孫周通，博士，中國科學院天津工業生物技術研究所，研究員。
基本介紹
- 中文名：孫周通
- 國籍：中國
- 畢業院校：河南大學
- 職業：科研工作者
- 主要成就：2014.11, 中國產學研合作創新成果獎
人物簡介,研究方向,代表論著,科研項目,獲獎及榮譽,
人物簡介
1. 教育經歷
2005.9-2011.12, 中國科學院上海生命科學研究院，微生物學，博士
2003.9-2005.6, 河南大學, 生物工程, 工學學士
2000.9-2003.6, 河南牧業經濟學院（原鄭州牧專）, 生物技術
2. 工作經歷
2016.9-至今, 中國科學院天津工業生物技術研究所，研究員
2013.10-2016.9, 德國馬普煤炭研究所/菲利普馬爾堡大學，博士後
2012.10-2013.9, 新加坡南洋理工大學，博士後研究員
2012.1-2012.9, 中國科學院上海生命科學研究院/上海工業生物技術研發中心，助理研究員/項目主管
2008.7-2011.12, 上海工業生物技術研發中心，項目負責人（兼）
2007.4-2008.6, 中國科學院上海生命科學研究院湖州工業生物技術中心，項目負責人（兼）
2007.4-2008.6, 中國科學院上海生命科學研究院湖州工業生物技術中心，項目負責人（兼）
研究方向
酶定向進化與生物催化
酶分子工程是實現微生物合成生物學中酶新功能新反應設計與套用的重要手段；對酶催化口袋的再設計與新反應催化機制的研究是新酶新反應設計中的重要研究內容，是實現醫藥中間體、天然產物等微生物合成的再設計與產物手性控制的理論與套用基礎。課題組圍繞酶定向設計方法的研究與酶底物結合口袋的再設計與催化機制的解析，針對天然生物酶存在諸如催化效率低、立體選擇/區域選擇性較差、底物譜窄、熱穩定性差及催化機制不清楚等問題。基於生物信息學與計算設計進行新型工業用酶挖掘設計與再設計，結合分子定向進化改造及計算機(in silico)輔助設計與篩選；開展重要工業醫藥用酶，如：環氧水解酶、醇脫氫酶、P450單加氧酶、胺基酸脫氫酶、糖基轉移酶、脂肪酶及羧酸還原酶等的研究；開發酶的精準設計與定向進化方法，基於晶體結構與計算解析新反應催化機制，套用於酶催化重要醫藥中間體及天然產物的高效微生物催化合成。
代表論著
論文
2020
33. Chen, J, Fan, F, Qu, G, Tang, J, Xi, Y, Bi, C, Sun Z*, and Zhang, X*. Identification of Absidia orchidis steroid 11β-hydroxylation system and its application in engineering Saccharomyces cerevisiae for one-step biotransformation to produce hydrocortisone. Metab. Eng., 2020, 57, 31-42.
2019
32. 張錕，曲戈，劉衛東，孫周通. 工業酶結構與功能的構效關係. 生物工程學報(Chin. J. Biotech.), 2019, 35(10): 1806-1818.
31. Qu G, Liu B, Zhang K, Jiang Y, Zhai C, Guo J, Wang R, Miao Y, Sun Z*. Computer-assisted engineering of the catalytic activity of a carboxylic acid reductase. J. Biotechnol., 2019, 306, 97-104
30. 曲戈，朱彤，蔣迎迎，吳邊，孫周通*. 蛋白質工程：從定向進化到計算設計. 生物工程學報 (Chin. J. Biotech.), 2019, 35(10), 1843-1856.
29. Li A, Qu G, Sun Z*, and Reetz MT*. Statistical Analysis of the Benefits of Focused Saturation Mutagenesis in Directed Evolution Based on Reduced Amino Acid Alphabets. ACS Catal., 2019, 9, 7769-7778.
28.Qu G, Li A, Acevedo-Rocha CG, Sun Z*, and Reetz MT*. The Crucial Role of Methodology Development in Directed Evolution of Selective Enzymes. Angew. Chem. Int. Ed., 2019, DOI: 10.1002/anie.201901491.
27. Liu B, Qu G, Li J, Fan W, Ma JA, Xu Y, Nie Y*, and Sun Z*. Conformational Dynamics-Guided Loop Engineering of an Alcohol Dehydrogenase: Capture, Turnover and Enantioselective Transformation of Difficult-to-Reduce Ketones. Adv. Synth. Catal., 2019, 361, 3182-3190.
26. Qu G, Liu B, Jiang Y, Nie Y, Yu H, and Sun Z*. Laboratory evolution of an alcohol dehydrogenase towards enantioselective reduction of difficult-to-reduce ketones. Bioresour. Bioprocess. 2019, 6(1):18.
