吳英亮：武漢大學生命科學學院教授、博士生導師研究方向：（1）蛋白質-蛋白質作用的分子機制； (2) 生物分子信息與工程；(3)多肽藥物篩選與設計。
基本介紹
- 中文名：吳英亮
- 國籍：中華人民共和國
- 畢業院校：武漢大學化學系
- 學位/學歷：博士、研究生
- 專業方向：蛋白質相互作用分子機制、
學習簡歷,工作簡歷,主要學術兼職,主要研究領域,教學情況,承擔科研項目,
學習簡歷
1999.9-2002.7 武漢大學化學系 博士研究生
1996.9-1999.7 南昌大學化學系 碩士研究生
1992.9-1996.7 南昌大學化學系 本科
1996.9-1999.7 南昌大學化學系 碩士研究生
1992.9-1996.7 南昌大學化學系 本科
工作簡歷
2009.11-至今 武漢大學生命科學學院，教授、博士生導師
2008.9-2009.10 武漢大學生命科學學院，副教授、博士生導師
2007.10-2008.9 加拿大Ambrilia生物製藥公司，多肽藥物設計諮詢專家
2004.11-2008.8 武漢大學生命科學學院 副教授、碩士生導師
2002.9-2004.10 武漢大學生命科學學院 博士後
主要學術兼職
2013.11-至今 中國毒理學會生物毒素毒理專業委員會副主任委員
2009.11-2013.10 中國毒理學會生物毒素毒理專業委員會秘書長
2009.11-2013.10 中國毒理學會生物毒素毒理專業委員會秘書長
主要研究領域
主要研究領域為蛋白質相互作用的分子機制、多肽分子工程及多肽藥物發現研究。
主要研究興趣為作用細胞膜離子通道藥用蠍活性多肽的基礎與套用基礎研究。毒蠍在地球上生存進化了4億多年，在我國具有2000多年的藥用史。蠍的主要藥用成份為蠍尾毒液中數量眾多且結構功能多樣的蠍活性多肽（又稱為“蠍毒素”或“蠍毒素多肽”），這些多肽通過選擇性地作用動物和人細胞膜不同種類膜離子通道蛋白及其它受體蛋白，從而具有抗病毒、抗菌、抗類風濕性關節炎、治療多發性硬化症等藥理功能。通過系統深入研究蠍活性肽的結構功能關係及其與靶標蛋白質的作用機制，建立了蠍活性肽與靶標蛋白相互作用的“人工控制識別”新分子工程技術，篩選設計與鑑定具有抗病毒、抗耐藥菌、抗類風濕性關節炎功能等重要藥用前景的蠍活性肽，推進我國特有蠍活性多肽的生物醫藥套用。
主要研究興趣為作用細胞膜離子通道藥用蠍活性多肽的基礎與套用基礎研究。毒蠍在地球上生存進化了4億多年，在我國具有2000多年的藥用史。蠍的主要藥用成份為蠍尾毒液中數量眾多且結構功能多樣的蠍活性多肽（又稱為“蠍毒素”或“蠍毒素多肽”），這些多肽通過選擇性地作用動物和人細胞膜不同種類膜離子通道蛋白及其它受體蛋白，從而具有抗病毒、抗菌、抗類風濕性關節炎、治療多發性硬化症等藥理功能。通過系統深入研究蠍活性肽的結構功能關係及其與靶標蛋白質的作用機制，建立了蠍活性肽與靶標蛋白相互作用的“人工控制識別”新分子工程技術，篩選設計與鑑定具有抗病毒、抗耐藥菌、抗類風濕性關節炎功能等重要藥用前景的蠍活性肽，推進我國特有蠍活性多肽的生物醫藥套用。
教學情況
生物信息學，36學時，授課對象為本科生和研究生。
承擔科研項目
先後承擔了武漢市科技項目、湖北省傑出青年基金項目、教育部新世紀人才基金項目、國家自然科學基金項目、國家重大多肽新藥研發課題、國家科技部973藥物篩選課題等。
代表性研究論文（*為通訊作者）
1.Zongyun Chen, Youtian Hu, Jing Hong, Jun Hu, Weishan Yang, Fang Xiang, Fan Yang, Zili Xie, Zhijian Cao, Wenxin Li, Donghai Lin*, Yingliang Wu*. Toxin acidic residue evolution function-guided design of de novo peptide drugs for immunotherapeutic target Kv1.3 channel. Scientific Reports, 2015 (in press)
1.Zongyun Chen, Youtian Hu, Jing Hong, Jun Hu, Weishan Yang, Fang Xiang, Fan Yang, Zili Xie, Zhijian Cao, Wenxin Li, Donghai Lin*, Yingliang Wu*. Toxin acidic residue evolution function-guided design of de novo peptide drugs for immunotherapeutic target Kv1.3 channel. Scientific Reports, 2015 (in press)
2.Jing Feng, Weishan Yang, Zili Xie, Fang Xiang, Zhijian Cao, Wenxin Li Hongzhen Hu, Zongyun Chen*, Yingliang Wu*. Kv channel S1-S2 linker working as a binding site of human β-defensin 2 for channel activation modulation. J Biol Chem, 2015 (in press)
3.Weishan Yang, Jing Feng, Fang Xiang, Zili Xie, Guoyi Zhang, Jean-Marc Sabatier, Zhijian Cao, Wenxin Li, Zongyun Chen*, Yingliang Wu*. Endogenous animal toxin-like human β-defensin 2 inhibits own K channels through interaction with channel extracellular pore region. Cellular and Molecular Life Science, 2015, 72: 845-853
4.Fang Xiang, Zili Xie, Weishan Yang, Jing Feng, Zhijian Cao, Wenxin Li, Zongyun Chen*, Yingliang Wu*. Plectasin, first animal toxin-like fungal defensing blocking potassium channels through recognizing channel pore region. Toxins, 2015, 7: 34-42
