徐坤，男，1985年7月生，遺傳學博士、西北農林科技大學副教授。
基本介紹
- 中文名：徐坤
- 出生日期：1985年7月
- 畢業院校：西北農林科技大學
- 學位/學歷：博士
- 職業：教師
- 專業方向：遺傳說
- 任職院校：西北農林科技大學
- 性別：男
- 職稱：副教授
人物經歷,社會兼職,研究方向,承擔課程,學術成果,科研項目,學術論文,叢書章節論文,發明專利,
人物經歷
2009年6月本科畢業於西北農林科技大學/生命科學學院/生物工程基地班。2015年6月博士畢業於西北農林科技大學/動物科技學院/遺傳學碩博連讀專業。同年留校工作，任職動物科技學院/動物生化與分子生物學教研室講師，歸屬動物科技學院張智英教授/動物基因組編輯科研團隊。2018年1月任職動物科技學院副教授。
社會兼職
第三屆西北農林科技大學博士後聯誼會理事長(2016.01-2017.12)，中國遺傳學會會員。2017年應Frontiers in Genetics雜誌邀請擔任Precise Genome Editing Techniques and Applications專題客座編輯(Topic Editor)。
研究方向
動物生物技術，主要從事動物基因充拜局編輯技術和動物口服酵母營養免疫技術的開發及套用研究。
承擔課程
承擔本科生《分子生物學(含實驗)》、《動物生物化學實茅乘滲局驗》和研究生《動物基因工程技術》等課程教學任務。2018年陝西希套戀首屆高校課堂教學創新大賽三等獎，2018年西北農林科技大學青年教師講課比賽三等獎。
學術成果
累計發表研究論著41篇，其中SCI期刊論文27篇，叢書章節論文2篇；以第一/通訊作者發表SCI論文17篇，累計影響因子70.631（發表當年）。申請發明專利8項，其中已授權1項。近五年，主持轉基因生物新品種培育重點課題子任務、國家自然科學基金、陝西省自然科學基金、中國博士後基金等科研項目8項，累計獲批科研經費117.5萬元。
科研項目
[1] 轉基因生物新品種培育重大專項（重點課題）：CRISPR/Cas9介導的新型雙等位無篩選標記轉基因技術（2018ZX0801009B）--雙等位打靶及“無縫編輯”技術平台的建立子任務，201萬元（拜盛項37萬元），2018.01-2019.12，在研，子任務負責人。
[2] 國家自然科學基金青年項目：CRISPR/Cas9介導HDR-SSA兩步法豬IGF2基因“無縫編輯”新技術研究（31702099），25萬元，2018.01-2020.12，在研，主持。
[3] 陝西省自然科槳陵料充學基礎研究計畫青年項目：基因組精確編輯技術中不同形式供體DNA的最佳化、比較和套用研轎危究（2017JQ3007）鍵捆紙，5萬元，2018.01-2019.12，在研，主持。
[4] 中國博士後科學基金面上項目一等資助：Cas9切口酶和多元RNA陣列介導豬IGF2基因安全高效編輯（2015M580887），8萬元，2016.01-2018.12，在研，主持。
[5] 中國博士後科學基金特別資助：羊絨生長相關lncRNA基因雙等位無篩選標記敲除研究（2018T111111），15萬元，2018.01-2019.12，在研，主持。
學術論文
（同等貢獻，*通訊作者）
SCI期刊論文（動物基因編輯方向）
[1]Sun Yongsen, Yan Nana, Mu Lu, Sun Bing, Deng Jingrong, Fang Yuanyuan,Shao Simin, Yan Qiang, HanFurong, Zhang Zhiying*,Xu Kun*. sgRNA-shRNA structure mediated SNP site editing on porcine IGF2 gene by CRISPR/StCas9. Front Genet. 2019 Apr 18; 10:347.(2018-IF="3.517, 中科院生物類2區)
[2] Ren Chonghua, Xu Kun,David Jay Sega, Zhang Zhiying*. Strategies for the enrichment and selection of genetically modified cells. Trends Biotechnol. 2019Jan;37(1):56-71.(2018-IF=13.747, 中科院工程技術類1區，Top期刊)
[3] Li Xinyi, Bai Yichun, Cheng Xinzhen, Peter Girgis Tawfek Kalds, Sun Bing, Wu Yun, Lyu Huijiao,Xu Kun*, Zhang Zhiying*. Efficient SSA-mediated precise genome editing using CRISPR/Cas9. FEBS J. 2018Sep;285(18):3362-75. (2017-IF="4.530, 中科院生物類2區，Editor’s Choice)
[4] Shao Simin, Ren Chonghua, Liu Zhongtian, Bai Yichun, Chen Zhilong, Wei Zehui, Wang Xin, Zhang Zhiying*, Xu Kun*. Enhancing CRISPR/Cas9-mediated homology directed repair in mammalian cells by expressing Saccharomyces cerevisiae Rad52. Int J Biochem Cell Biol. 2017Nov;92:43-52.(2016-IF=3.505, 中科院生物類2區)
