方顯楊，男，武漢大學本科，中國科學院生物物理研究所博士，美國國立衛生研究院國立癌症研究所博士後，歷任清華大學生命學院助理教授、副教授，現任中國科學院生物物理研究所研究員(三級)、研究組長、博導。
基本介紹
- 中文名：方顯楊
- 國籍：中國
- 畢業院校：武漢大學
- 職稱：教授
教育/工作經歷,研究領域,研究論文,
教育/工作經歷
1998.09 – 2002.07武漢大學 獲理學學士學位 (化學)
2002.09 – 2008.12 中國科學院生物物理研究所 獲理學博士學位 (生物化學與分子生物學)
2009.03 – 2015.01 美國國立衛生研究院 博士後/Research Fellow
2015.04 – 2023.07 清華大學生命科學學院助理教授/副教授，博士生導師
2023.08 – 至今 中國科學院生物物理研究所 研究員、研究組長、博士生導師
研究領域
課題組聚焦於RNA整合結構生物學研究。一方面致力於RNA的體外與原位高級結構研究新方法與新方案的開發，在此基礎上，整合多種方法（包括X射線晶體學、小角X射線散射、核磁共振、電子順磁共振、單分子螢光共振能量轉移、單分子納米孔、結構預測與計算模擬等）開展與人類疾病或重大生命過程密切相關的RNA的高級結構、構象動態、相互作用與功能關係的研究，另一方面致力於靶向RNA與基於RNA的新療法的開發。
課題組目前的主要研究方向為：
1. RNA高級結構研究新方法與新方案的開發
2. 基於多方法整合的長鏈RNA高級結構與功能研究
3. 基於單分子和系綜方法的RNA構象動態與功能研究
4. 靶向RNA與基於RNA的新療法開發
研究論文
1. Kanglong Yang, Guanglin Chen, Fan Yu, Xianyang Fang, Jiahai Zhang, Zhiyong Zhang, Yunyu Shi, Liang Zhang. Molecular mechanism of specific HLA-A mRNA recognition by theRNA-binding-protein hMEX3B to promote tumor immune escape. Communications Biology 2024, 7(1):158
2. Xiaolin Niu,Zhonghe Xu, Yufan Zhang, Xiaobing Zuo, Chunlai Chen, Xianyang Fang. Structural and dynamicmechanisms for coupled folding and tRNA recognition of a translational T-boxriboswitch. Nature Communications 2023, 14(1):7394.
3. Guang-Yuan Song, Xing-YaoHuang, Meng-Jiao He, Hang-Yu Zhou, Rui-Ting Li, Ying Tian, YanWang, Meng-Li Cheng, Xiang Chen, Rong-Rong Zhang, Chao Zhou, Jia Zhou, Xian-Yang Fang, Xiao-Feng Li, Cheng-Feng Qin. A singleamino acid substitution in the capsid protein of Zika virus contributes to aneurovirulent phenotype. Nature Communications 2023, 14(1):68324.
4. Jie Deng, Xianyang Fang, Lin Huang, Shanshan Li, Lilei Xu, Keqiong Ye, Jinsong Zhang, Kaiming Zhang, Qiangfeng Cliff Zhang. RNA structure determination: From 2D to 3D. Fundamental Research 2023, 3 (5): 727-7375.
5. Keyun Huang, Xianyang Fang. A review on recent advances in methods for site-directed spin labeling of long RNAs. International Journal of Biological Macromolecules 2023, 239:124244.
6. XiangChen, YanWang, ZhongheXu, Meng-LiCheng, Qing-QingMa, Rui-Ting Li, Zheng-JianWang, HuiZhao, XiaobingZuo, Xiao-FengLi, Xianyang Fang, Cheng-FengQin.Zika virusRNA structure controls its unique neurotropism by bipartite binding toMusashi-1. Nature Communications 2023,14(1): 1134
7. Lilei Xu, Yu Xiao, Jie Zhang, Xianyang Fang. Structural insights into translationregulation by THF-II riboswitch. Nucleic Acids Research 2023, 51(2):952-965.
