《雞艾美耳球蟲侵入部位特異性關鍵分子研究》是李祥瑞為項目負責人，南京農業大學為依託單位的青年科學基金項目。
基本介紹
- 中文名：雞艾美耳球蟲侵入部位特異性關鍵分子研究
- 項目類別 ：面上項目
- 依託單位 ：南京農業大學
- 項目負責人：李祥瑞
科研成果,項目摘要,
科研成果
序號 | 標題 | 類型 | 作者 |
|---|---|---|---|
1 | The Molecular Characterization and Immunity Identification of Microneme 3 of Eimeria acervulina | 期刊論文提白漿 | Zhang, ZhenChao(#); Liu, XinChao; Yang, XinChao; Liu, LianRui; Wang, Shuai; Lu, MingMin; Ehsan, Mohammad; Gadahi, Javaid A.; Song, XiaoKai; Xu, LiXin; Yan, RuoFeng; Li, XiangRui(*) |
2 | The molecular characterization and immune protection of microneme 2 of Eimeria acervulina | 期刊論文 | Zhang, ZhenChao(#); Liu, LianRui; Huang, JingWei; Wang, Shuai; Lu, MingMin; Song, XiaoKai; Xu, LiXin; Yan, RuoFeng; Li, XiangRui(*) |
3 | Evaluation of the persistence, integration, histopathology and environmental release of DNA vaccine encoding Eimeria tenella TA4 and chicken IL-2 | 期刊論文 | Song, Xiaokai(#); Zhang, Zeyang; Liu, Chang; Xu, Lixin; Yan, Ruofeng; Li, Xiangrui(*) |
4 | Partial protection against four species of chicken coccidia induced bymultivalent subunit vaccine | 期刊論文 | Xiaokai Song(#); Yunlu Gao; Lixin Xu; Ruofeng Yan; Xiangrui Li(*) |
5 | Eimeria maxima microneme protein 2 delivered as DNA vaccine andrecombinant protein induces immunity against experimental homogenous challenge, | 期刊論文 | Jingwei Huang(#); Zhenchao Zhang; Menghui Li,; Xiaokai Song; Ruofeng Yan; Lixin Xu; Xiangrui Li(*) |
6 | Identification of common immunodominant antigens of Eimeria tenella, Eimeria acervulina and Eimeria maxima by immunoproteomic analysis | 期只虹殼刊論姜罪民文 | Liu, Lianrui(#); Huang, Xinmei(#); Liu, Jianhua; Li, Wenyu; Ji, Yihong; Tian, Di; Tian, Lu; Yang, Xinchao; Xu, Lixin; Yan, Ruofeng; Li, Xiangrui; Song, Xiaokai |
7 | 一種雞艾美耳球蟲戰主免疫調節和店凳榆型多價多表位DNA疫喇端歸苗 | 專利 | 李祥瑞(#)(*); 宋小凱市翻恥乎; 嚴若峰; 徐立新; 任喆 |
8 | Proteomic analysis of Eimeria acervulina sporozoite proteins interactionwith duodenal epithelial cells by shotgun LC–MS/MS | 期刊論文 | ZhenChao Zhang(#); Shuai Wang; JingWei Huang; LianRui Liu; MingMin Lu; MengHui Li; YuXia Sui,; LiXin Xu; RuoFeng Yan; XiaoKai Song; Xiangrui Li(*) |
9 | Chimeric DNA vaccines encoding Eimeria acervulina macrophage migrationinhibitory factor (E.MIF) induce partial protection against experimental Eimeriainfection | 期刊論文 | Xiaokai Song(#); Ruirui Zhang; Lixin Xu; Ruofeng Yan; Xiangrui Li(*) |
10 | Efficacy of chimeric DNA vaccines encoding Eimeria tenella 5401 andchicken IFN-γ or IL-2 against coccidiosis in chickens | 期刊論文 | Xiaokai Song(#); Xinmei Huang(#); Ruofeng Yan; Lixin Xu; Xiangrui Li(*) |
11 | Immune protection duration and efficacy stability of DNA vaccine encoding Eimeria tenella TA4 and chicken IL-2 against coccidiosis | 期刊論文 | Song, Xiaokai(#); Zhao, Xiaofang; Xu, Lixin; Yan, Ruofeng; Li, Xiangrui(*) |
12 | 一種雞球蟲多價重組蛋白亞單位疫苗及其套用 | 專利 | 李祥瑞(#)(*); 嚴若峰; 徐立新; 宋小凱; 高雲路 |
13 | Protective Efficacy of Coccidial Common Antigen Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) against Challenge with Three Eimeria Species | 期刊論文 | Tian, Lu(#); Li, Wenyu; Huang, Xinmei; Tian, Di; Liu, Jianhua; Yang, Xinchao; Liu, Lianrui; Yan, Ruofeng; Xu, Lixin; Li, Xiangrui; Song, Xiaokai |
14 | Identification and immunogenicity of microneme protein 2 (EbMIC2) of Eimeria brunetti | 期刊論文 | Tran Duc Hoan(#); Zhang, Zhenchao; Huang, Jingwei; Yan, Ruofeng; Song, Xiaokai; Xu, Lixin; Li, Xiangrui(*) |
15 | Immune protection of Microneme 7 (EmMIC7) against Eimeria maxima challengein chickens | 期刊論文 | Jingwei Huang(#); Zhenchao Zhang; Menghui Li; Xiaokai Song; Ruofeng Yan; Lixin Xu; Xiangrui Li(*) |
16 | Identification and Molecular Characterization of Microneme 5 of Eimeria acervulina | 期刊論文 | Zhang, ZhenChao(#); Huang, JingWei; Li, MengHui; Sui, YuXia; