朱作言文集（全2冊）

內容簡介

朱作言先生研究團隊於20 世紀80 年代初期戒判籃首創農業動物基因工程研究，在1985 年發表了世界第一篇魚類基因故境埋轉移研究文章，享有世界聲譽。本書從該團隊發表的200 多篇文章中，選取了100 篇編輯成冊，分雅煉尋放上卷和下卷出版。本書的主線是基於轉基因魚理論模型的建立、轉基因鯉研製及品系選育、轉植基因功能，以及轉基因魚食用和生態安全研究。內容共包括7 個部分：第一部分和第二部分建立了轉基因魚研究的完整理論模型，並以此為基礎成功研製轉“全魚”生長激素基因黃河鯉家系，系統揭示了轉再檔海危植基因的整合、表達和遺傳規律。第三部分從攝食、代謝、生長、免疫和游泳行為等方面，詳細描述了轉基因黃河鯉與野生黃河鯉的生物學特性。第四部分系統評估了轉基因魚在實驗室及模擬自然環境條件下的生態學效應。第五部分系統研究了魚類生長激素對性腺發育的調控作用、育性控制技術及研製不育轉基因魚的策略。第六部分包括草魚全基因組草圖繪製、草魚病原病毒分子生全催物學和抗病草魚基因工程育種的基礎研究。第七部分主要以斑馬魚為模型，對魚類基因工程的生物學原理、技術拓展和套用進行了全方位的探討。為了便於廣大讀者閱讀，英文文章後面均附有中文摘危背禁要。

圖書目錄

上卷

第一部分魚類基因轉移模型建立

Novel Gene Transfer into the Fertilized Eggs of Goldfish (Carassius auratus L 1758) 3

人笑寒元生長激素基因在泥鰍受精卵顯微注射轉移後的生物學效應 8

Introduction of Novel Genes into Fish 11

轉基因魚模型的建立 23

第二部分外源基因的整合與表達

The β-Actin Gene of Carp (Ctenopharyngodon idella) 35

Isolation and Characterization of β-Actin Gene of Carp (Cyprinus carpio) 37

Primary-Structural and Evolutionary Analyses of the Growth-Hormone Gene from Grass

Carp (Ctenopharyngodon idellus) 54

Time Course of Foreign Gene Integration and Expression in Transgenic Fish Embryos 64

Transgenes in F4 pMThGH-Transgenic Common Carp (Cyprinus carpio L ) are Highly

Polymorphic 73

Sequences of Transgene Insertion Sites in Transgenic F4 Common Carp 84

Characterization of Transgene Integration Pattern in F4 hGH-Transgenic Common Carp

(Cyprinus carpio L ) 87

Integration of Double-Fluorescence Expression Vectors into Zebrafish Genome for the

Selection of Site-Directed Knockout/Knockin 101

Site-Directed Gene Integration in Transgenic Zebrafish Mediated by Cre Recombinase

Using A Combination of Mutant Lox Sites 114

Inhibition of No Tail (ntl) Gene Expression in Zebrafish by External Guide Sequence

(EGS) Technique 128

Non-Homologous End Joining Plays A Key Role in Transgene Concatemer Formation in

Transgenic Zebrafish Embryos 137

Integration Mechanisms of Transgenes and Population Fitness of GH Transgenic Fish 157

Gene Transfer and Mutagenesis Mediated by Sleeping Beauty Transposon in Nile Tilapia

(Oreochromis niloticus) 172

Targeted Expression in Zebrafish Primordial Germ Cells by Cre/LoxP and Gal4/UAS

Systems 189

Inheritable and Precise Large Genomic Deletions of Non-Coding RNA Genes in

Zebrafish Using TALENs 211

Efficient Ligase 3-Dependent Microhomology-Mediated End Joining Repair of DNA

Double-Strand Breaks in Zebrafish Embryos 225

第三部分外源基因表達的生理功能

Hormonal Replacement Therapy in Fish: Human Growth Hormone Gene Function in

Hypophysectomized Carp 249

Food Consumption and Energy Budget in MThGH-Transgenic F2 Red Carp (Cyprinus

carpio L red var ) 263

Whole-Body Amino Acid Pattern of F4 Human Growth Hormone Gene-Transgenic Red

Common Carp (Cyprinus carpio) Fed Diets with Different Protein Levels 269

Transgene for Growth Hormone in Common Carp (Cyprinus carpio L ) Promotes Thymus

Development 276

Effects of the “All-Fish” Growth Hormone Transgene Expression on Non-Specific

Immune Functions of Common Carp, Cyprinus carpio L 287

Rapid Growth Cost in “All-Fish” Growth Hormone Gene Transgenic Carp: Reduced

Critical Swimming Speed 298

Growth and Energy Budget of F2 ‘All-Fish’ Growth Hormone Gene Transgenic Common

Carp 307

Fast-Growing Transgenic Common Carp Mounting Compensatory Growth 321

Metabolism Traits of ‘All-Fish’ Growth Hormone Transgenic Common Carp (Cyprinus

carpio L ) 334

Enzyme-Linked Immunosorbent Assay of Changes in Serum Levels of Growth

Hormone (cGH) in Common Carps (Cyprinus carpio) 350

The Hematological Response to Exhaustive Exercise in ‘All-Fish’ Growth

Hormone Transgenic Common Carp (Cyprinus carpio L ) 362

Acute and Chronic Un-Ionized Ammonia Toxicity to ‘All-Fish’ Growth Hormone

Transgenic Common Carp (Cyprinus carpio L ) 376

Effects of Growth Hormone (GH) Transgene and Nutrition on Growth and Bone

Development in Common Carp 386

Increased Food Intake in Growth Hormone-Transgenic Common Carp (Cyprinus carpio L )

