷
2011.07-2013.06，華中農業大學生物學博士後流動站，博士後；
2013.07-2015.12，華中農業大學動物醫學院預防獸醫學系，講師；
2016.01-至今，華中農業大學動物醫學院預防獸醫學系，副教授，碩士研究生導師。
研究方向
動物病毒分子生物學
學術成果
1.Wang D, Fang L, Shi Y, Zhang H, Gao L, Peng G, et al. Porcine Epidemic Diarrhea Virus 3C-Like Protease Regulates Its Interferon Antagonism by Cleaving NEMO. Journal of Virology. 2016; 90(4):2090-101.
2.Wang D, Fang L, Wei D, Zhang H, Luo R, Chen H, et al. Hepatitis A virus 3C protease cleaves NEMO to impair induction of beta interferon. Journal of Virology. 2014; 88(17):10252-8.
3.Wang D, Fang L, Li K, Zhong H, Fan J, Ouyang C, et al. Foot-and-mouth disease virus 3C protease cleaves NEMO to impair innate immune signaling. Journal of Virology. 2012; 86(17):9311-22.
4.Wang D, Fang L, Li P, Sun L, Fan J, Zhang Q, et al. The leader proteinase of foot-and-mouth disease virus negatively regulates the type I interferon pathway by acting as a viral deubiquitinase. Journal of Virology. 2011; 85(8):3758-66.
5.Zhou J, Wang D*, Xi Y, Zhu X, Yang Y, Lv M, Luo C, Chen J, Ye X, Fang L, Xiao S. Assessing activity of Hepatitis A virus 3C protease using a cyclized luciferase-based biosensor. Biochemical and Biophysical Research Communications. 2017; 488(4):621-627.
6.Zhu X, Fang L, Wang D*, Yang Y, Chen J, Ye X, Foda MF, Xiao S*. Porcine deltacoronavirus nsp5 inhibits interferon-beta production through the cleavage of NEMO. Virology. 2017; 502:33-8.
7.Wang D, Fang L, Xiao S Porcine epidemic diarrhea in China. Virus Research. 2016; 226:7-13.
8.Shi Y, Wang D*, Zhu X, Wu Q, Chen H, Xiao S, Fang L*. Molecular cloning and functional characterization of porcine E74-like factor 4 (ELF4). Developmental and Comparative Immunology. 2016; 65:149-58.
9.Zhang H, Ouyang H, Wang D*, Shi J, Ouyang C, Chen H, Fang L*, et al. Mycobacterium tuberculosis Rv2185c contributes to nuclear factor-kappaB activation. Molecular Immunology. 2015; 66(2):147-53.
10.Wang D#, Fan J#, Fang L, Luo R, Ouyang H, Ouyang C, et al. The nonstructural protein 11 of porcine reproductive and respiratory syndrome virus inhibits NF-kappaB signaling by means of its deubiquitinating activity. Molecular Immunology. 2015; 68(2PtA):357-66.
11.Duan E#, Wang D#, Fang L, Ma J, Luo J, Chen H, et al. Suppression of porcine reproductive and respiratory syndrome virus proliferation by glycyrrhizin. Antiviral Research. 2015; 120:122-5.
12.Shi J, Zhang H, Fang L, Xi Y, Zhou Y, Luo R, et al Wang D*. A novel firefly luciferase biosensor enhances the detection of apoptosis induced by ESAT-6 family proteins of Mycobacterium tuberculosis. Biochemical and Biophysical Research Communications. 2014; 452(4):1046-53.
13.Zhong H#, Wang D#, Fang L, Zhang H, Luo R, Shang M, et al. Ubiquitin-specific proteases 25 negatively regulates virus-induced type I interferon signaling. PloS One. 2013; 8(11):e80976.
14.Wang D#, Cao L#, Xu Z#, Fang L, Zhong Y, Chen Q, et al. MiR-125b reduces porcine reproductive and respiratory syndrome virus replication by negatively regulating the NF-kappaB pathway. PloS One. 2013; 8(2):e55838.
15.Zeng S#, Wang D#, Fang L, Ma J, Song T, Zhang R, et al. Complete coding sequences and phylogenetic analysis of porcine bocavirus. Journal of General Virology. 2011; 92(Pt 4):784-8
16.Wang D, Fang L, Zhao F, Luo R, Chen H, Xiao S. Molecular cloning, expression and antiviral activity of porcine interleukin-29 (poIL-29) . Developmental and Comparative Immunology. 2011; 35(3):378-84.
17.Wang D, Fang L, Liu L, Zhong H, Chen Q, Luo R, et al. Foot-and-mouth disease virus (FMDV) leader proteinase negatively regulates the porcine interferon-lambda1 pathway. Molecular Immunology. 2011; 49(1-2):407-12.
18.Wang D, Fang L, Li P, Chen Q, Luo R, Chen H, et al. Molecular cloning of the porcine RANTES promoter: functional characterization of dsDNA/dsRNA response elements in PK-15 cells. Developmental and Comparative Immunology. 2011; 35(3):345-51.
19.Wang D, Fang L, Bi J, Chen Q, Cao L, Luo R, et al. Foot-and-mouth disease virus leader proteinase inhibits dsRNA-induced RANTES transcription in PK-15 cells. Virus Genes. 2011; 42(3):388-93.
20.Wang D, Fang L, Luo R, Ye R, Fang Y, Xie L, et al. Foot-and-mouth disease virus leader proteinase inhibits dsRNA-induced type I interferon transcription by decreasing interferon regulatory factor 3/7 in protein levels. Biochemical and Biophysical Research Communications. 2010; 399(1):72-8.
21.Wang D, Fang L, Li T, Luo R, Xie L, Jiang Y, Chen H, Xiao S. Molecular cloning and functional characterization of porcine IFN-beta promoter stimulator 1 (IPS-1) . Vet Immunol Immunopathol. 2008;125(3-4):344-53.
22.王 盪, 方六榮, 羅 銳, 謝立蘭, 江雲波, 陳煥春, 肖少波. 豬IFN-β啟動子及其NF-κB結合位點螢光素酶報告載體的構建與活性檢測. 中國獸醫學報. 2010, 30 (1):107-109.
23.王 盪, 方六榮, 梅小偉, 謝立蘭, 陳煥春, 肖少波. 豬維甲酸誘導基因I 的克隆及其在誘導I 型干擾素中的作用. 畜牧獸醫學報. 2010, 41 (2):135-140.
科研項目
1. 豬流行性腹瀉病毒3C樣蛋白酶切割干擾素信號通路關鍵蛋白的分子機制 2017.09
2. 抗PRRS病毒廣譜中和抗體的篩選及其生物學特性研究 2017.07
3. 動脈炎病毒PRRSV和EAV編碼的3C樣蛋白酶切割NEMO的分子機制 2017.01
4. 抗PRRSV增殖的宿主限制因子的篩選與鑑定 2016.01
