侯健(中國農業大學副教授)

侯健，男，博士，中國農業大學動物學與動物生理學系副教授、博士生導師。

基本介紹

• 中文名：侯健
• 畢業院校：中國農業大學
• 學位/學歷：博士
• 專業方向：生理學
• 任職院校：中國農業大學

人物經歷

2006.1至今：中國農業大學生物學院，副教授

2001.7-2005.12：中國農業大學生物學院，講師

1998.9-2001.6：中國農業大學生物學院生理學專業，博士研究生

1995.9-1998.6：內蒙古農業大學動物遺傳與繁育專業，碩士研究生

1991.9-1995.6：內蒙古農業大學畜牧專業，本科

研究方向

動物胚胎髮育與表觀遺傳學，包括卵泡發育、卵子成熟、受精和胚胎髮育等過程中的遺傳學與表觀遺傳學調控機制研究。

動物胚胎工程技術，包括同期發情、超數排卵、體外受精、胚胎冷凍、胚胎移植、胚胎克隆、基因編輯等技術的研究與開發套用。

主講課程

《基因打靶》（本科生）

學術成果

發表論文

1. Wang Q, Zhang Y, Zhong X, Li J, An X, Hou J. Dimethylated histone H3 lysine 9 is dispensable for the interaction between developmental pluripotency-associated protein 3 (Dppa3) and ten-eleven translocation 3 (Tet3) in somatic cells. Reproduction, Fertility and Development, 2018, online.
2. Zhang Y, Gao E, Wang Q, Tian H, Hou J.Effects of histone methyltransferase inhibitor chaetocin on histone H3K9 methylation of cultured ovine somatic cells and development of preimplantation cloned embryos. Reproductive Toxicology, 2018, online.
3. Zhang Y, Wang Q, Liu K, Gao E, Guan H, Hou J.Treatment of donor cells with recombinant KDM4D protein improves preimplantation development of cloned ovine embryos. Cytotechnology, 2018, online.
4. Zhong X, Wang Q, LI J, Zhang Y, An X, Hou J.Ten-eleven translocation-2 (Tet2) is involved in myogenic differentiation of skeletal myoblast cellsin Vitro. Scientific Reports. 2017,7, 43539
5. Zhang N, Li J, Zhong X, An X, Hou J.Reversible immortalization of sheep fetal fibroblast cells by tetracycline-inducible expression of human telomerase reverse transcriptase. Biotechnology Letters, 2016, 38:1261-1268.
6. Fu L, Yan F, An X, Hou J. Effects of the histone methyltransferase inhibitor UNC0638 on histone H3K9 dimethylation of cultured ovine somatic cells and development of resulting early cloned embryos. Reproduction in Domestic Animals, 2014, 49: e21-e25.
7. Fu L, Zhang J, Yan F, Guan H, An X, Hou J. Abnormal histone H3K9 dimethylation but normal dimethyltransferase EHMT2 expression in cloned sheep embryos. Theriogenology, 2012, 78:1929-1938.
8. Li Q, Hou J, Wang S, Chen Y, An X. Production of transgenic rabbit embryos through intracytoplasmic sperm injection. Zygote, 2010, 18:301-307.
9. Li Q, Hou J, Chen Y, An X. Full term development of rabbit embryos produced by ICSI with sperm frozen in liquid nitrogen without cryoprotectants. Reproduction in Domestic Animal, 2010, 45:717-722.
10. Li Q, Hou J, Wang S, Guan H, Zhang N, Chen Y, An X. Viable rabbits derived from oocytes by intracytoplasmic injection of spermatozoa from an infertile male. Zygote, 2009,17:157-162.
11. Hou J, Liu L, Zhang J, Cui XH, Yan F, Guan H, Chen Y, An X. Epigenetic modification of histone 3 at lysine 9 in sheep zygotes and its relationship with DNA methylation. BMC Developmental Biology 2008, 8:60.
12. Liu L, Hou J, Lei T, Bai J, Guan H, An X. Aberrant DNA methylation in cloned ovine embryos. Chinese Science Bulletin, 2008, 53: 477-480.
13. Bai J, Hou J, Guan H, Yan F, Cui X, Liu L, Wang S, An X. Effect of 2-mercaptoethanol and cysteine supplementation during in vitro maturation on the developmental competence of oocytes from hormone-stimulated lambs. Theriogenology, 2008, 70: 758-764.
14. Cui W, Ren L, Hou J, Zhang Y, Chen Y, An X. A model study: simulation of marker gene deletion and target gene replacement mediated by Cre/lox system in cell line. Progress in Biochemistry and Biophysics, 2008, 35: 650-660.
15. Li Q, Guan H, Hou J, An X, Chen Y. Transfer of ovine embryos through a simplified mini-laparotomy technique. Journal of Animal Science, 2008, 86:3224-3227.
16. Hou J, Liu L, Lei T, Cui X, An X, Chen Y. Genomic DNA methylation patterns in bovine preimplantation embryos derived from in vitro fertilization. Sciences in China (Series C), 2007, 50:56-61.
17. Hou J, Lei T, Liu L, Cui X, An X, Chen Y. Demecolcine-induced enucleation of sheep meiotically maturing oocyes. Reproduction, Nutrition and Development. 2006, 46: 219－226.
18. Hou J, Lei T, Liu L, Cui X, An X, Chen Y. DNA methylation patterns in in vitro-fertilised goat zygotes. Reproduction, Fertility and Development. 2005, 17: 809-813.
19. Hou J, Cui X, Lei T, Liu L, An X, Chen Y. Aberrant DNA methylation patterns in cultured mouse embryos. Progress in Natural Science. 2005, 15:1079－1083.
20. Gou K, An X, Guan H, Yang X, Hou J, Lin A, Zeng S, Chen Y. Transgenic twin lambs cloned by granulose cells. Cloning and Stem Cells. 2003,5:71-78.

論著教材

《生物技術概論》，副主編，中國農業大學出版社，2010年第一版

發明專利

1.一種誘導動物體外細胞可逆永生化的載體及其套用，專利號201310692377.3

2.一種用於誘導表達鋅指核酸酶的載體及其套用，專利號201310692299.7

獲獎記錄

1. 國家現代農業產業技術體系建設項目, 2016-2020，在研，主持
2. 畜禽繁殖調控新技術研發,國家重點研發計畫項目，2017-2020，在研，任務主持
3. 轉座子介導安全高效基因編輯技術研究，轉基因新品種培育重大專項，2018-2019，在研，任務主持
4. 主要轉基因動物和飼料安全性檢測及安全控制關鍵技術引進，農業部948計畫項目，2016-2018，在研，任務主持.
5. 羥甲基胞嘧啶及TET蛋白在早期胚胎DNA去甲基化過程中的作用機制研究，國家自然科學基金面上項目，2012-2015，已結題，主持。
6. 可逆型長壽細胞構建及用於綿羊體細胞基因打靶的研究，國家自然科學基金面上項目2011-2013，已結題，主持。
7. DNA甲基化酶和組蛋白甲基化酶在綿羊體細胞克隆胚胎中的表達研究，國家自然科學基金青年基金項目，2008-2010，已結題，主持。
8. 轉基因豬、牛和羊的環境安全評價技術，轉基因新品種培育重大專項：,2011-2013，已結題，參加。
9. 轉基因肉羊新品種培育，轉基因新品種培育重大專項，2008-2010，已結題，子任務主持。