[5] Wu Yun, Xu Kun,Ren Chonghua, Li Xinyi, Lv Huijiao, Han Furong, Wei Zehui, Wang Xin, Zhang Zhiying*. Enhanced CRISPR/Cas9-mediated biallelic genome targeting with dual surrogate reporter-integrated donors. FEBS Lett. 2017Mar;591(6):903-13.(2016-IF="3.623，Highlight Article,發表當年：中科院生物類2區，Top期刊)
[6] Yan Qiang, Xu Kun, Xing Jiani, Zhang Tingting, Wang Xin, Wei Zehui, Ren Chonghua, Liu Zhongtian, Zhang Zhiying*. Multiplex CRISPR/Cas9-based genome engineering enhanced by Drosha-mediated sgRNA-shRNA.Sci Rep. 2016 Dec 12;6:38970.（2015-IF=5.228, 發表當年：中科院綜合性期刊類2區）
[7] Xu Kun, Ren Chonghua, Liu Zhongtian, Zhang Tao, Zhang Tingting, Li Duo, Wang Ling, Yan Qiang, Guo Lijun, Shen Juncen, Zhang Zhiying*. Efficient genome engineering in eukaryotes using Cas9 from Streptococcus thermophilus .Cell Mol Life Sci. 2015Jan;72(2):383-99.(2014-IF="5.808，中科院生物類2區,GMD Key Scientific Article)
[8]Ren Chonghua, Xu Kun, Liu Zhongtian, Shen Juncen, Han Furong, Chen Zhilong, Zhang Zhiying*. Dual-reporter surrogate systems for efficient enrichment of genetically modified cells. Cell Mol Life Sci.2015Jul;72(14):2763-72.(2014-IF="5.808，中科院生物類2區)
[9]Guo Lijun, Xu Kun, Liu Zhiyuan, Zhang Cunfang, Xin Ying, Zhang Zhiying*. Assembled the Streptococcus thermophilus CRISPR array for multiplex DNA targeting . Anal Biochem. 2015Jun 1;478:131-3.(2014-IF="2.219)
[10] Zhang Cunfang, Xu Kun, Hu Linyong, Wang Ling, Zhang Tingting, Ren Chonghua, Zhang Zhiying*. A suicidal zinc finger nuclease expression coupled with a surrogate reporter for efficient genome engineering.Biotechnol Lett. 2015Feb;37(2):299-305. (2014-IF="1.591)
SCI期刊論文（口服酵母營養免疫方向及其他）
[11]Hafiz Muhammad Zakria, Han Baoquan, Yue Feng, Mu Lu, Fang Yuanyuan, Li Xiaoyu, Xu Kun*,Zhang Zhiying*. Significant body mass increase by oral administration of a cascade of shIL21-MSTNyeast-based DNA vaccine in mice.Biomedicine & Pharmacotherapy (In Press). Accepted for publication.(2018-IF=3.743)
[12] HanBaoquan,XuKun*,Liu Zhongtian, Ge Wei, ShaoSimin, LiPengcheng, YanNana, Li Xinyi, ZhangZhiying*.Oral yeast-based DNA vaccine confers effective protection from Aeromonas hydrophila infection on Carassius auratus.Fish Shellfish Immunol. 2019Jan;84:948-54.(2018-IF=3.298, 中科院農林科學類1區，Top期刊)
[13] Luo Zhixin, Wang Shanhe, Jiao Beilei, Yuan Dan, Dai Dongmei, Wang Lixian, Xu Kun*, Wang Xin*. Gene cloning and seamless site-directed mutagenesis using single strand annealing (SSA).Appl Microbiol Biotechnol.2018September 12; 102(23):10119-26. (2017-IF=3.340, 中科院工程技術類2區，Top期刊)