5.Lingna Xu, Tian Li, Honglian Liu, Fan Yang, Songping Liang, Zhijian Cao, Wenxin Li, Yingliang Wu*. Functional characterization of two novel scorpion sodium channel toxins from Lychas mucronatus. Toxicon, 2014, 90: 318-325
6.Zongyun Chen, Youtian Hu, Jun Hu, Weishan Yang, Jean-Marc Sabatier, Michel De Waard, Zhijian Cao, Wenxin Li, Song Han*, Yingliang Wu*. Unusual binding mode of scorpion toxin BmKTX onto potassium channels relies on its distribution of acidic residues. Biochem Biophys Res Commun, 2014, 447(1): 70-76
7.You-Tian Hu, Jun Hu, Tian Li, Jingjing Wei, Jing Feng, Yimei Du, Zhijian Cao, Wenxin Li*, Yingliang Wu*. Open Conformation of hERG Channel Turrets Revealed by a Specific Scorpion Toxin BmKKx2. Cell & Bioscience, 2014, 4: 18
8.Panpan Hou, Rong Zhang, Yongfeng Liu, Jing Feng, Wei Wang, Yingliang Wu and Jiuping Ding. Physiological Role of Kv1.3 Channel in T Lymphocyte Cell Investigated Quantitatively by Kinetic Modeling. PLoS ONE, 2014, 9(3): e89975
9.Tian Li, Lingna Xu, Honglian Liu, Yawen He, Wenxin Li*, Yingliang Wu*. Characterization of a novel BmaTX47 toxin modulating sodium channels: crucial role of expression vectors in toxin pharmacological activity. Toxins, 2014,6: 816-829
10.Cao ZJ, Di ZY, Wu YL*, Li WX*. Overview of scorpion species and their toxins from China. Toxins, 2014, 6: 796-815
11.Chang Xie, Tian Li,Lingna Xu,Congya Yu,Zhijian Cao,Wenxin Li*, Yingliang Wu*.Kv1.3 potassium channel-blocking toxin Ctri9577, novel gating modifier of Kv4.3 potassium channel from the scorpion toxin family. Biochem Biophys Res Commun, 2014, 444(3): 406-410
12.Jian Ma, Youtian Hu, Mingxiong Guo, Zan Huang, Wenxin Li*, Yingliang Wu*. hERG Potassium Channel Blockage by Scorpion Toxin BmKKx2 Enhances Erythroid Differentiation of Human Leukemia Cells K562. PLoS ONE, 2013, 8(12): e84903
13.Cao Z, Yu Y,Wu Y, Hao P, Di Z, He Y, Chen Z, Yang W, Shen Z, He X, Sheng J, Xu X, Pan B, Feng J, Yang X, Hong W, Zhao W, Li Z, Huang K, Li T, Kong Y, Liu H, Jiang D, Zhang B, Hu J, Hu Y, Wang B, Dai J, Yuan B, Feng Y, Huang W, Xing X, Zhao G, Li X*, Li Y*, Li W*. The genome of Mesobuthus martensii reveals a unique adaptation model of arthropods. Nat Commun, 2013, 4: 2602 (並列第一作者)
14.Jun Hu, Su Qiu, Fan Yang, Zhijian Cao, Wenxin Li, Yingliang Wu*. Unique mechanism of the interaction between honey bee toxin TPNQ and rKir1.1 potassium channel explored by computational simulations: Insights into the relative insensitivity of channel towards animal toxins. PLoS ONE, 2013, 8(7): e67213
15.Jing Feng, Youtian Hu, Hong Yi, Shijin Yin, Song Han, Jun Hu, Zongyun Chen, Weishan Yang, Zhijian Cao, Michel De Waard, Jean-Marc Sabatier, Wenxin Li, Yingliang Wu*. Two Conserved Arginine Residues from the SK3 Potassium Channel Outer Vestibule Controls Selectivity of Recognition by Scorpion Toxins. J Biol Chem, 2013, 288(18): 12544-12553
