南昌小鏈孢菌

基本信息

編號 AMS-91-06

拉丁菌名 Dipariforma H.L.Zhou, Y.G.Zhou et C.S.Xiao gen. nov.

中文菌名 南昌小鏈孢菌

定名人 余名侖

Nomencla Zhou Huiling, Zhou Yuguang, and Xiao Changsong

發表文章 小鏈孢菌屬的一個新種

Article A New Gwnus of Budding and Appendaged Bacteria

作者信息

作者 余名侖

Author Zhou Huiling, Zhou Yuguang, and Xiao Changsong

作者單位 江西大學生物系, 南昌 330047

摘要

小鏈孢菌屬 (Microstreptospora) 是放線菌目 (Actinomycetales) 的新屬[1]. 至今僅發表過一個種. 我們於1983年分離到 一株放線菌, 經鑑定認為是該屬的一個新種.

Abstract A Strain of oxidative Gram negative bacteria, No.4088, was isolated from the root of Sparina anglica grown at the beach of Rudong County, Jiangsu Province.The cells of the strain No.4088 are rod or ovoid shaped, 0.8-1.0 by 1.1-1.5 micrometer, with capsule, non-motile. The grenules of poly-B-hydroxybutyrateand yellow pigment of B-carotin type are produced. Strain No.4088 multiplies with two forms of budding.The one is buds formed primarily by swelling of the prosthecae (hyphae). The other is with yeast-like form; about 20% buds sprout from mother cells directly.These two types of budding may appear on the same cell Strain No.4088 is an organism with respiratory metabolism, never fermentative, aerobic, microaerophilicunder combined nitrogen-free codition. Chemoorganotrophilic, no acid is produced from sugars and alcohols. Glucose, xylose, maltose, acetate, citrate,succinate, malate, a-ketoglutarate, malonate, arginine and casein are utilized as the sole carbon and energy sources. Methanol, formate, propinate, butyrate,iso-butyrate, valerate, iso-valerate, caprate, fumarate, huppurate, aminoacetate, proline, 2,3-butylene glycol, or betaine is no utilized. Oxidase and catalaseare positive. MR and VP are negative. Hydrogen sulfide is not produced. Nitrate is reduced. Litmus milk becomes alkaline. Esculin, starch or gelatinis not hydrolized. Lysine decarboxylase and arginine decarboxylase are positive and ornithine decarboxylase, DNase, and lipase, urease, arginedihydrolase, phenylalanine deaminase are negative. Free nitrogen can be fixed. Lron and manganese can be deposited. Growth occurs in medium containingNaCl not more than 1.5%. Growth temperature: 20-35'C, 30'C is optimum. Growth pH 6.0-9.0, optimum is ph7.5. Guanine and cytidine contents in DNA is 70.1-70.2 mol%.

Budding and appendaged bacteria multiply with three types: binnary,fission, budding at tips of prostecae (hyphae) or budding from the mother cell directly.But no genus posseses both tips of multiplication as strain No.4088, which buds as at tips of posthecae and from mother cell directly.

Pedomicrobium buds at the tips or laterally at the posthecae. Daughter cell are flagellated.

Hyphomonas buds are produced at tips of polar prosthecae (hyphae). Prosthecae are nonseparate and arrely branch under normal growth conditions.Motile by means of a single polar or lateral flagellum located on developing buds or young daughter cell. Amino acids are required.

Hyphomicrobium daughter cell formation by a buuding process at one prothecae, mature buds become motile as swarmers. Poly-B-hydrobutyrateis stored by most cells usually at a district cell pole. The prothecae are not separate.

We propose a new genus name: Dipareforma gen. nov., and a new species Dipareforma spartinacae sp. nov., for bacterium No.4088. The culture isdeposited in Type Culture Collection, Institute of Microbiology, Academia Sinica, with a catalogue number AS 1.1762.

關鍵字 小鏈孢菌屬, 南昌小鏈孢菌

Key

期刊 微生物學報 32(1-6):75-78, 1992

Publication Acta Microbiologica Sinica (Chinese; English Abstract), 31(1-6):pp.413-419, 1991

分享省市 江西省南昌市

Place Rudong County, Jiangsu Province, China

採集地 東郊北羅村休閒的稻 田土

Environment The bacterium isolated in the root of Sparina anglica and the grass collected from the Yellow Sea seabeach at Juedong Reclamation Area.

海撥高度

生態環境 中亞熱帶濕潤氣候水稻田

寄主 稻田土

Habitat subtropical moist monsoon climate grass seabeach

Host the root of Sparina anglica

採集人 余名侖

Isolation Person Zhou Huiling

採集時間

保存單位 江西大學生物系, 南昌 330047

Preservation Unit China General Microorganisms Collection Center, Institute of Microbiology, the Chinese Academy of Sciences, Beijing 100080, China

描述

等模式

Speciman No.

全文

Note The bacterium fixed nitrogen for Sparina anglica, it is a formous forage grass and tolerence salt and alkane in soil.

備註

該菌對枯草桿菌有較弱的抑制作用