李朋富,男,任教專業為理學-生物科學類,南京大學生命科學院副教授。
基本介紹
- 中文名:李朋富
- 專業方向:藍藻多糖的生化特性
- 任職院校:南京大學
- 職稱:副教授
學術兼職,研究方向,獲獎情況,科研成果,論文,專利,
學術兼職
中國藻類學會理事,中國微生物學會會員。
研究方向
(1)藍藻多糖的生化特性、合成基因簇的鑑定、生理生態功能以及生物技術套用
藍藻(又叫藍細菌)產生大量的多糖,特別是胞外多糖,關於其生物學功能以及資源化利用的研究不多。相比於其它生物多糖,藍藻多糖含有的單糖種類更多,分子結構也更為複雜,通過分析其絮凝活性、分散活性、乳化活性、凝膠特性和粘度特性等,從而研究其在藍藻群體形成、底棲生長、浮游生長以及抵禦外界不良環境等過程中的作用機制。藍藻多糖分子結構複雜,因而可能會具有不同於其它生物多糖的物化特性和生物活性,從而可能會有新的潛在套用價值,如作為新的絮凝劑、乳化劑、分散劑和凝膠等在醫藥和食品等行業套用。對於藍藻生物合成基因的研究目前處於空白,通過基因敲除的方法鑑定藍藻多糖基因合成簇是研究多糖合成的遺傳機制的基礎。
(2)藍藻水華暴發機制以及控制技術原理
藍藻水華已經成為一個世界性的重大環境問題,已越來越嚴重地危害著飲用水供應、漁業生產和旅遊等。我們發現水華暴發過程中藍藻基因型組成不斷地連續演替,是太湖藍藻能適應環境條件不斷變化導致水華長時間暴發和維持的重要機制,細菌在藍藻水華暴發過程中也發揮了重要影響。水華藍藻的最主要種類是微囊藻,微囊藻能形成水華的關鍵是其在自然界主要以群體形態存在,從而在抵禦浮遊動物攝食以及紫外線損害等方面具有競爭優勢,而微囊藻形成群體的關鍵因素是膠鞘多糖的作用,我們試圖通過分析膠鞘多糖的生化特性、多糖產生的生理和分子調控機制,研究膠鞘多糖在微囊藻群體形成過程中的作用機制。我們的研究也發現,群體大小不同的微囊藻基因型和毒素組成有差異,較大群體的產毒基因型比例較高,同時,較大群體含有的毒性高的毒素比例更高,因為群體大小不同的微囊藻在水體中的空間分布有差異,因此在進行水質安全監測的過程中必須特別警覺較大群體的微囊藻。
(3)微藻生物技術
微藻能光合自養生長,並且生長周期短,螺旋藻、鹽藻和血球藻等微藻已經進行工業化生產,並在保健品、天然色素以及藥物開發等方面成功套用。通過分離篩選出優良藻種,研究其在生物能源和生物活性物質等方面的生物技術套用。
(4)水生微生物多樣性以及生物技術套用
分析水生微生物的遺傳和生化多樣性,研究其在淨化水體方面的套用。
獲獎情況
教學
獲南京大學獎教金二等獎一次(1998年)、三等獎兩次(1997, 1999年)和單項獎一次(1996年)。
科研
1、獲2002年度南京大學“金桐石化科教發展基金”獎研金二等獎;
2、“藻類、細菌、真菌及其有機質(腐殖質)成礦模擬試驗“獲國家教育部科技進步三等獎(1999年,排名第四)。
科研成果
論文
代表性論文:
Weiguo Zhang, Peiliang Zhang, Hao Sun, Maozhen Chen, Shan Lu, Pengfu Li. (2014) Effects of various organic carbon sources on the growth and biochemical composition of Chlorella pyrenoidosa. Bioresource Technology 173, 52–58 (Corresponding author).
Weiguo Zhang, Min Liu, Peiliang Zhang, Fugen Yu, Shan Lu, Pengfu Li, Junying Zhou. (2014) Effects of paraquat on photosynthetic pigments, antioxidant enzymes, and gene expression in Chlorella pyrenoidosa under mixotrophic compared with autotrophic conditions. Archives of Environmental Contamination and Toxicology 67, 593–600 (Corresponding author).
Xingyu Wang, Mengjia Sun, Meijuan Xie, Min Liu, Lan Luo, Pengfu Li, Fanxiang Kong. (2013) Differences in microcystin production and genotype composition among Microcystis colonies of different sizes in Lake Taihu. Water Research 47, 5659-5669 (Corresponding author).
Xingyu Wang, Meijuan Xie, Wei Wu, Limei Shi, Lan Luo, Pengfu Li. (2013) Differential sensitivity of colonial and unicellular Microcystis strains to an algicidal bacterium Pseudomonas aeruginosa. Journal of Plankton Research 35, 1172–1176 (Corresponding author).