16.Zongyun Chen, Fan Luo, Jing Feng, Weishan Yang, Danyun Zeng, Ruiming Zhao, Zhijian Cao, Maili Liu, Wenxin Li*, Ling Jiang*, Yingliang Wu*. Genomic and Structural Characterization of Kunitz-type Peptide LmKTT-1a Highlights Diversity and Evolution of Scorpion Potassium Channel Toxins. PLoS ONE, 2013, 8(4): e60201
17.Chen Zongyun, Wang Bin, Hu Jun, Yang Weishan, Cao Zhijian, Zhuo Ruiming, Li Wenxin*, Wu Yingliang*. SjAPI, the First Functionally Characterized Ascaris-Type Protease Inhibitor from Animal Venoms. PLoS ONE. 2013, 8(3): e57529
18.Zhi Li, Wan-Hong Liu, Song Han, Bi-Wen Peng, Jun Yin, Ying-Liang Wu*, Xiao-Hua He* and Wen-Xin Li. Selective inhibition of CCR TEM cells activation by a novel peptide targeting Kv1.3 in a rat EAE model. J Biol Chem, 2012, 287: 29479-29494
19.Zhenhuan Zhao, Wei Hong, Zhengyang Zeng, Yingliang Wu, Kanghong Hu, Xiaohui Tian, Wenxin Li* and Zhijian Cao,* Mucroporin-M1 inhibits hepatitis B virus replication by activating the MAPK pathway and down-regulating HNF4α in vitro and in vivo. J Biol Chem, 2012, 287: 30181-30190
20.ZY Chen, DY Zeng, YT Hu, YW He, N Pan, JP Ding, ZJ Cao, ML Liu, WX Li, H Yi*, L Jiang*, YL Wu*. Structural and Functional Diversity of Acidic Scorpion Potassium Channel Toxins,PLoS ONE, 2012, 7(4): e35154
21.Zong-Yun Chen, You-Tian Hu, Wei-Shan Yang, Ya-Wen He, Jing Feng, Bin-Wang, Rui-Ming Zhao, Jiu-Ping Ding, Zhi-Jian Cao, Wen-Xin Li*, Ying-Liang Wu*. Hg1, Novel Peptide Inhibitor Specific for Kv1.3 Channels from First Scorpion Kunitz-type Potassium Channel Toxin Family. J Biol Chem, 2012, 287: 13813-13821
22.Hui Dai, Shijin Yin, Tian Li, Zhijian Cao, Yonghua Ji, Yingliang Wu*, Wenxin Li*. Recombinant expression, purification, and characterization of scorpion toxin BmαTX14. Protein Expression and Purification, 2012, 82: 325-331
23.Su Qiu, Hong Yi, Jun Hu, Zhijian Cao, Yingliang Wu* and Wenxin Li*. The Binding Mode of Fusion Inhibitor T20 onto HIV-1 gp41 and Relevant T20-Resistant Mechanisms Explored by Computational Study. Current HIV Research, 2012, 10(2): 182-194
24.Zheng Fan, Luyang Cao, Yawen He, Jun Hu, Zhiyong Di, Yingliang Wu, Wenxin Li,* and Zhijian Cao*. Ctriporin, a New Anti-Methicillin-Resistant Staphylococcus aureus Peptide from the Venom of the Scorpion Chaerilus tricostatus. Antimicrobial Agents and Chemotherapy, 2011, 55(11): 5220-5229
25.Li Q, Zhao Z, Zhou D, Chen Y, Hong W, Cao L, Yang J, Zhang Y, Shi W, Cao Z, Wu Y, Yan H*, Li W*. Virucidal activity of a scorpion venom peptide variant mucroporin-M1 against measles, SARS-CoV and influenza H5N1 viruses. Peptides, 2011, 32(7): 1518-1525
26.Han, Song; Yin, Shijin; Yi, Hong; Mouhat, Stéphanie; Qiu, Su; Cao, Zhijian; Sabatier, Jean-Marc; Wu, Yingliang*; Li, Wenxin*. Protein-Protein Recognition Control by Modulating Electrostatic Interactions.Journal of Proteome Research, 2010, 9: 3118-3125
