王戰輝，男，1979年6月出生，博士，中國農業大學動物醫學院研究員，博士生、碩士生導師，入選2012年教育部新世紀優秀人才支持計畫。
基本介紹
- 中文名：王戰輝
- 出生日期：1979年6月
- 學位/學歷：博士
- 任職院校：中國農業大學
研究方向,學術成果,科研項目,發表論文,獲獎記錄,
研究方向
主要從事小分子有害化合物（獸藥、黴菌毒素、非法添加劑）的抗體製備、快速檢測技術及分子識別機制研究。
學術成果
科研項目
近五年主持參加的科研項目30餘項，申請國家發明專利30餘項，參與制定國家標準10餘項。
發表論文
國內外學術刊物發表研究論文100餘篇，SCI收錄80餘篇。其代表論文有：
1. Zhanhui Wang, Tiejun Mi, Huiyan Zhang, Yajie Sheng, Weimin Shi, Suxia Zhang, Jianzhong Shen. Hapten synthesis, monoclonal antibody production, and development of a competitive indirect enzyme-linked immunosorbnent assay for erythromycin in milk. Food Chemistry, 2015, 171, 98-107.
2. Xiao Liang, Hengjia Ni, Ross C. Beier, Yanni Dong, Jingya Li, Xiangshu Luo, Suxia Zhang, Jianzhong Shen, Zhanhui Wang*. Highly broad-specific and sensitive enzyme-linked immunosorbent assay for screening of the sulfonamides: Assay optimization and application to milk samples. Food Analytical Methods, 2014, 7, 1992-2002.
3. Zhanhui Wang, Qi Liang, Jianzhong Shen*. A Novel Ligand for the Affinity-Chromatographic Purification of Monoclonal Antibody to Ractopamine. Journal of Chmatography B, 2014, 971, 10-13.
4. Zhanhui Wang, Heng Li, Chenglong Li, Qing Yu, Jianzhong Shen*, Sarah De Saeger. Development and application of a quantitative fluorescence-based immunochromatographic assay for fumonisin B1 in maize. Journal of Agricultural Food and Chemistry, 2014, 62(27): 6294–6298.
5. Beloglazova N.V., Speranskaya E.S., Wu A, Zhanhui Wang, Sanders M, Goftman V., Zhang D. Goryacheva I.Yu., De Saeger S. Novel multiplex fluorescent immunoassays based on quantum dot nanolabels for mycotoxins determination . Analytical Chemistry, 2014, 62, 59-65.
6. Tiejun Mi, Suxia Zhang a, Jianzhong Shen, Sergei A. Eremin, Zhanhui Wang*. Development and Optimization of Fluorescence Polarization Immunoassay for Orbifloxacin in Milk. Analytical methods, 2014, 6 (11), 3849 - 3857.
7. Yajie Sheng, Sergei A Eremin,Tiejun Mi, Suxia Zhang, Jianzhong Shen, Zhanhui Wang*. The development of a fluorescence polarization immunoassay for aflatoxin detection. Biomedical and Environmental Sciences, 2014, 27(2): 126-129.
8. Zhanhui Wang, Huiyan Zhang, Hengjia Ni, Suxia Zhang, Jianzhong Shen. Development of a highly sensitive and specific immunoassay for enrofloxacin based on heterologous coating haptens. Analytica Chimica Acta. 2014, 820(11): 152–158.
9. Elena S Speranskaya, Natalia V Beloglazova, Pieterjan Lenain, Sarah De Saeger, Zhanhui Wang, Suxia Zhang, Zeger Hens, Dietmar Knopp, Reinhard Niessner, Dmitry V Potapkin, Irina Yu Goryacheva. Polymer-coated fluorescent CdSe-based quantum dots for application in immunoassay. Biosensors & bioelectronics. 2014, 53(15): 225-231.
10. Wenxiao Jiang, Huiyan Zhang, Xiangmei Li, Xinxin Zhang, Suxia Zhang, Jianzhong, Shen, Zhanhui Wang*. Monoclonal antibody production and the development of an indirect competitive enzyme-linked immunosorbent assay for screening spiramycin in milk. Journal of Agricultural and Food Chemistry, 2013, 61 (46), 10925–10931
11. Rui Chen, Heng Li, Han Zhang, Suxia Zhang,Weimin Shi, Jianzhong Shen, Zhanhui Wang* Development of a lateral flow fluorescent microsphere immunoassay for the determination of sulfamethazine in milk. Analytical Bianalytical Chemistry, 2013, 405(21): 6783-6789.