Jinmei Wang, Min Liu, Huijie Xiao, Wei Wu, Meijuan Xie, Mengjia Sun, Chenglin Zhu, Pengfu Li. (2013) Bacterial community structure in cooling water and biofilm in an industrial recirculating cooling water system. Water Science and Technology 68, 940-947 (Corresponding author).
Xingyu Wang, Mengjia Sun, Jinmei Wang, Letian Yang, Lan Luo, Pengfu Li, Fanxiang Kong. (2012) Microcystis genotype succession and related environmental factors in Lake Taihu during cyanobacterial blooms. Microbial Ecology 64, 986–999 (Corresponding author).
Li Chen, Tianli Li, Liang Guan, Yawei Zhou, Pengfu Li. (2011) Flocculating activities of polysaccharides released from the marine mat-forming cyanobacteria Microcoleus and Lyngbya. Aquatic Biology 11, 243-248 (Corresponding author).
Hualin Yang, Yuanfeng Cai, Meng Xia, Xingyu Wang, Limei Shi, Pengfu Li, Fanxiang Kong. (2011) Role of cell hydrophobicity on colony formation in Microcystis (Cyanobacteria). International Review of Hydrobiology 96, 141-148 (Corresponding author).
Li Chen, Xiao Men, Meirong Ma, Pengfu Li, Qingcai Jiao, Shan Lu, Fanxiang Kong, Shengcai Wu. (2010) Polysaccharide release by Aphanothece halophytica inhibits cyanobacteria/clay flocculation. Journal of Phycology 46, 417-423 (Corresponding author).
Limei Shi, Yuanfeng Cai, Zhuting Chen, Yawei Zhou, Pengfu Li, Fanxiang Kong. (2010) Diversity and abundance of aerobic anoxygenic phototrophic bacteria in two cyanobacterial bloom-forming lakes in China. Annales de Limnologie-International Journal of Limnology 46, 233-239 (Corresponding author).
Limei Shi, Yuanfeng Cai, Xingyu Wang, Pengfu Li, Yang Yu, Fanxiang Kong. (2010) Community structure of bacteria associated with Microcystis colonies from cyanobacterial blooms. Journal of Freshwater Ecology 25, 193-203 (Corresponding author).
Limei Shi, Yuanfeng Cai, Hualin Yang, Peng Xing, Pengfu Li, Lingdong Kong, Fanxiang Kong. (2009) Phylogenetic diversity and specificity of bacteria associated with Microcystis aeruginosa and other cyanobacteria. Journal of Environmental Sciences-China 21, 1581-1590 (Corresponding author).
Li Chen, Pengfu Li, Zhili Liu, Qingcai Jiao. (2009) The released polysaccharide of the cyanobacterium Aphanothece halophytica inhibits flocculation of the alga with ferric chloride. Journal of Applied Phycology 21, 327-331 (Corresponding author).
Pengfu Li, Yuanfeng Cai, Limei Shi, Lanfang Geng, Peng Xing, Yang Yu, Fanxiang Kong, Yujiong Wang. (2009) Microbial degradation and preliminary chemical characterization of Microcystis exopolysaccharides from a cyanobacterial water bloom of Lake Taihu. International Review of Hydrobiology 94, 645-655.
Limei Shi, Yuanfeng Cai, Pengfu Li, Haiwang Yang, Zhili Liu, Lingdong Kong, Yang Yu, Fanxiang Kong. (2009) Molecular identification of the colony-associated cultivable bacteria of cyanobacterium Microcystis aeruginosa and their effects on algal growth. Journal of Freshwater Ecology, 24, 211-218 (Corresponding author).
Peng-Fu Li, Zhi-Yong Mao, Xiang-Jun Rao, Xiao-Mei Wang, Mao-Zhong Min, Li-Wen Qiu, Zhi-Li Liu (2004) Biosorption of uranium by lake-harvested biomass from a cyanobacterium bloom. Bioresource Technology 94, 193-195.
Pengfu Li, Zhili Liu, Ren Xu. (2001) Chemical characterisation of the released polysaccharide from the cyanobacterium Aphanothece halophytica GR02. Journal of Applied Phycology 13, 71–77.
Morris GA, Li P, Puaud M, Liu Z, Mitchell JR, Harding SE. (2001) Hydrodynamic characterisation of the exopolysaccharide from the halophilic cyanobacterium Aphanothece halophytica GR02: a comparison with xanthan. Carbohydrate Polymer 44, 261–268.
Pengfu Li, Stephen E Harding, Zhili Liu. (2001) Cyanobacterial exopolysaccharides: their nature and potential biotechnological applications. Biotechnology and Genetic Engineering Reviews 18, 375–404.
專利
李朋富,陳麗,馬美榮,孟飛飛,劉志禮,焦慶才,“一種微生物多糖分散劑及其製備方法和套用”,2010年11月10日(授權),專利號:ZL 2008 1 0124020.4。
劉志禮,李朋富,許浩,陳麗,“集胞藻胞外多糖的製備及其作為增稠劑和乳化劑的套用”,2009年3月18日(授權),專利號:ZL 2006 1 0098098.4。