匡漢暉

匡漢暉，男，1967年生於江西泰和。現為華中農業大學園藝林學學院蔬菜系教授，

博士生導師。2008年受聘為長江學者特聘教授，

基本介紹

• 中文名：匡漢暉
• 國籍：中國
• 出生地：江西泰和
• 出生日期：1967年

個人履歷

園藝植物生物學教育部重點實驗室常務副主任。

1984-1988年 江西農業大學學習，獲學士學位

1988-1991年 北京林業大學學習，獲碩士學位

1994-1998年在美國喬治亞大學(University of Georgia)學習，獲博士學位

1998-2007年分別在以色列海法大學(University of Haifa)、美國加州大學戴維斯分校(University of California, Davis)和伯克萊分校(University of California, Berkeley)從事蔬菜抗病研究工作

2008年受聘為長江學者特聘教授，現為華中農業大學園藝林學學院蔬菜系教授，博士生導師

主要研究領域及成果

研究領域

植物抗病與分子進化

研究內容

茄科植物的抗病基因。

內容包括抗病基因（NBS-LRR）的生物信息學、遺傳定位及克隆、分子進化、比較基因組學、功能基因組學。　1．基因組學：利用生物信息學方法分析菸草、西紅柿和土豆基因組中的所有NBS-LRR基因。對這些抗病基因進行遺傳定位、比較基因組學和分子進化的研究。利用實驗手段了解抗病基因進化的規律。

2．抗病基因快速克隆方法的建立：通過對抗病基因的序列分析，找出抗病基因的保守序列，設計幾個人工微RNA用以沉默大部分NBS-LRR基因，同時設計一套VIGS沉默載體沉默基因組中的絕大多數抗病基因。這套分析系統將極大提高植物抗性的遺傳鑑定，加快抗病基因的克隆，尤其是QTL抗病基因和抗蟲基因的克隆。這套系統將首先在菸草中進行，試驗成功後再推廣到其它蔬菜和農作物上。我們同時利用第二代高通量測序技術來快速定位和克隆基因。以上技術成熟後將套用到番茄青枯病和黃化曲葉病的抗性資源篩選和抗性基因克隆中。

3．菸草與TMV的相互作用。菸草屬對TMV抗性的進化；不同野生菸草對TMV抗性的分子機理；用實驗的方法了解抗病基因N的突變規律；TMV接種後無症狀性狀的遺傳和分子機制。

代表性論文

1．Luo， S., Peng, J., Li, K., Wang, M., Kuang, H.（2011）Contrasting evolutionary patterns of the Rp1 resistance gene family in different species of Poaceae. Mol. Bio. Evol.　 28: 313-325

2．Kuang, H. Padmanabhan, C., Li, F. , Kamei, A., Bhaskar, P. B., Ouyang, S., Jiang, J., Buell, C. R., and Baker B. (2009) Identification of miniature inverted-repeat transposable elements (MITEs) and biogenesis of their siRNAs in the Solanaceae: New functional implications for MITEs. Genome Research, 19: 42-56.

3．Kuang, H., J. van Eck，H., Sicard，D., Michelmore, R. and Nevo, E. (2008) Evolution and genetic population structure of prickly lettuce (Lactuca serriola) and its RGC2 resistance gene cluster. Genetics, 178: 1547-1558.

4．Kuang, H., Caldwell, K., Meyers, B. and Michelmore, R. (2008) Frequent sequence exchanges between Homologs of RPP8 in Arabidopsis are not necessarily associated with genomic proximity. Plant Journal, 54: 69-80.

5．Zhang, L., Mojtahedi, H., Kuang, H., Baker, B. and Brown, C.R. (2007) The use of STS markers in the marker-assisted selection of Columbia root-knot nematode resistance introgressed from Solanum bulbocastanum. Crop Science, 47: 2021-2026

6．Kuang, H., Ochoa, O., Nevo E., and Michelmore R. (2006) The resistance gene Dm3 is infrequent in natural populations of Lactuca serriola due to deletions and frequent gene conversions. Plant Journal, 47: 38-48

7．Kuang, H., F. Wei, M. Marano, U. Wirtz, X. Wang, J. Liu, W. Shum , J. Zaborsky, L. J. Tallon, W. Rensink, S. Lobst, P. Zhang, C. Tornqvist, A. Tek, J. Bamberg, J. Helgeson, W. Fry, F. You, M. Luo, J. Jiang, R. C. Buell and B. Baker. (2005) The R1 resistance cluster contains three groups of independently evolving, Type I R1 homologues and shows substantial structural variation among haplotypes of Solanum demissum. Plant Journal, 44: 37-51

8．Huang, S., van der Vossen, E.A.G, Kuang, H., Vleeshouwers, V.G.A.A., Zhang, N., Borm, T.J.A., van Eck, H.J., Baker, B., Jacobsen, E. and Visser, R.G.F. (2005) Comparative genomics enabled the isolation of the R3a late blight resistance gene in potato. Plant Journal, 42: 251-261

9．Kuang, H., Woo, S., Meyers, B., Nevo, E., Michelmore, R. (2004) Multiple genetic processes result in heterogeneous rates of evolution within the major cluster disease resistance genes in lettuce. Plant Cell, 16: 2870-2894.

10．Meyers, B., Kozik, A., Griego, A., Kuang, H., R.W. Michelmore (2003). Genome-wide analysis of NBS-LRR-encoding genes in Arabidopsis. Plant Cell 15: 809-834.

11．Song, J., Bradeen, J.M., Naess, S.K., Raasch, J.A.,Haberlach, G.T., Wielgus S.M.,Liu, J., Kuang, H., Austin-Phillips, S., Buell, C.B., Helgeson, J.P., and Jiang, J. (2003) Gene RB from Solanum bulbocastanum confers broad spectrum resistance against potato late blight pathogen Phytophthora infestans. PNAS 100: 9128-9133.

12．Kuang, H., Richardson, T., Carson, P. and Bongarten, B. (1999) Genetic analysis of inbreeding depression in Pinus radiata D. Don. II. Genetics of viability genes. Theor. Appl. Genet. 99: 140-146.

13．Kuang, H., Richardson, T., Carson, S., Wilcox, P. and Bongarten, B. (1999) Genetic analysis of inbreeding depression in Pinus radiata D. Don. I. Genetic map with distorted markers. Theor. Appl. Genet. 98: 697-703.

14．Kuang, H., Rrchardson, T., Carson, S., and Bongarten, B. (1998) An allele responsible for seeding death in Pinus radiata D. Don. Theor. Appl. Genet. 96: 640-644.