8. XianyangFang, Jose Gallego, Yun-Xing Wang.Deriving RNA topological structure from SAXS. Methods in Enzymology2022, 677:479-529.
9.JieZhang, Binxian Chen, Xianyang Fang.3D structural analysis of long non-coding RNAs by SAXS and computationalmodeling. Methods in Molecular Biology 2023,2568:147-163 (Springer Protocol Book Series).
10. Yanping Hu, Yan Wang,Jaideep Singh，Ruirui Sun, Lilei Xu, XiaolinNiu, Keyun Huang, Guangcan Bai, Guoquan Liu, Xiaobing Zuo, Chunlai Chen, PeterZ. Qin, Xianyang Fang. Phosphorothioate-basedposttranscriptional site-specific labeling of large RNAs for structural anddynamic studies. ACS Chemical Biology 2022, 17(9):2448-2460
11. Bingbing Xu,Changchang Cao, Hao Chen, Qiongli Jin,Guangnan Li, Junfeng Ma, Jieyu Zhao,Jianghui Zhu, Yiliang Ding, Xianyang Fang,Yongfeng Jin, Chun Kit Kwok, Aiming Ren,Yue Wan, Zhiye Wang, Yuanchao Xue, HuakunZhang, Qiangfeng Cliff Zhang, Yu Zhou. Recent advancesin RNA structurome.Science China Life Sciences 2022，65(7):1285-1324
12. Burkhard Endeward, Yanping Hu, Guangcan Bai, Guoquan Liu, ThomasF. Prisner,Xianyang Fang.Long-range distance determination in fully deuterated RNA with pulsed EPRspectroscopy. Biophysical Journal 2022，121(1):37-43.
13. Xiaolin Niu,Ruirui Sun, Zhifeng Chen, YirongYao, Xiaobing Zuo, Chunlai Chen, Xianyang Fang. Pseudoknot length modulates the folding,conformational dynamics and robustness of Xrn1 resistance of flaviviral xrRNAs. NatureCommunications 2021, 12(1):6417
14. Junfeng Ma, Xiang Cheng, Zhonghe Xu, YikanZhang, Jaione Valle, Shilong Fan, Xiaobing Zuo, Iñigo Lasa, Xianyang Fang. Structuralmechanism for modulation of functional amyloid and biofilm formation byStaphylococcal Bap protein switch. The EMBO Journal 2021, 40(14): e107500. (Research Highlight on Nature ChemicalBiology 2021, 17: 839)
15. YouqiTao, Jingfei Xie, Qinglu Zhong, YongyaoWang, Shengnan Zhang, FengLuo, Fengcai Wen, Jingjing Xie, Jiawei Zhao, Xiaoou Sun, HoufangLong, Junfeng Ma, Qian Zhang, Jiangang Long, Xianyang Fang, Ying Lu, Dan Li, MingLi, Jidong Zhu, Bo Sun, Guohui Li, JiajieDiao, Cong Liu. A novel partially-open stateof SHP2 points to a "multiple gear" regulation mechanism. Journal of Biological Chemistry 2021, 296: 100538.
16. Jaione Valle, Xianyang Fang, IñigoLasa*. Revisiting Bap multidomain protein: more than sticking bacteria together.Frontiers in Microbiology 2020,11:613581. (Review)
17. Xiaolin Niu, Qiuhan Liu,Zhonghe Xu, Zhifeng Chen, Linghui Xu, Lilei Xu, Jinghong Li*, Xianyang Fang*. Molecular mechanismsunderlying the mechanical anisotropy of flaviviral exoribonuclease-resistantRNAs (xrRNAs). Nature Communications 2020,11(1): 5496. (Newsand Views on Nature Chemical Biology 2021, 17: 933-934)
18. Yan Wang, Venkatesan Kathiresan, Yaoyi Chen,Yanping Hu, Wei Jiang, Guangcan Bai, Guoquan Liu, Peter Z. Qin*, XianyangFang*. Posttranscriptional site-directed spinlabeling of large RNAs with an unnatural base pair system under non-denaturingconditions.Chemical Science 2020, 11: 9655-9664.
