《海洋生物技術原理和套用》是1997年海洋出版社出版的圖書，作者是張士璀、馬軍英、范曉，。
基本介紹
- 書名：海洋生物技術原理和套用
- 作者：張士璀、馬軍英、范曉
- ISBN：7-5027-4225-5
- 頁數：294
- 出版社：海洋出版社
- 出版時間：1997-01-01
- 裝幀：平裝
圖書目錄
1 緒論...... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ( 1)
1.1 何謂海洋生物技術........................... ................................................... ( 1)
1.2 海洋生物技術研究現狀........................................................................ ( 2)
2 海洋微生物的篩選和培養........................................................................... ( 6)
2.1 概論... ... ... ...... ............... ... ... ......... ... ... ............ ... ... ... ............ ... ... ...... ( 6)
2.2 海洋微生物的採樣、分離和培養技術...................................................... ( 7)
2.3 細菌的分類、鑑定與保存................................................................ . .(13)
2.4 產生EPA的海洋細菌的篩選............................................................... (19)
2.5 油細菌的篩選.................................................................................... (20)
2.6 海洋藍藻的採集、分離和培養............................................................... (22)
3 海洋動物細胞的培養.................. ......... ... ... ... ...... ...... ... ... ... ... ...... ...... ... ... ... (28)
3.1 概論.................................................................. ......................... . .(28 )
3.2 鰻鱺、鯉魚肝細胞的培養................................................................ . .(28)
3.3 鰻鱺肝細胞灌流法的操作................................................................ . .(29)
3.4 分離鰻鱺肝細胞的原代單層培養法......................................................... (34)
3.5 振盪法分離鯉魚肝細胞................................................................... . .(35)
3.6 分離鯉魚肝細胞的原代單層培養............................................................ (35)
3.7 培養肝細胞的實驗........................ .................. ............................... . .(3 6)
3.8 草魚腎組織細胞的原代培養和傳代培養實驗............................................. (39)
4 大型海藻的組織培養............................................................................ . .(4 1)
4 .1 概論. . ... ......... ... ... ... ... ......... ... ... ... ... ... ... ... ... ... .. ... ... ... ... ... ... ... . . .(41 )
4.2 組織培養的設備和基本技術............................................................. . .(43)
4.3 外植體的無菌化技術...................................................................... . .(48)
4.4 海藻組織塊愈傷組織的誘導............................................. .................... (49)
4.5 無菌培養系的愈傷組織誘導.................................................................. (50)
4.6 細胞培養.......................................................................................... (51)
5 海洋動物的細胞操作........................ ......................................................... (56)
5.1 概論... ................................................... .......................................... (56)
5.2 性決定(性別控制)..................... ......................................................... (59)
5.3 嵌合體動物的培育.............................. ................................................ (62)
6 大型海藻的細胞操作............ ... ... ... ......... ... ... ............................ ... ......... ... ... (65)
6.1 概論................................................................................................ (65)
6.2 大型海藻的培養................................................................................. (68)
6.3 原生質體分離技術......................................................................... . .(70)
6.4 原生質體培養技術......................................................................... . .(74)
6.5 細胞融合技術............................................................................... . .(77)
6.6 異種融合細胞的篩選及個體再生............................................................ (82)
7 海洋微藻的細胞操作... ... ............... ... ... ......... ... ... ............ ... ... ............ ......... (85)
7.1 概論............................................. ........................... ... ..................... (85)
7.2 海洋微藻的分離和純化技術.................................................................. (88)
7.3 培養基.................................... ......................................................... (91)
7.4 海洋微藻的生長測量和培養技術............................................................ (96)
7.5 原生質體分離技術........................................................................... (100)
7.6 原生質體培養技術.................. ......................................................... (103)
7.7 細胞融合技術................................................................................. (104)
7.8 細胞學技術................................................................................ .(105)
8 海產魚類的染色體操作........................................................................... (109)
8.1 概論... ................................................... ....................................... (109)
8.2 天然多倍體......... ...... ............ ... ......... ... ... ... ... ...... ... ... ... ............ ...... (109)
8.3 人工誘導的多倍體...... ..................................................................... (111)
8.4 單性發育................................................................................... .(118)
8.5 染色體片斷移植.............................................................................. (123)
8.6 染色體操體技術的套用及其發展趨勢......................................... . . . .(123)
9 海產無脊椎動物的染色體操作......... ......................................................... (129)
9.1 概論... ............ ... ... ............ ...... ...... ... ... ... ......... ... ... ............ ... ......... (129)
9.2 細胞核及染色體的觀察方法............................................................... (132)
9.3 人工多倍體誘導法........................................................................... (135)