[14] Xu Kun,Zhang Tingting, Guo Lijun, Xin Ying, Zhang Long, Zhang Zhiying*. Alternative methods for the efficient construction of short hairpin RNA expression vectors. Anal Biochem. 2015 Jun 1;478:23-5. (2014-IF="2.219)
[15]Zhang Zhiqiang, Xu Kun, Xin Ying, Zhang Zhiying*. An efficient method for multiple site-directed mutagenesis using type IIs restriction enzymes. Anal Biochem. 2015May 1;476:26-8. (2014-IF="2.219)
[16] Xu Kun,Zhang Tingting, Wang Ling, Zhang Cunfang, Zhang Long, Ma Lixia, Xin Ying, Ren Chonghua, Zhang Zhiqiang, Yan Qiang, Martineau Daniel, Zhang Zhiying*. Walleye dermal sarcoma virus: expression of a full-length clone or the rv-cyclin (orf a) gene is cytopathic to the host and human tumor cells. Mol Biol Rep. 2013Feb;40(2):1451-61. (2012-IF="2.506)
[17] Wang Ling, Xu Kun, Lin Juan,Shao Simin, Zhang Tingting, Xu Huarong, Wei Zehui, Zhang Zhiying*. A novel genetic system based on zinc finger nucleases for the identification of interactions between proteins in vivo. PLoS One. 2013Dec 31;8(12):e85650.(2012-IF="3.730，發表當年：中科院生物類2區Top期刊)
叢書章節論文
[18]Xu Kun,Liu Zhongtian, Zhang Long, Zhang Tingting, Zhang Zhiying. SiRNA In Vivo-Targeted Delivery to Murine Dendritic Cells by Oral Administration of Recombinant Yeast. In: Shum K., Rossi J. (eds) SiRNA Delivery Methods(Chapter 14). Methods in Molecular Biology(2016), vol 1364. Humana Press, New York, NY.
[19] XingJiani,Zhang Cunfang,XuKun,Hu Linyong, WangLing,ZhangTingting,RenChonghua,Zhang Zhiying.An Improved Genome Engineering Method Using Surrogate Reporter-Coupled Suicidal ZFNs. In: Liu J. (eds) Zinc Finger Proteins(Chapter 13). Methods in Molecular Biology(2018), vol 1867. Humana Press, New York, NY.
發明專利
[1] 用於富集CRISPR/Cas9介導的同源重組修復細胞的篩選報告載體及篩選方法。張智英；閆娜娜；孫永森；徐坤；房圓圓；鄧競榮；穆璐。申請號: CN201910244538. 申請日期2019.03.28.
[2] 一種增強基因表達的自激活Gal4/UAS系統表達盒的建立。徐坤；劉玉萬；張智英；閆娜娜；雒志新；韓寶泉；李欣憶；張嘵蘭。申請號201710381327.1. 申請日期2017.05.25.
[3] 基於人工核酸酶的可視化細胞修復效率報告系統及其建立方法. 張智英; 邵斯旻; 徐坤; 韓芙蓉; 沈俊岑; 白義春. 申請號CN201611020358.6. 申請日期2016.11.14.
[4] 基於CRISPR/Cas9的外源基因敲入整合系統及其建立方法和套用. 張智英; 吳芸; 徐坤;白義春; 呂慧嬌。申請號CN201610703246.4. 申請日期2016.08.22.
[5] 一種利用CRISPR/Cas9技術基於SSA修復的基因無縫編輯方法. 張智英; 白義春; 徐坤;魏澤輝; 和林潔; 任充華; 邵斯旻; 吳芸; 劉中天. 申請號CN201610333062.3. 申請日期2016.05.18.
[6] 一種T7 RNA聚合酶介導的CRISPR/Cas9基因編輯系統的構建方法. 張智英; 邢佳妮; 閆強; 徐坤。申請號: CN201610261461.3. 申請日期2016.04.25.
[7] 一種真核細胞III型啟動子表達CRISPR sgRNA的方法及其套用. 張智英; 閆強; 徐坤;邢佳妮; 郭楊; 任充華. 申請號CN201510500937.X.申請日期2015.08.14.
[8] 一種口服重組酵母及其介導的靶向腸道DC呈遞shRNA的套用. 張智英; 張龍; 邵斯旻; 徐坤;李欣憶; 徐華榮。申請號ZL201310647359.3. 申請日期2013.12.02; 授權日期2016.04.13.
