非解朊棲熱菌是棲熱菌屬生物。
基本介紹
- 中文名：非解朊棲熱菌
- 外文名： Clostridium succinogenum Tan & Wang sp. nov.
- 編號 ：AMS-92-02
- 定名人：蔡妙英 楊壽鈞
- 發表文章： 一個產生L-乳酸脫氫酶的嗜熱菌的新種 --- 非解朊棲熱菌
作者信息,摘要,
作者信息
作者 蔡妙英 楊壽鈞 劉俊風 衛 軍
Author Tan Peiying and Wang Dasi
作者單位 中國科學院微生物研究所細菌分類室細菌分類組, 北京 100080
摘要
從廣東 省陽江, 電白和韶關等地55-95'C的溫泉中採集水樣, 經 分離得到21株G-, 不產生芽孢的高溫菌. 它們都能產生 L-乳酸脫氫酶. 根據表觀特徵, DNA的同源性和G+Cmol%等 特徵可歸入棲熱菌屬 (Thermus) . 但對葡萄糖不產酸和 不液化明膠等表型特徵不同於已發表的種. 又根據該群內 的DNA/DNA雜交結果, 有高的同源性 (大多在80%以上) , 而與已發表的種僅有40.5-70.7%的同源性. 結果表明可成 立一獨立的類群, 建議為棲熱菌屬的一個新種, 因具不液 化明膠的特性, 命名為非解朊棲熱菌 (Thermus nonproteolytics Cai & Yang sp. nov.).以菌株 HG-42為模式株, 保藏於中國科學院微生物研究所.
Abstract A new species of obligately anaerobic, sporeforming, thermophilic, cellulolytic, saccharolytic, proteolytic Clostridium is described.Two strains(Hll, H12) of this species are isolated from silage (grass) on milk cow field.
Cells in PY-cellobiose broth culture are Gram-negative rods, 0.3 to 0.6 by 1.5 to 5.5 micrometer, motile and peritrichous, occur singly or in pairs,occasionally in short chains. Long filaments may be seen in older cultures. Spores are oval or round, terminal and subterminal, swell the cell.Sporulation occurs readily in PY-cellobiose broth or cellulose broth after incubation for 24 hours. Free spores are round, have an exosporium and appendages.Deep colonies in PY-cellobiose agar roll tubes are lenticular, watery, yellowish color, 1-2mm in diameter, with entire margins.In PY-cellulose agar roll tubes they are pin-like, less than 1mm in diameter, raised, colorless, translucent, with an irregular margins.After incubation for 5 days, colonies are surrounded by clear zones, representing cellulose hydrolysis, that usually are 3 to 7 mm in diameter.Cultures in PY-cellobiose broth are moderate turbid with a stringy sediment and have a pH of 5.0 afterincubation for 5 days. The optimum temperature for growth is 50-53'C. There is little growth at 37'C, and none at 65'C.The optimum pH for growth is 7.5. No growth in media without fermentable carbohydrates. In a chemically defined medium, growth factors required arebiotin, pyridoxine, vitamin B12, and p-aminobenzoic acid. Fermentation products (per 100 mL of culture) are 1.7-2.4 meq of succinic acid,1.0-1.2 meq of acetic acid, less than 1 meq of ethanol, hydrogen, and carbon dioxide in 1% PY-cellobiose or PY-cellulose medium.The mol % G+C of the DNA is 38.8 (Tm). Other characteristics of the species are given in Table 1.
The above characteristics show that the species differ from the known close species of genus Clostridium, sach asClostridium stercorarium. and other cellulolytic thermophile: Clostridium thermocellum, Clostridium thermolacticum, Clostridium josuiand Clostridium thermocoprial. The name Clostridium succinogenum sp. nov. is proposed for this species, referring to chief product in nonvolatile acidof the species. The type strain (H11) has been deposited in the General Microbiological Center of China Committee of Culture Collection of Microorganism as AS 1.1782.
關鍵字 非解腖棲熱菌, L-乳酸脫氫酶, 鑑定
Key
期刊 微生物學報 32(1-6):233-237, 1992
Publication Acta Microbiologica Sinica (Chinese; English Abstract), 32(1-6):pp.155-159, 1992
分享省市 廣東省電白縣
Place Haidian District, Beijing City, China
採集地 尖崗嶺熱水熱泉區的溫 泉水樣
Environment The bacterium isolated from the grass of fermentating greefeed at dairy.
海撥高度
生態環境 亞熱帶季風氣候55-95'C的溫泉
寄主 溫泉中的水樣
Habitat warm-template semi-moist continental climate grassland
Host the grass of fermentating greefeed
採集人 蔡妙英 楊壽鈞 劉俊風 衛 軍
Isolation Person Tan Peiying et al.
採集時間
保存單位 中國科學院微生物研究所細菌分類室細菌分類組, 北京 100080
Preservation Unit China General Microorganisms Collection Center, Institute of Microbiology, the Chinese Academy of Sciences, Beijing 100080, China
描述
等模式
Speciman No. AS 1.1782
全文
Note (1) Paratype strain H11 deposited in Group of Natural Gas Fermentation, Department of Bacteriological Taxonomy, Institute of Microbiology, the Chinese Academy of Sciences, Beijing 100080, China(2) See: Acta Microbiologica Sinica, 27(3):211-216, 1987; Acta Microbiologica Sinica, 31(6):484-487, 1991, for this bacterium reports.
備註
