《馬鏈球菌獸疫亞種突破豬腦微血管內皮細胞的分子機制》是范紅結為項目負責人，南京農業大學為依託單位的面上項目。
基本介紹
- 中文名：馬鏈球菌獸疫亞種突破豬腦微血管內皮細胞的分子機制
- 項目類別 ：面上項目
- 依託單位 ：南京農業大學
- 項目負責人：范紅結
科研成果,項目摘要,
科研成果
序號 | 標題 | 類型 | 作者 |
|---|---|---|---|
1 | A novel vaccine against Porcine circovirus type 2 (PCV2) and Streptococcus equi ssp. zooepidemicus (SEZ) co-infection | 期刊論文 | Ma, Zhe|Yang, Xu-qiu|Fan, Hong-jie|Lu, Cheng-ping| |
2 | Identification of novel genes expressed during host infection in Streptococcus equi ssp zooepidemicus ATCC35246 | 期刊論文 | Xu, Bin|Ma, Fang|Peng, Jie|Fan, Hongjie| |
3 | Identification of genes transcribed by Streptococcus equi ssp zooepidemicus in infected porcine lung | 期刊論文 | Xie, Huaidong|Yang, Yongchun|Lu, Chengping|Fan, Hongjie| |
4 | 馬鏈球菌獸疫亞種類M蛋白抗體間接ELISA檢測方法的建立及套用 | 期刊論文 | 周紅|周瑾|范紅結|陸承平| |
5 | Biofilm Formation of Streptococcus equi ssp. zooepidemicus and Comparative Proteomic Analysis of Biofilm and Planktonic Cells | 期刊論文 | Zheng, Jun-xi|Yang, Yong-chun|Fan, Hong-jie|Lu, Cheng-ping| |
6 | SILAC and LC-MS/MS identification of Streptococcus equi ssp zooepidemicus proteins that contribute to mouse brain microvascular endothelial cell infection | 期刊論文 | Pei Xiaomeng|Lin Huixing|Lu Chengping|Fan Hongjie| |
7 | Extracellular Nucleases of Streptococcus equi subsp. zooepidemicus Degrade Neutrophil Extracellular Traps and Impair Macrophage Activity of the Host. | 期刊論文 | Ma, Fang|Guo, Xiao|Fan, Hongjie| |
8 | Virulent and vaccine strains of Streptococcus equi ssp. zooepidemicus have different influences on phagocytosis and cytokine secretion of macrophages. | 期刊論文 | Lin, Huixing|Zhang, Hui|Lu, Chengping|Fan, Hongjie| |
9 | Contribution of fibronectin-binding protein to pathogenesis of Streptococcus equi ssp zooepidemicus | 期刊論文 | Zheng, Jun-xi|Yang, Yong-chun|Lu, Cheng-ping|Fan, Hong-jie| |
10 | A novel suicide shuttle plasmid for Streptococcus suis serotype 2 and Streptococcus equi ssp zooepidemicus gene mutation | 期刊論文 | Zhang, Hui|Yu, Lei|Ma, Zhe|Fan, Hongjie| |
11 | The arginine deiminase system facilitates environmental adaptability of Streptococcus equi ssp zooepidemicus through pH adjustment | 期刊論文 | Zhang, Ping|Ma, Zhe|Lin, Huixing|Fan, Hongjie| |
12 | 馬鏈球菌獸疫亞種串聯表達蛋白對小鼠免疫效果的評價 | 期刊論文 | 周紅|李月|李成貴|范紅結| |
13 | Molecular Characterization of Streptococcus agalactiae Isolated from Bovine Mastitis in Eastern China | 期刊論文 | Yi, Li|Ma, Zhe|Fan, Hongjie|Lu, Chengping| |
14 | Capsule of Streptococcus equi subsp zooepidemicus hampers the adherence and invasion of epithelial and endothelial cells and is attenuated during internalization | 期刊論文 | Pei, Xiaomeng|Su, Yiqi|Ma, Zhe|Fan, Hongjie| |
15 | Identification and characterization of a Streptococcus equi ssp zooepidemicus immunogenic GroEL protein involved in biofilm formation | 期刊論文 | Xu, Bin|Grenier, Daniel|Fan, Hong-Jie|Lu, Cheng-Ping| |
16 | Insight into the specific virulence related genes and toxin-antitoxin virulent pathogenicity islands in swine streptococcosis pathogen Streptococcus equi ssp zooepidemicus strain ATCC35246 | 期刊論文 | Li, Yue|Meng, Qingshu|Fan, Hongjie|Hu, Songnian| |
17 | 馬鏈球菌獸疫亞種纖連蛋白結合蛋白的原核表達及其免疫原性的檢測 | 期刊論文 | 周夢曦|蔣子奇|藺輝星|范紅結| |
項目摘要
馬鏈球菌獸疫亞種（SEZ）舊稱獸疫鏈球菌，是豬鏈球菌病的主要病原。其可引起豬的腦炎，但機制不明。本研究以SEZ強毒菌株ATCC35246為研究對象，利用轉座子誘變技術，建立轉座子突變體庫，以豬腦微血管內皮細胞（PBMEC）為血腦屏障模型，篩選SEZ與突破血腦屏障模型相關的毒力因子，發現粘附蛋白，並通過小鼠模型評價其功能；構建PBMEC文庫，利用酵母雙雜交技術，從文庫中篩選與SEZ突破血腦屏障相關毒力因子的互作蛋白，並進行鑑定，尋找相關粘附受體；同時通過SEZ對PBMEC的侵襲實驗，評價SEZ產生的溶血素、鏈激酶和PBMEC金屬蛋白酶表達量的變化對血腦屏障模型通透性的影響。在此基礎上，採用細胞信號通路發現晶片分析蛋白互作等引起PBMEC細胞信號激活的途徑。結果通過闡明SEZ突破PBMEC血腦屏障模型的分子機制，能進一步解析SEZ致豬腦炎的機理，並為相應的藥物及疫苗設計提供依據。