12. Zhanhui Wang, Yonghan Li, Xiao Liang, Suxia Zhang, Weimin Shi, Jianzhong Shen. Forcing immunoassay for sulfonamides to higher sensitivity and broader detection spectrum by site heterologous hapten inducing affinity improvement. Analytical Methods, 2013, 5, 6990-7000.
13. Xiao Liang, Zhanhui Wang, Chunmei Wang, Kai Wen, Tiejun Mi, Jing Zhang, Suxia Zhan. A proof-of-concept receptor-based assay for sulfonamides. Analytical Biochemistry, 2013,438, 110-116. Co-first author,
14. Zhanhui Wang, Ross C. Beier, Yajie Sheng, Suxia Zhang, Wenxiao Jiang, Zhaopeng Wang, Jin Wang, Jianzhong Shen. Monoclonal antibodies with group-specificity toward sulfonamides: selection of hapten and antibody selectivity. Analytical Biochemical Chemistry, 2013, 405(12): 4027-4037.
15. Zhanhui Wang, Na Li, Suxia Zhang, Shuangyang Ding, Jianzhong Shen.Production of antibodies and development of enzyme-linked immunosorbent assay for valnemulin in porcine liver. Food Additives and Contaminants. 2013, 30(2):244-252.
16. Jing Zhang, Zhanhui Wang, Kai Wen, Xiao Liang, Jianzhong Shen. Penicillin-binding protein 3 of Streptococcus pneumonia and its application in screening of β-lactams in milk, Analytical Biochemistry, 2013, 442(2), 158-165.
17. Tiejun Mi, Zhanhui Wang, Sergei A. Eremin, Jianzhong Shen, Suxia Zhang*. Simultaneous Determination of Multiple (Fluoro) quinolone Antibiotics in Food Samples by A One-Step Fluorescence Polarization Immunoassay. Journal of Agricultural and Food Chemistry, 2013, 61 (39), 9347–9355.
18. Wenxiao Jiang, Zhanhui Wang; Beier, R. C.; Jiang, H.; Wu, Y.; Shen, J., Simultaneous Determination of 13 Fluoroquinolone and 22 Sulfonamide Residues in Milk by a Dual-Colorimetric Enzyme-Linked Immunosorbent Assay. Anal Chem 2013, 85 (4), 1995-1999.
19. Jia Wang, Zhanhui Wang, Jing Liu, Hao Li, Qing X. Li, Ji Li, Ting Xu. Nanocolloidal gold-based immuno-dip strip assay for rapid detection of Sudan red I in food samples. Food Chemistry, 2013,136: 1478–1483
20. 王戰輝,米鐵軍,沈建忠. 螢光偏振免疫分析檢測糧食及其製品中的真菌毒素研究進展. 中國農業科學. 2012, 45(23):4862-4872.
21. Yajie Sheng, Wenxiao Jiang, Sarah De Saeger, Jianzhong Shen, Suxia Zhang, Zhanhui Wang*. Development of a sensitive enzyme-linked immunosorbent assay for the detection of fumonisin B1 in maize. Toxicon. 2012, 60(7):1245–1250
22. Yanshen Li, Weimin Shi, Jianzhong Shen, Suxia Zhang, Linli Cheng and Zhanhui Wang*. Development of a rapid competitive indirect ELISA procedure for the determination of deoxynivalenol in cereals. Food and Agricultural Immunology. 2012, 23(1): 41-49
23. Zhanhui Wang, Zhenpeng Kai, Ross C. Beier, Jianzhong Shen, Xinling Yang . Investigation of Antigen-Antibody Interactions of Sulfonamides with a Monoclonal Antibody in a Fluorescence Polarization Immunoassay Using 3D-QSAR Models. International Journal of Molecular Sciences. International Journal of Molecular Sciences, 2012, 13, 6334-6351.