[2] Ren Chonghua, Xu Kun,David Jay Sega, Zhang Zhiying*. Strategies for the enrichment and selection of genetically modified cells. Trends Biotechnol. 2019Jan;37(1):56-71.(2018-IF=13.747, 中科院工程技術類1區，Top期刊)
[3] Li Xinyi, Bai Yichun, Cheng Xinzhen, Peter Girgis Tawfek Kalds, Sun Bing, Wu Yun, Lyu Huijiao,Xu Kun*, Zhang Zhiying*. Efficient SSA-mediated precise genome editing using CRISPR/Cas9. FEBS J. 2018Sep;285(18):3362-75. (2017-IF="4.530, 中科院生物類2區，Editor’s Choice)
[4] Shao Simin, Ren Chonghua, Liu Zhongtian, Bai Yichun, Chen Zhilong, Wei Zehui, Wang Xin, Zhang Zhiying*, Xu Kun*. Enhancing CRISPR/Cas9-mediated homology directed repair in mammalian cells by expressing Saccharomyces cerevisiae Rad52. Int J Biochem Cell Biol. 2017Nov;92:43-52.(2016-IF=3.505, 中科院生物類2區)
[5] Wu Yun, Xu Kun,Ren Chonghua, Li Xinyi, Lv Huijiao, Han Furong, Wei Zehui, Wang Xin, Zhang Zhiying*. Enhanced CRISPR/Cas9-mediated biallelic genome targeting with dual surrogate reporter-integrated donors. FEBS Lett. 2017Mar;591(6):903-13.(2016-IF="3.623，Highlight Article,發表當年：中科院生物類2區，Top期刊)
[6] Yan Qiang, Xu Kun, Xing Jiani, Zhang Tingting, Wang Xin, Wei Zehui, Ren Chonghua, Liu Zhongtian, Zhang Zhiying*. Multiplex CRISPR/Cas9-based genome engineering enhanced by Drosha-mediated sgRNA-shRNA.Sci Rep. 2016 Dec 12;6:38970.（2015-IF=5.228, 發表當年：中科院綜合性期刊類2區）
[7] Xu Kun, Ren Chonghua, Liu Zhongtian, Zhang Tao, Zhang Tingting, Li Duo, Wang Ling, Yan Qiang, Guo Lijun, Shen Juncen, Zhang Zhiying*. Efficient genome engineering in eukaryotes using Cas9 from Streptococcus thermophilus .Cell Mol Life Sci. 2015Jan;72(2):383-99.(2014-IF="5.808，中科院生物類2區,GMD Key Scientific Article)
[8]Ren Chonghua, Xu Kun, Liu Zhongtian, Shen Juncen, Han Furong, Chen Zhilong, Zhang Zhiying*. Dual-reporter surrogate systems for efficient enrichment of genetically modified cells. Cell Mol Life Sci.2015Jul;72(14):2763-72.(2014-IF="5.808，中科院生物類2區)
[9]Guo Lijun, Xu Kun, Liu Zhiyuan, Zhang Cunfang, Xin Ying, Zhang Zhiying*. Assembled the Streptococcus thermophilus CRISPR array for multiplex DNA targeting . Anal Biochem. 2015Jun 1;478:131-3.(2014-IF="2.219)
[10] Zhang Cunfang, Xu Kun, Hu Linyong, Wang Ling, Zhang Tingting, Ren Chonghua, Zhang Zhiying*. A suicidal zinc finger nuclease expression coupled with a surrogate reporter for efficient genome engineering.Biotechnol Lett. 2015Feb;37(2):299-305. (2014-IF="1.591)
SCI期刊論文（口服酵母營養免疫方向及其他）
[11]Hafiz Muhammad Zakria, Han Baoquan, Yue Feng, Mu Lu, Fang Yuanyuan, Li Xiaoyu, Xu Kun*,Zhang Zhiying*. Significant body mass increase by oral administration of a cascade of shIL21-MSTNyeast-based DNA vaccine in mice.Biomedicine & Pharmacotherapy (In Press). Accepted for publication.(2018-IF=3.743)
[12] HanBaoquan,XuKun*,Liu Zhongtian, Ge Wei, ShaoSimin, LiPengcheng, YanNana, Li Xinyi, ZhangZhiying*.Oral yeast-based DNA vaccine confers effective protection from Aeromonas hydrophila infection on Carassius auratus.Fish Shellfish Immunol. 2019Jan;84:948-54.(2018-IF=3.298, 中科院農林科學類1區，Top期刊)