25. Sun Z*, Liu Q, Qu G, Feng Y*, and Reetz MT*. The Utility of B-Factors in Protein Science: Interpreting Rigidity, Flexibility and Internal Motion and Engineering Thermostability. Chem. Rev., 2019, 119, 1626-1665.
24. Qu G, Fu M, Zhao L, Liu B, Liu P, Fan W, Ma J, and Sun Z*. Computational Insights into the Catalytic Mechanism of Bacterial Carboxylic Acid Reductase. J. Chem. Inf. Model., 2019, 59, 832-841.
23. Dai Z, Liu Y, Sun Z, Wang D, Qu G, Ma X, Fan F, Zhang L, Li S, and Zhang X*. Identification of a novel cytochrome P450 enzyme that catalyzes the C-2α hydroxylation of pentacyclic triterpenoids and its application in yeast cell factories. Metab. Eng., 2019, 51, 70-78.
22. 曲戈，張錕，蔣迎迎，孫周通*. 2018諾貝爾化學獎：酶定向進化與噬菌體展示技術. 生物學雜誌 (J. Biol.), 2019, 36(1): 1-6. 特約綜述.
21. Sun Z, and Reetz MT*. CHAPTER 12 Controlling the Regio- and Stereoselectivity of Cytochrome P450 Monooxygenases by Protein Engineering. In Dioxygen-dependent Heme Enzymes, The Royal Society of Chemistry: 2019; pp 274-291.
20. Qu G, Sun Z*, and Reetz MT*. Iterative Saturation Mutagenesis for Semi-rational Enzyme Designin “Protein engineering”, Wiley 2019, Book chapter (in production)
2018
19. Li A, Sun Z, and Reetz MT*. Solid-Phase Gene Synthesis for Mutant Library Construction: The Future of Directed Evolution? ChemBioChem, 2018, 19 (19), 2023-2032.
18. Qu G, Guo J, Yang D, and Sun Z*. Biocatalysis of carboxylic acid reductases: Phylogenesis, Catalytic Mechanism and Potential Applications. Green Chem., 2018, 20(4), 777-792.
17. Qu G, Lonsdale R, Yao P, Li G, Liu B, Reetz MT*, and Sun Z*. Methodology Development in Directed Evolution: Exploring Options When Applying Triple Code Saturation Mutagenesis. ChemBioChem, 2018, 19, 239-246.
16. Sun Z, Wu L, Bocola M, Chan H.C. S, Lonsdale R, Kong X.-D, Yuan S*, Zhou J*, and Reetz MT*. Structural and Computational Insight into the Catalytic Mechanism of Limonene Epoxide Hydrolase Mutants in Stereoselective Transformations. J. Am. Chem. Soc., 2018, 140 (1), 310-318.
15. 曲戈，趙晶，鄭平，孫際賓，孫周通*. 定向進化技術的最新進展. 生物工程學報(Chin. J. Biotech.), 2018, 34(1): 1-11. 特邀綜述。
14. Li A, Acevedo-Rocha CG, Sun Z, Cox T, Xu J, and Reetz MT*. Beating Bias in Directed Evolution of Proteins: Combining High-Fidelity On-Chip Solid-Phase Gene Synthesis with Efficient Gene Assembly for Combinatorial Library Construction. ChemBioChem, 2018, 19(3), 221-228.
13. Acevedo-Rocha CG*,Sun Z*, and Reetz MT*. Clarifying the Difference between Iterative Saturation Mutagenesis as a Rational Guide in Directed Evolution and OmniChange as a Gene Mutagenesis Technique. ChemBioChem, 2018, 19 (24), 2542-2544.
12. Yang J , Zhu Y, Qu G, Zeng Y, Tian C, Dong C, Men Y, Dai L, Sun Z*, Sun Y* and Ma Y. Biosynthesis of dendroketose from different carbon sources using in vitro and in vivo metabolic engineering strategies. Biotechnol. Biofuels, 2018, 11, 290.
Before 2016
11. Sun Z#, Salas PT#,Siirola E#,Lonsdale R#, and Reetz MT*. Exploring productive sequence space in directed evolution using binary patterning versus conventional mutagenesis strategies. Bioresour. Bioprocess, 2016, 3:44, 1-8.
10．Li A, Ilie A, Sun Z, Lonsdale R, Xu JH，and Reetz MT*. Whole-Cell-Catalyzed Multiple Regio- and Stereoselective Functionalizations in Cascade Reactions Enabled by Directed Evolution. Angew. Chem. Int. Ed., 2016, 55, 12026 -12029.
9. Sun Z#, Li G#, Ilie A#, and Reetz MT*. Exploring the substrate scope of mutants derived from the robust alcohol dehydrogenase TbSADH. Tetrahedron Letters, 2016, 57, 3648-3651.