24. Yanshen Li, Zhanhui Wang, Sarah De Saeger, Weimin Shi, Cun Li, Suxia Zhang, Xingyuan Cao, Jianzhong Shen. Determination of deoxynivalenol in cereals by immunoaffinity clean-up and ultra-high performance liquid chromatography tandem mass spectrometry. Method. 2012, 56(2): 192-197 Co-author
25. Zhanhui Wang, Pengjie Luo, Linli Cheng, Suxia Zhang, Jianzhong Shen. Hapten–antibody recognition studies in competitive immunoassay of a-zearalanol analogs by computational chemistry and Pearson Correlation analysis 2011, Journal of Molecular Recognition. 24(5):815-823.
26. Zhanhui Wang, Jing Zhang, Suxia Zhang, Jianzhong Shen. Heterologous structure of coating antigen on sensitivity of ELISA for sulfamethazine: evidence from molecular similarity analysis. 2011, Food and Agricultural Immunology. 22(2):115-124
27. Zhanhui Wang, Linli Cheng, Weimin Shi, Suxia Zhang, Jianzhong Shen. Fluorescence Polarization Immunoassay for Salinomycin Based on Monoclonal Antibody. 2010. Science in China Series B-Chemistry.53(3):553-555
28. Zhanhui Wang, Xingyuan Cao, Zhenpeng Kai, Ross C. Beier, Congming Wu, Haiyang Jiang, Linli Cheng, Jianzhong Shen*, Xinling Yang. Three Dimensional Quantitative Structure-Activity Relationships of Sulfonamides Binding Monoclonal Antibody by Comparative Molecular Field Analysis. 2008, Nature Precedings (2008)
29. Cun Li*, Zhanhui Wang*, Xingyuan Cao, Ross C. Beier, Suxia Zhang, Shuangyang Ding, Xiaowei Li, and Jianzhong Shen, Development of an Immunoaffinity Column Method Using Broad-Specificity Monoclonal Antibodies for Simultaneous Extraction and Cleanup of Fluoroquinolone and Sulfonamide Antibiotics in Animal Muscle Tissues. 2008, Journal of Chromatography A, 1209(1-2), 1-9.（*co-first author）
30. Zhanhui Wang, Suxia Zhang, Nailya R. Murtazina, Sergei A. Eremin, Jianzhong Shen, Determination of the veterinary drug maduramicin in food by Fluorescence Polarization Immunoassay. 2008, International Journal of Food Science and Technology, 43, 114-122.
31. Zhanhui Wang, Suxia. Zhang, Shuangyang Ding, Seiger. A. Eremin, Jianzhong Shen. Simultaneous determination of sulphamerazine, sulphamethazine and sulphadiazine in honey and chicken muscle by fluorescence polarisation immunoassay based on monoclonal antibody. Food Additives and Contaminants, 2008, 25 (5): 574-582.
32. 王戰輝，丁雙陽，張素霞, 沈建忠. 分子模擬技術研究17種磺胺類藥物與抗體親和力構效關係. 2008. 化學學報. 66 (23): 2613-2619.
33. 王戰輝，張素霞, 沈建忠. 螢光偏振免疫分析檢測磺胺甲基嘧啶研究. 2008, 光譜學與光譜分析. 28(7):1621-1625.
34. 王戰輝，張素霞，丁雙陽，沈建忠. 螢光偏振免疫分析法研究17種磺胺類藥物與抗體親和力的構效關係. 2008, 高等學校化學學報. 29(6): 1107-1111.
35. Zhanhui Wang, Yan Zhu, Shuangyang Ding, Fangyang He, Ross C.Beier, Jiancheng Li, Haiyang Jiang, Caiwei Feng, Yuping Wan, Suxia Zhang, Zhenpeng Kai, Xinling Yang, Jianzhong Shen. Development of a Monoclonal Antibody-Based Broad-Specificity ELISA for Fluoroquinolone Antibiotics in Foods and Molecular Modeling Studies of Cross-Reactive Compounds. 2007, Analytical Chemistry, 79 (12): 4471-4478.
36. Zhanhui Wang, Suxia Zhang, Irina S. Nesterenkob, Sergei A. Ereminb, Jianzhong Shena,Monoclonal Antibody-Based Fluorescence Polarization Immunoassay for Sulfamethoxypyridazine and Sulfachloropyridazine. 2007, Journal of Agriculture and Food Chemistry, 55(17): 6871- 6878.
獲獎記錄
2008年獲第一屆“北京市優秀博士論文”，2009年獲全國百篇優秀博士論文提名獎。