27.Ying Wu, Yu Xiong, Sheng Wang, Hong Yi, Hui Li, Na Pan, Frank T Horrigan, Yingliang Wu*, Jiuping Ding*. Inter-subunit coupling in the Pore of BK channels, Journal of Biological Chemistry, 2009, 284(35): 23353-23363
28.Su Qiu, Hong Yi, Hui Liu, Zhijian Cao, Yingliang Wu*, Wenxin Li*. Molecular Information of Charybdotoxin Blockade in the Large Conductance Calcium-activated Potassium Channel. Journal of Chemical Information and Modeling, 2009, 49(7): 1831-1838
29.Shijin Yin, Ling Jiang, Hong Yi, Song Han, Daiwen Yang, Maili Liu, Hui Liu, Zhijian Cao, Yingliang Wu*, Wenxin Li*. Different residues in channel turret determining the selectivity of ADWX-1 inhibitor peptide between Kv1.1 and Kv1.3 channels. Journal of Proteome Research, 2008,7(11): 4890-4897
30.Geliang Gan, Hong Yi, Maorong Chen, Liang Sun, Wenxin Li, Yingliang Wu* and Jiuping Ding*. Structural basis for toxin-resistance of b4 associated calcium-activated potassium (BK) channels. Journal of Biological Chemistry, 2008, 283: 24177-24184
31.Song Han, Hong Yi, Shi-Jin Yin, Zong-Yun Chen, Hui Liu, Zhi-Jian Cao, Ying-Liang Wu*, and Wen-Xin Li*. Structural Basis of a Potent Peptide Inhibitor Designed for Kv1.3 Channel, a Therapeutic Target of Autoimmune Disease. Journal of Biological Chemistry, 2008, 283: 19058-19065
32.Yi H, Qiu S, Cao ZJ, Wu YL*, Li WX*. Molecular basis of inhibitory peptide maurotoxin recognizing Kv1.2 channel explored by ZDOCK and molecular dynamic simulations. PROTEINS: Structure, Function, and Bioinformatics, 2008, 70: 744-54
33.Jin L*, Wu Y*. Molecular Mechanism of the Sea Anemone Toxin ShK Recognizing the Kv1.3 Channel Explored by Docking and Molecular Dynamic Simulations. Journal of Chemical Information and Modeling, 2007, 47: 1967-1972
34.Hong Yi, Zhijian Cao, Shijin Yin, Chao Dai,Yingliang Wu*, Wenxin Li*. Interaction Simulation of hERG K Channel with its specific Bekm-1 peptide: Insights into the Selectivity of Molecular Recognition. Journal of Proteome Research, 2007,6: 611-620
35.Yingliang Wu, Hong Yi, Zhijian Cao, Wenxin Li. Conformation of Trimeric Envelope Glycoproteins: The CD4-dependent Membrane Fusion Mechanism of HIV-1. Journal of Biomolecular Structure & Dynamics, 2007, 25: 1-9
36.Yingliang Wu, Zhijian Cao, Hong Yi, Dahe Jiang, Xin Mao, Hui Liu and Wenxin Li. Simulation of the interaction between ScyTx and small conductance calcium-activated potassium channel by docking and MM-PBSA. Biophysical Journal, 2004, 87(1): 105-112
37.Wu, YL; Hu, B*; Jiang, ZC; Chen, SZ. Low temperature vaporization for ICP-AES determination of palladium in geological samples using sample introduction of gaseous palladium oxinate. Journal of Analytical Atomic Spectrometry, 2002, 17 (2): 121-124
38. Wu, YL; Hu, B; Peng, TY; Jiang, ZC*. Low-temperature volatilization of Be acetylacetonate for sample introduction in ETV-ICP-AES. Analytical Chimica Acta, 2001, 439 (1): 153-158