9.4 多倍體的鑑定................................................................................. (137)
10 海洋動物的基因克隆.............................................................................. (141)
10.1 概論... ... ... ... ... ... ... ... ...... ... ... ... ......... ... ... ... ... ... ... ......... ... ... ...... ...... (141)
10.2 海洋動物的cDNA 克隆.................................................................. (143)
10.3 目的cDNA克隆的鑑定.................................................................. (166)
10.4 DNA 序列測定.............................................................................. (181)
11 轉基因海洋動物........................ ............................................................ (190)
11.1 概論..................................................................... ........................ (190 )
11.2 基因導入方法................................................................................. (195)
11.3 轉基因動物的鑑別...................................................................... . . (198)
12 海洋微藻的基因工程...... ........................................................................ (200)
12.1 概論......... ... ... ... ...... ...... ... ... ............ ... ... ............ ... ... ... ............... ... (200)
12.2 載體的開發............................................................... ..................... (201)
12.3 受體系統.......................................................... ....................... . . (202)
12.4 外源基因轉移方法........................................................................... (203)
13 大型海藻的基因工程................................. ......... . . ............................. (208)
13.1 概論............................................................................................. (208)
13.2 材料的選擇.................................................................................... (210)
13.3 大型海藻的核酸製備............................................. ........................... (210)
13.4 大型海藻的目的基因................................................................... . . (211)
14 純種保存... ... ... ... ... ... ... ... ... ... ... ...... ... ......... ... ... ... ... ... ... ... ... ... ... ... ... ... ... (213)
14.1 概論............................................................................................. (213)
14.2 大型藻類的純種保存........................................................................ (215)
14.3 海洋微型生物的冷凍保存.................................................................. (217)
14.4 海洋植物的冷凍保存........................................................................ (220)
14.5 海洋動物生殖細胞的冷凍保存............................................................ (222)
15 生物反應器.......................................................................................... (225)
15.1 概論............................................................................................. (225)
15.2 微生物固定化......... ............................................................ ............ (226)
15.3 利用生物反應器生產有用物質............................................................ (228)
15.4 海洋藍藻生物反應器........................................................................ (231)
16 生物感測器... ... ......... ... ... ... ... ......... ... ... .................. ............ ... ... ... ......... (235)
16.1 概論............................................................................................. (235)
16.2 接受器的固定化.............................................................................. (237)
16.3 換能器.......................................................................................... (239)
16 .4 套用... . .. . .. .. . . . . . . . .. . .. . . . .. . .. . . .. . .. .. . . .. . .. . . . . . . .. . .. . . .. . . . .. . . .. . . . .. . . .. . . . (24 1)
16.5 結語.............................................................................. ............... (243)
17 海洋環境保護............................................................... ........................ (245)
17.1 概論............................................................................................. (245)
17.2 石油的生物降解.............................................................................. (247)
17.3 鹵代烴的生物降解........................................................................... (248)
17.4 酶工程方法消除工業廢水中的酚與芳香胺............................................. (250)
17.5 藻類對重金屬離子的吸附.................................................................. (253)
17.6 可降解塑膠................................................................................ .(255)
17.7 生物技術在解決生物耗氧化合物對環境污染方面的套用........................... (256)
18 疾病的診斷與防治................................................................................. (257)
18.1 概論............................................................................................. (257)
18.2 診斷........................... .................................................................. (258)
18.3 防治............................................................................................. (260)
19 海洋生物活性物質... ... ... ... ... ... ... ... ... ...... ... ...... ... ...... ... ... ... ... ... ... ...... ... ... (261)
19. 1 概論... ... ... ... ... ... ... ... ... ... .... . ....... ... ... ... ... ...... ... ... ... ... ... ... ...... .. .(261)
19.2 海洋生物活性物質的研究方法........................................................ .(267)
20 海洋新材料— —甲殼質....................................................................... .(271)
20.1 概論... ... ...... ... ... ... ... ... ... ... ... ... ... ... ... ... ... ...... ... ... ... ... ... ... ... ... ... ... ... (271)
20.2 人工皮膚............ ........................................................................... (272)