8.Sun Z, Lonsdale R, Li G, and Reetz MT*. Comparing Different Strategies in Directed Evolution of Enzyme Stereoselectivity: Single- versus Double-Code Saturation Mutagenesis. ChemBioChem, 2016, 17, 1865-1872.
7. Li G#, Zhang H#, Sun Z, Liu X*, and Reetz MT*. Multiparameter Optimization in Directed Evolution: Engineering Thermostability, Enantioselectivity and Activity of an Epoxide Hydrolase. ACS Catal., 2016, 6, 3679–3687.
6. Sun Z, Wikmark Y, B？ckvall J-E*, and Reetz MT*. New Concepts for Increasing the Efficiency in Directed Evolution of Stereoselective Enzymes. Chem. Eur. J., 2016, 22, 5046-5054.
5. Sun Z, Lonsdale R, Ilie A, Li G, Zhou J, and Reetz MT*. Catalytic Asymmetric Reduction of Difficult-to-Reduce Ketones: Triple Code Saturation Mutagenesis of an Alcohol Dehydrogenase. ACS Catal., 2016, 6, 1598-1605.
4. Sun Z, Lonsdale R, Wu L, Li G, Li A, Wang J, Zhou J*, and Reetz MT*. Structure-Guided Triple-Code Saturation Mutagenesis: Efficient Tuning of the Stereoselectivity of an Epoxide Hydrolase. ACS Catal., 2016, 6, 1590-1597.
3. Sun Z, Ilie A, and Reetz MT*. Towards the Production of Universal Blood by Structure-guided Directed Evolution of Glycoside Hydrolases. Angew. Chem. Int. Ed., 2015, 54, 9158-9160.
2. Sun Z, Lonsdale R, Kong XD, Xu JH, Zhou J*, and Reetz MT*. Reshaping an Enzyme Binding Pocket for Enhanced and Inverted Stereoselectivity: Use of Smallest Amino Acid Alphabets in Directed Evolution. Angew. Chem. Int. Ed., 2015, 54, 12410-12415.
1.Sun Z, Ning Y, Liu L, Liu Y, Sun B, Jiang W, Yang C, and Yang S*. Metabolic engineering of the L-phenylalanine pathway in Escherichia coli for the production of S- or R-mandelic acid. Microbial Cell Factories, 2011, 10:71. “highly accessed”
申請及授權發明專利
[11]. 孫周通、王紅月、劉貝貝：一種手性胺醇化合物的合成方法。CN201911069262.2
[10]. 孫周通、張錕、梅澤龍、塗然、曲戈：一種高通量篩選酮類化合物的檢測方法及其在酶篩選中的套用。CN201910763316.9
[9]. 孫周通、劉貝貝、曲戈、劉保艷：醇脫氫酶突變體及其在手性雙芳基醇化合物合成中的套用。CN201811252380.2
[8]. 孫周通、趙強、劉保艷、劉貝貝、閆豪傑：一種手性3-氨基-1-丁醇的合成方法。CN201810552521.6
[7]. 孫周通、趙強、劉保艷、曲戈：一種手性2-氨基-1-丁醇的合成方法。CN201711181396.4
[6]. 孫周通、劉保艷、劉貝貝、曲戈：一種手性雙芳基醇化合物的合成方法。CN201711200541.9
[5]. 孫周通、範文超：酶法製備手性3-羥基四氫呋喃的工藝及醇脫氫酶突變體。專利CN201510572955.9.
[4]. 楊晟、陶榮盛、朱傅贇、趙麗麗、蔣宇、楊俊傑、孫周通、沈正權、黃鶴、孫梁棟、董楓、劉映淼：用於生產L-2-氨基丁酸的載體、工程菌株及方法。 專利授權號：ZL201210015308.4. 授權日期：2015-11-18.
[3]. 孫周通、劉映淼、孫兵兵、王禕、吳輝、楊俊傑、黃鶴、楊晟、李成玉、於麗、王德輝：L-色氨酸基因工程菌，其構建方法以及使用其發酵生產L-色氨酸的方法。專利授權號：ZL201010598350.4. 授權日期：2014-8-27.
[2]. 楊晟、孫周通、姜衛紅：手性單體扁桃酸的獲得方法. 專利授權號：ZL201010570887.X. 授權日期：2014-7-16.
[1]. 楊晟、黃鶴、孫周通：一種二氫吡啶二羧酸合成酶。 專利授權號：ZL201010117533.X. 授權日期：2013-10-9.
科研項目
科技部合成生物學重點研發計畫
中國科學院戰略生物資源服務網路計畫生物資源衍生庫-酶庫
中國科學院重點部署項目
國家自然科學基金青年科學基金項目
國家自然科學基金面上項目
天津市自然科學基金面上項目
獲獎及榮譽
2014.11, 中國產學研合作創新成果獎
