Primate Pluripotent Stem Cells and Mechanisms Controlling Self-Renewal
Pluripotent stem cells have important applications in regenerative medicine due to giving rise to all types of tissue cells in the body. The premise of stem cells applied in the regenerative medicine is to establish chimera competent and germline competent stem cells. Based on the establishment of primate naive-like stem cells with chimera-competency, our objectives are to investigate the mechanisms controlling naive state of stem cells and use the naive stem cells to regenerate important functional tissues and organsin vivoandin vitro.
Developing 3D defined and scalable protocols to culture primate stem cells
One obstacle hindering clinical applications of stem cells is lack of a protocol for stably and large-scaly expanding stem cells. Our laboratory tries to develop some novel defined conditions for stem cell culture and differentiation to reproductively produce sufficient number of functional cells for replacement therapy.
Stem cell therapy and corresponding molecular mechanisms of therapy
Evaluation of safety and function of stem cellsin vivoafter transplanted into non-human primate is one key for translating basic research into clinical therapy. As representative of the nervous system and heart system, we try to establish corresponding non-human primate models of neural diseases and myocardial infarction and use these animal models to evaluate long-term fate and function of stem cellsin vivo.
Lifeng Xiang, Yu Yin, Yun Zheng, Yanping Ma, Yonggang Li, Zhigang Zhao, Junqiang Guo, Zongyong Ai, Yuyu Niu, Kui Duan, Jingjing He, Shuchao Ren, Dan Wu, Yun Bai, Zhouchun Shang, Xi Dai, Weizhi Ji &Tianqing Li*. A developmental landscape of 3D-cultured human pre-gastrulation embryos.Nature2019 Dec 12. doi: 10.1038/s41586-019-1875-y
Zongyong Ai; Baohua Niu; Kui Duan; Chenyang Si; Sile Wang; Lifeng Xiang; Xiaoqing Zhu; Qingyuan Zhu; Chun Feng; Yu Yin; Shumei Zhao; Ruize Kong; Weizhi Ji;Tianqing Li*. Modulation of Wnt and Activin/Nodal supports efficient derivation, cloning and suspension expansion of human pluripotent stem cells. 2020in revision.
Li T*, Ai Z, Ji W*. Primate stem cells: bridge the translation from basic research to clinic application. Sci China Life Sci. 2019 Jan; 62(1): 12-21
Yu Kang, Zongyong Ai, Kui Duan, Chengyang Si, Yong Wang, Yun Zheng, Jingjing He, Yin Yu, Shumei Zhao, Baohua Niu, Xiaoqing Zhu, Li Liu, Lifeng Xiang, Linming Zhang, Yuyu Niu, Weizhi Ji,Tianqing Li*. Increased Survival of Primed Pluripotent Stem Cells Injected into Embryos Results in the Generation of Chimeric Monkeys.Cell Reports2018, 25: 2563–2576 (IF, 8.3)
Zhu X, Zhou W, Jin H,Li T*. Brn2 Alone Is Sufficient to Convert Astrocytes into Neural Progenitors and Neurons.Stem Cells Dev.2018 Jun 1;27(11):736-744.