19. Yan Wang, Yaoyi Chen, Yanping Hu, Xianyang Fang*. Site-specific covalentlabeling of large RNAs with nanoparticles empowered by expanded geneticalphabet transcription. Proceedings of theNational Academy of Sciences 2020,117(37): 22823-22832.
20. Yinlong Song, Yikan Zhang,Ying Pan, Jianfeng He, Yan Wang, Wei Chen, Jing Guo, Haiteng Deng, Yi Xue*, XianyangFang*, Xin Liang*. The dimeric organization that enhances themicrotubule end-binding affinity of EB1 is susceptible to phosphorylation. Journal of CellScience2020, 133(9): jcs241216.
21. Qiu-Yan Zhang,Xiao-Feng Li,Xiaolin Niu, NaLi, Hong-JiangWang, Cheng-LinDeng, Han-Qing Ye,Xing-Yao Huang, QiChen, Yan-Peng Xu,Hao-Long Dong, Xiao-DanLi, Hui Zhao,Pei-Yong Shi, Zhi-MingYuan, Peng Gong,Xianyang Fang, Cheng-FengQin*, Bo Zhang*. Short Direct Repeats in the 3′ UntranslatedRegion Are Involved in Subgenomic Flaviviral RNA Production. Journal ofVirology 2020，94(6):e01175-19.
22. Mengran Chen,Han Pan, Lingfei Sun,Peng Shi, Yikan Zhang, Le Li, Yuxing Huang, Jianhui Chen, Peng Jiang, XianyangFang, Congying Wu, and Zhucheng Chen*. Structure and regulationof human epithelial cell transforming 2 protein. Proceedingsof the National Academy of Sciences 2020, 117(2)：1027-1035.
23. Yupeng Zhang,Yikan Zhang, Zhong-Yu Liu，Meng-Li Cheng, Junfeng Ma, YanWang, Cheng-Feng Qin*, Xianyang Fang*. Long non-coding subgenomic flavivirus RNAs haveextended 3D structures and are flexible in solution. EMBO Reports 2019, 20(11): e47016.
24. LiliaKaustov, AlexanderLemak, HongWu,Marco Faini, LixinFan, Xianyang Fang,Hong Zeng, ShiliDuan, AbdellahAllali-Hassani, Fengling Li,Yong Wei, MasoudVedadi, RuediAebersold, Yunxing Wang,Scott Houliston, CherylH Arrowsmith*. The MLL1 trimericcatalytic complex is a dynamic conformational ensemble stabilized by multipleweak interactions. Nucleic Acids Research 2019, 47(17): 9433-9447.
25. Jingxia Lu, Jiao Li, Yuan Wu, Xianyang Fang, Jiapeng Zhu*, Huan Wang*. Characterizationof the FMN-Dependent Cysteine Decarboxylase from Thioviridamide Biosynthesis. OrganicLetters 2019, 21(12):4676-4679.
26. YanxiangZhao, Yikan Zhang, Jinguang Huang, Shanshan Wang, Long Yi, Xin Zhang, MinXu, XianyangFang*, Junfeng Liu*. The effect of phosphate ion on the ssDNAbinding mode of MoSub1, a Sub1/PC4 homolog from rice blast fungus. Proteins: Structure, Function, and Genetics 2019, 87(4):257-264.
27. Yan Wang, Jingbo Jiang,Yachao Gao, Yang Sun, Jianfeng Dai, Yang Wu, Di Qu, Gang Ma, Xianyang Fang*. Staphylococcus epidermidis small basic protein (Sbp) forms amyloid fibrils, consistentwith its function as a scaffolding protein in biofilms. Journalof Biological Chemistry 2018, 293(37):14296-14311.