May Be Mediated by Upregulating Agouti-Related Protein (AgRP) 401

第四部分轉基因黃河鯉的生態安全研究

Progress in the Evaluation of Transgenic Fish for Possible Ecological Risk and Its

Containment Strategies 423

Reduced Swimming Abilities in Fast-Growing Transgenic Common Carp Cyprinus carpio

Associated with Their Morphological Variations 433

Elevated Ability to Compete for Limited Food Resources by ‘All-Fish’ Growth

Hormone Transgenic Common Carp Cyprinus carpio 446

Increased Mortality of Growth-Enhanced Transgenic Common Carp (Cyprinus carpio L )

Under Short-Term Predation Risk 462

Behavioral Alterations in GH Transgenic Common Carp May Explain Enhanced

Competitive Feeding Ability 471

Risk-Taking Behaviour May Explain High Predation Mortality of GH-Transgenic

Common Carp Cyprinus carpio 489

Transgenic Common Carp Do Not Have the Ability to Expand Populations 506

Rapid Growth Increases Intrinsic Predation Risk in Genetically Modified Cyprinus carpio:

Implications for Environmental Risk 518

第五部分魚類生殖調控與轉基因魚的育性控制研究

A Perspective on Fish Gonad Manipulation for Biotechnical Applications 535

Cloning and Expression Analysis in Mature Individuals of Two Chicken Type-II GnRH

(cGnRH-II) Genes in Common Carp (Cyprinus carpio) 546

Identification and Characterization of A Novel Splice Variant of Gonadotropin α

Subunit in the Common Carp Cyprinus carpio 560

Antisense for Gonadotropin-Releasing Hormone Reduces Gonadotropin Synthesis and

Gonadal Development in Transgenic Common Carp (Cyprinus carpio) 574

Production, Characterization, and Applications of Mouse Monoclonal Antibodies Against

Gonadotropin, Somatolactin, and Prolactin from Common Carp (Cyprinus carpio) 588

Defining Global Gene Expression Changes of the Hypothalamic-Pituitary-Gonadal Axis in

Female sGnRH-Antisense Transgenic Common Carp (Cyprinus carpio) 601

Rapid Growth and Sterility of Growth Hormone Gene Transgenic Triploid Carp 625

Progress in Studies of Fish Reproductive Development Regulation 636

Effects of Growth Hormone Over-Expression on Reproduction in the Common Carp

Cyprinus carpio L 655

Endocrinology: Advances Through Omics and Related Technologies 678

A Controllable On-Off Strategy for the Reproductive Containment of Fish 706

The Distribution of Kisspeptin (Kiss)1- and Kiss2-Postive Neurones and Their Connections

with Gonadotrophin-Releasing Hormone-3 Neurones in the Zebrafish Brain 726

Direct Production of XYDMY– Sex Reversal Female Medaka (Oryzias latipes) by

Embryo Microinjection of TALENs 749

下卷

第六部分抗病草魚基因工程技術育種研究

Sequence of Genome Segments 1, 2, and 3 of the Grass Carp Reovirus (Genus

Aquareovirus, Family Reoviridae) 775

Genome Segment S8 of Grass Carp Hemorrhage Virus Encodes A Virion Protein 783

Molecular Characterization and Expression of the M6 Gene of Grass Carp Hemorrhage

Virus (GCHV), An Aquareovirus 789

Complete Nucleotide Sequence of the S10 Genome Segment of Grass Carp Reovirus (GCRV) 796

Introduction of the Human Lactoferrin Gene into Grass Carp (Ctenopharyngodon idellus) to

Increase Resistance Against GCH Virus 806

Enhanced Resistance to Aeromonas hydrophila Infection and Enhanced Phagocytic

Activities in Human Lactoferrin-Transgenic Grass Carp (Ctenopharyngodon idellus) 816

Molecular Cloning, Characterization and Expression Analysis of the PKZ Gene in Rare

Minnow Gobiocypris rarus 827

Toll-Like Receptor 3 Regulates Mx Expression in Rare Minnow Gobiocypris rarus

After Viral Infection 842

Enhanced Grass Carp Reovirus Resistance of Mx-Transgenic Rare Minnow

(Gobiocypris rarus) 861

Isolation and Characterization of Argonaute 2: A Key Gene of the RNA Interference