LI Jun, ZHANG Hui-Yun, JIANG Yong,LI Tian-Qing*. Comparison of Parkinson’s monkey models induced by unilateral and bilateral intracerebroventricular injections of MPP+.Acta Physiologica Sinica,2017, December 25, 69(6): 743–750
Yuyu Niu,Tianqing Liand Weizhi Ji*. Paving the road for biomedicine: genome editing and stem cells in primates.National Science Review2017, 4: 543–549
Yongchang Chen, Yuyu Niu, Yanjiao Li, Zongyong Ai, Yu Kang, Hong Shi, Zheng Xiang, Zhaohui Yang, Tao Tan, Wei Si, Wei Li, Xueshan Xia, Qi Zhou, Weizhi Ji, andTianqing Li*. Generation of Cynomolgus Monkey Chimeric Fetuses using Embryonic Stem Cells.Cell Stem Cell2015 Jul 2; 17(1): 116-24 (IF, 23.5)
Bin Jiang, Zheng Xiang, Zongyong Ai, Hong Wang, Yanjiao Li, Weizhi Ji, andTianqing Li*. Generation of cardiac spheres from primate pluripotent stem cells in a small molecule-based 3D system.Biomaterials2015 Oct; 65: 103-14. (IF, 9.3)
Ruofeng Wang,Tianqing Li*. DNA Methylation is Correlated with Pluripotency of Stem Cells.Curr Stem Cell Res Ther.2017; 12(6): 442-446
Xiaoqing Zhu, Bo Li, Zongyong Ai, Kunshang Zhang, Yongchang Chen, Yuemin Li, Hong Wang, Joshua D. Rizak, Xintian Hu, Yi Eve Sun, Weizhi Ji andTianqing Li*. A robust single primate neuroepithelial cell clonal expansion system for disease studies.Stem Cell Reports2016 Feb 9; 6(2): 228-42. (IF, 7.6)
Xiaoqing Zhu, Zongyong Ai, Xintian Hu andTianqing Li*. Efficient Generation of Corticofugal Projection Neurons from Human Embryonic Stem Cells.Scientific Reports2016 June 27 DOI: 10.1038/srep28572.
Jingjing He, Zheng Xiang, Xiaoqing Zhu, Zongyong Ai, Jingsong Shen, Tianzhuang Huang, Liegang Liu, Weizhi Ji &Tianqing Li*. Neuroprotective Effects of 7, 8-dihydroxyflavone on Midbrain Dopaminergic Neurons in MPP+-treated Monkeys.Scientific Reports, 2016 Oct 12; 6: 34339. doi: 10.1038/srep34339.
Sun T, Chen S, Huang H,Li T, Yang W, Liu L. Metabolic profile study of 7, 8-dihydroxyflavone in monkey plasma using high performance liquid chromatography-tandem mass spectrometry.J Chromatogr B Analyt Technol Biomed Life Sci. 2017 Sep 1;1061-1062:97-102.
Yun Zheng, Bo Ji, Renhua Song, Shengpeng Wang, Ting Li, Xiaotuo Zhang, Kun Chen,Tianqing Liand Jinyan Li. Accurate detection for a wide range of mutation and editing sites of MicroRNAs from small RNA high-throughput sequencing profiles.Nucleic Acids Research, 2016 Aug 19;44(14):e123.
Ai Z Y, Zhao SM,Li TQ*. Research on primate naive pluripotent stem cells and its challenges.SCIENTIA SINICA Vitae, 2015, 45: 1203–1213
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Niu Y, Shen B, Cui Y, Chen Y, Wang J, Wang L, Kang Y, Zhao X, Si W, Li W, Xiang AP, Zhou J, Guo X, Bi Y, Si C, Hu B, Dong G, Wang H, Zhou Z,Li T, Tan T, Pu X, Wang F, Ji S, Zhou Q, Huang X, Ji W, Sha J.Generation of Gene-Modified Cynomolgus Monkey via Cas9/RNA-Mediated Gene Targeting in One-Cell Embryos.Cell2014;156:836-843
Liu H, Chen Y, Niu Y, Zhang K, Kang Y, Ge W, LiuX, Zhao E, Wang C, Lin S, Jing B, Si C, Lin Q, Chen X, Lin H, Pu X, Wang Y, Qin B, Wang F, Wang H, Si W, Zhou J, Tan T,Li T, Ji S, Xue Z, Luo Y, Cheng L, Zhou Q, Li S, Sun YE, Ji W.TALEN-Mediated Gene Mutagenesis in Rhesus and Cynomolgus Monkeys.Cell Stem Cell, 2014;14:323-328.
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Xinjie Chen#,Tianqing Li#（共同第一作者）,Xuemei Li#，Yunhua Xie, Xiangyu Guo, Shaohui Ji, Yiyu Niu, Yang Yu, Chenhui Ding, Ruqiang Yao, Shihua Yang, Weizhi Ji, Qi Zhou.Neural Progenitors Derived from monkey ES cells in a simple monoculturesystem.Reprod Biomed Online2009;19(3):426–433
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