[13] Luo Zhixin, Wang Shanhe, Jiao Beilei, Yuan Dan, Dai Dongmei, Wang Lixian, Xu Kun*, Wang Xin*. Gene cloning and seamless site-directed mutagenesis using single strand annealing (SSA).Appl Microbiol Biotechnol.2018September 12; 102(23):10119-26. (2017-IF=3.340, 中科院工程技術類2區，Top期刊)
[14] Xu Kun,Zhang Tingting, Guo Lijun, Xin Ying, Zhang Long, Zhang Zhiying*. Alternative methods for the efficient construction of short hairpin RNA expression vectors. Anal Biochem. 2015 Jun 1;478:23-5. (2014-IF="2.219)
[15]Zhang Zhiqiang, Xu Kun, Xin Ying, Zhang Zhiying*. An efficient method for multiple site-directed mutagenesis using type IIs restriction enzymes. Anal Biochem. 2015May 1;476:26-8. (2014-IF="2.219)
[16] Xu Kun,Zhang Tingting, Wang Ling, Zhang Cunfang, Zhang Long, Ma Lixia, Xin Ying, Ren Chonghua, Zhang Zhiqiang, Yan Qiang, Martineau Daniel, Zhang Zhiying*. Walleye dermal sarcoma virus: expression of a full-length clone or the rv-cyclin (orf a) gene is cytopathic to the host and human tumor cells. Mol Biol Rep. 2013Feb;40(2):1451-61. (2012-IF="2.506)
[17] Wang Ling, Xu Kun, Lin Juan,Shao Simin, Zhang Tingting, Xu Huarong, Wei Zehui, Zhang Zhiying*. A novel genetic system based on zinc finger nucleases for the identification of interactions between proteins in vivo. PLoS One. 2013Dec 31;8(12):e85650.(2012-IF="3.730，發表當年：中科院生物類2區Top期刊)
叢書章節論文
[18]Xu Kun,Liu Zhongtian, Zhang Long, Zhang Tingting, Zhang Zhiying. SiRNA In Vivo-Targeted Delivery to Murine Dendritic Cells by Oral Administration of Recombinant Yeast. In: Shum K., Rossi J. (eds) SiRNA Delivery Methods(Chapter 14). Methods in Molecular Biology(2016), vol 1364. Humana Press, New York, NY.
[19] XingJiani,Zhang Cunfang,XuKun,Hu Linyong, WangLing,ZhangTingting,RenChonghua,Zhang Zhiying.An Improved Genome Engineering Method Using Surrogate Reporter-Coupled Suicidal ZFNs. In: Liu J. (eds) Zinc Finger Proteins(Chapter 13). Methods in Molecular Biology(2018), vol 1867. Humana Press, New York, NY.
發明專利
[1] 用於富集CRISPR/Cas9介導的同源重組修復細胞的篩選報告載體及篩選方法。張智英；閆娜娜；孫永森；徐坤；房圓圓；鄧競榮；穆璐。申請號: CN201910244538. 申請日期2019.03.28.
[2] 一種增強基因表達的自激活Gal4/UAS系統表達盒的建立。徐坤；劉玉萬；張智英；閆娜娜；雒志新；韓寶泉；李欣憶；張嘵蘭。申請號201710381327.1. 申請日期2017.05.25.
[3] 基於人工核酸酶的可視化細胞修復效率報告系統及其建立方法. 張智英; 邵斯旻; 徐坤; 韓芙蓉; 沈俊岑; 白義春. 申請號CN201611020358.6. 申請日期2016.11.14.
[4] 基於CRISPR/Cas9的外源基因敲入整合系統及其建立方法和套用. 張智英; 吳芸; 徐坤;白義春; 呂慧嬌。申請號CN201610703246.4. 申請日期2016.08.22.
[5] 一種利用CRISPR/Cas9技術基於SSA修復的基因無縫編輯方法. 張智英; 白義春; 徐坤;魏澤輝; 和林潔; 任充華; 邵斯旻; 吳芸; 劉中天. 申請號CN201610333062.3. 申請日期2016.05.18.
[6] 一種T7 RNA聚合酶介導的CRISPR/Cas9基因編輯系統的構建方法. 張智英; 邢佳妮; 閆強; 徐坤。申請號: CN201610261461.3. 申請日期2016.04.25.
[7] 一種真核細胞III型啟動子表達CRISPR sgRNA的方法及其套用. 張智英; 閆強; 徐坤;邢佳妮; 郭楊; 任充華. 申請號CN201510500937.X.申請日期2015.08.14.
[8] 一種口服重組酵母及其介導的靶向腸道DC呈遞shRNA的套用. 張智英; 張龍; 邵斯旻; 徐坤;李欣憶; 徐華榮。申請號ZL201310647359.3. 申請日期2013.12.02; 授權日期2016.04.13.