Pathway in the Rare Minnow, Gobiocypris rarus 877

Transcriptome Analysis of Head Kidney in Grass Carp and Discovery of Immune-Related

Genes 892

Cloning and Characterization of the Grass Carp (Ctenopharyngodon idella) Toll-Like

Receptor 22 Gene, A Fish-Specific Gene 911

Identification, Characterization and the Interaction of Tollip and IRAK-1 in Grass Carp

(Ctenopharyngodon idellus) 930

Isolation and Analysis of A Novel Grass Carp Toll-Like Receptor 4 (tlr4) Gene Cluster

Involved in the Response to Grass Carp Reovirus 950

Cloning and Preliminary Functional Studies of the JAM-A Gene in Grass Carp

(Ctenopharyngodon idellus) 962

RNA-Seq Profiles from Grass Carp Tissues After Reovirus (GCRV) Infection Based on

Singular and Modular Enrichment Analyses 980

Isolation and Expression of Grass Carp Toll-Like Receptor 5a (CiTLR5a) and 5b

(CiTLR5b) Gene Involved in the Response to Flagellin Stimulation and Grass

Carp Reovirus Infection 1001

Characterizations of Four Toll-Like Receptor 4s in Grass Carp Ctenopharyngodon idellus and

Their Response to Grass Carp Reovirus Infection and Lipopolysaccharide Stimulation 1022

The Draft Genome of the Grass Carp (Ctenopharyngodon idellus) Provides Insights into

Its Evolution and Vegetarian Adaptation 1035

第七部分魚類基因轉移育種技術展望

Embryonic and Genetic Manipulation in Fish 1057

Nuclear Transplantation with Early-Embryonic Cells of Transgenic Fish 1067

The Onset of Foreign Gene Transcription in Nuclear-Transferred Embryos of Fish 1075

Nuclear Transplantation of Somatic Cells of Transgenic Red Carp (Cyprinus carpio

haematopterus) 1086

Nuclear Transplantation in Different Strains of Zebrafish 1095

Cytoplasmic Impact on Cross-Genus Cloned Fish Derived from Transgenic Common

Carp (Cyprinus carpio) Nuclei and Goldfish (Carassius auratus) Enucleated Eggs 1103

Identification of Differentially Expressed Genes from the Cross-Subfamily Cloned

Embryos Derived from Zebrafish Nuclei and Rare Minnow Enucleated Eggs 1117

Identification and Characterization of A Novel Gene Differentially Expressed in

Zebrafish Cross-Subfamily Cloned Embryos 1132

Identification of Differential Transcript Profiles Between Mutual Crossbred Embryos of

Zebrafish (Danio rerio) and Chinese Rare Minnow (Gobiocypris rarus) by

cDNA-AFLP 1146

Identification of A Novel Gene K23 Over-Expressed in Fish Cross-Subfamily Cloned

Embryos 1162

Identification of Differentially Expressed Genes Between Cloned and

Zygote-Developing Zebrafish (Danio rerio) Embryos at the Dome Stage

Using Suppression Subtractive Hybridization 1171

Critical Developmental Stages for the Efficiency of Somatic Cell Nuclear Transfer in

Zebrafish 1196

Cross-Species Cloning: Influence of Cytoplasmic Factors on Development 1212

Efficient RNA Interference in Zebrafish Embryos Using siRNA Synthesized with SP6

RNA Polymerase 1219

Cloning, Characterization and Promoter Analysis of Common Carp

Hairy/Enhancer-of-Split-Related Gene, her6 1233

Knock Down of gfp and No Tail Expression in Zebrafish Embryo by In Vivo-Transcribed

Short Hairpin RNA with T7 Plasmid System 1246

Hybrid Cytomegalovirus-U6 Promoter-Based Plasmid Vectors Improve Efficiency of

RNA Interference in Zebrafish 1260

The Cytomegalovirus Promoter-Driven Short Hairpin RNA Constructs Mediate

Effective RNA Interference in Zebrafish In Vivo 1272

Molecular Cloning of Growth Hormone Receptor (GHR) from Common Carp

(Cyprinus carpio L ) and Identification of Its Two Forms of mRNA Transcripts 1286

Comparative Expression Analysis of GHR Signaling Related Factors in Zebrafish

(Danio rerio) and An In Vivo Model to Study GHR Signaling 1298

Activation of GH Signaling and GH-Independent Stimulation of Growth in

Zebrafish by Introduction of A Constitutively Activated GHR Construct 1318

Vitreoscilla Hemoglobin (VHb) Overexpression Increases Hypoxia Tolerance in

Zebrafish (Danio rerio) 1335

Double Transgenesis of Humanized fat1 and fat2 Genes Promotes Omega-3

Polyunsaturated Fatty Acids Synthesis in a Zebrafish Model 1350

Developments in Transgenic Fish in the People’s Republic of China 1374

Father of Biological Cloning in China 1386

Fish Genome Manipulation and Directional Breeding 1389

後記 1403

朱作言文集（全2冊）

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