28. SenyanZhang, Panpan Zhou, Pengfei Wang, Yangyang Li, Liwei Jiang, Wenxu Jia, Han Wang,Angela Fan, Dongli Wang, Xuanling Shi, XianyangFang, Michal Hammel,Shuying Wang, Xinquan Wang*, Linqi Zhang*. StructuralDefinition of a Unique Neutralization Epitope on the Receptor-Binding Domain ofMERS-CoV Spike Glycoprotein. Cell Reports 2018, 24(2): 441-452.
29. Yanan Dong, Yajuan Mu, Yongchao Xie,Yupeng Zhang, Youyou Han, Yu Zhou, Wenhe Wang, Zihe Liu, Mei Wu, Hao Wang, Man Pan,Ning Xu, Cong-Qiao Xu, Maojun Yang, Shilong Fan, Haiteng Deng, Tianwei Tan,Xiaoyun Liu, Lei Liu, Jun Li, Jiawei Wang, XianyangFang, Yue Feng*. Structural basis of ubiquitin modification by theLegionella effector SdeA. Nature 2018, 557(7707): 674-678.
30. Yuba R. Bhandari, Lixin Fan, XianyangFang, George F. Zaki, EricA. Stahlberg, Wei Jiang, Charles D.Schwieters, Jason R. Stagno, Yun-XingWang*.Topological Structure Determination of RNA UsingSmall-Angle X-Ray Scattering. Journal of Molecular Biology 2017, 429 (23): 3635-3649.
31. Xianyang Fang*, Malgorzata Michnicka, Yikan Zhang, Yun-Xing Wang, Edward P. Nikonowicz*. Capture and Release of tRNA by the T-LoopReceptor in the Function of the T-Box Riboswitch. Biochemistry 2017, 56(28): 3549-3558.
32. He Song, Xianyang Fang, Lan Jin, Gary X.Shaw,Yun-Xing Wang, Xinhua Ji*. The Functional Cycle of Rnt1p: Five ConsecutiveSteps of Double-Stranded RNA Processing by a Eukaryotic RNase III. Structure2017, 25: 353-363.
33. Xian Xia, Xiaoyu Liu, Tong Li, Xianyang Fang, Zhucheng Chen*. Structure ofChromatin Remodeller Swi2/Snf2 in the Resting State. Nature Structural and Molecular Biology 2016, 23:722-729.
34. Smita Kakar, Xianyang Fang, Lucyna Lubkowska, Yan Ning Zhou, Gary X. Shaw, Yun-Xing Wang,Ding Jun Jin*, Mikhail Kashlev*, Xinhua Ji*. Allosteric Activation ofBacterial Swi2/Snf2 (Switch/Sucrose Non-fermentable) Protein RapA by RNAPolymerase: BIOCHEMICAL AND STRUCTURAL STUDIES. Journal of Biological Chemistry 2015, 290 (39): 23656-69.
35. Robert A. Dick*, Siddhartha A. K. Datta*, HirshNanda, Xianyang Fang, Yi Wen,Marilia Barros, Yun-Xing Wang, Alan Rein, Volker M. Vogt. Hydrodynamic andMembrane Binding Properties of Purified Rous Sarcoma Virus Gag Protein. Journal of Virololgy 2015, 89:10371-10382.
36. Xianyang Fang, Jason R.Stagno, Yuba Bh.andari, Xiaobing Zuo, Yun-Xing Wang*. Small-angle X-rayscattering: a bridge between RNA secondary structures and three-dimensionaltopological structures. Current Opinion in Structural Biology2015, 30: 147-160. (Review)
37. Sujay Subbayya Ithychanda, Xianyang Fang, Maradumane L. Mohan, Liang Zhu, Kalyan C. Tirupula,Sathyamangla V. Naga Prasad, Yun-Xing Wang, Sadashiva Karnik, Jun Qin*. Amechanism of global shape-dependent recognition and phosphorylation of filaminby protein kinase A. Journal of Biological Chemistry 2015, 290(13): 8527-38.
38. Yi Peng, Joseph E. Curtis, Xianyang Fang, Sarah A. Woodson*. Structural Model of the folded rpoS mRNA leader in complex withHfq. Proceedings of the NationalAcademy of Sciences 2014, 111(48): 17134-9.
39. AlexanderLemak, Bin Wu, Adelinda Yee, Scott Houliston, Hsiau-WeiLee, Aleksandras Gutmanas, Xianyang Fang, Maite Garcia, AnthonySemesi, Yun-Xing Wang, James H.Prestegard, Cheryl H. Arrowsmith*. Structuralcharacterization of a flexible two-domain protein in solution based using Small Angle X-ray Scattering and NMR Data.Structure2014, 22(12): 1862-74.
40. Feng-Ming James Chang, Coyne, H. Jerome,Ramirez, Ciro Alberto Cubillas, Pablo Vinuesa Fleischmann,Xianyang Fang, Zhen Ma,Dejian Ma, John D. Helmann, Alejandro García-de losSantos, Yun-XingWang, Charles E. Dann III, David P. Giedroc*. Cu(I)-mediated AllostericSwitching in a Copper-sensing Operon Repressor (CsoR). Journal of Biological Chemistry 2014,289(27): 19204-17.
41. Xianyang Fang, Jinbu Wang,Ina P. O’Carroll, Michelle Mitchell, Xiaobing Zuo, Yi Wang, PingYu, Yu Liu, Jason W. Rausch, Marzena A. Dyba, Jørgen Kjems, Charles D.Schwieters, Soenke Seifert, Randall E. Winans, Norman R. Watts, Stephen J.Stahl, Paul T. Wingfield, R. Andrew Byrd, Stuart F. J. Le Grice, AlanRein*,Yun-Xing Wang*. An unusual topological structure of the HIV-1 revresponse element, Cell 2013, 155(3): 594-605.
42. Moshe Giladi,Itay Friedberg, Xianyang Fang,Reuben Hiller, Yun-Xing Wang, Daniel Khananshvili*. G503 is obligatory for dynamic coupling of regulatory domains in NCXproteins Biochemistry 2012,51(37): 7313-20.
43. Moshe Giladi,Yeheskel Sasson, Xianyang Fang,Reuben Hiller, Tal Buki, Yun-Xing Wang, Joel Alan Hirsch, Daniel Khananshvili*.A common Ca-driven interdomain module governs eukaryotic NCXregulation, PLoS One 2012, 7(6): e39985.
44. George Avvakumov, John Walker, Sheng Xue,Abdellah Allali-Hassani, Abdalin Asinas, Usha Nair, Xianyang Fang, Xiaobing Zuo, Yun-Xing Wang, Keith Wilkinson, SiranoDhe-Paganon*. Two ZnF-UBP domains in isopeptidase T (USP5), Biochemistry2012, 51(6): 1188-98.
45. Xianyang Fang, YingangFeng, Jinfeng Wang*. Favorable contribution of the C-terminal residue K97 tothe stability of a hyperthermophilic archaeal [P62A]Ssh10b, Archivesof Biochemistry and Biophysics 2009, 481(1): 52-8.
46. Xianyang Fang, Qiu Cui,Yufeng Tong, Yingang Feng, Lu Shan, Li Huang, Jinfeng Wang*. A stabilizing a/β-hydrophobic coregreatly contributes to hyperthermostability of archaeal [P62A]Ssh10b. Biochemistry2008, 47(43): 11212-21.
47. Quan Luo, Xianyang Fang, Zhiwei Deng, Jinfeng Wang*. NMR study of influencesof D51N mutation on backbone conformation of hyperthermophilic archaeal[P62A]Ssh10b. Chemical Journal of ChineseUniversities-Chinese 2008, 29: 546-550.
48. Yan-Jun Hu, Yi Liu*, Xue-Song Shen, Xian-Yang Fang, Song-Sheng Qu. Studieson the interaction between 1-hexylcarbamoyl-5-fluorouracil and bovine serumalbumin. Journal of Molecular Structure 2005, 738: 143–147.
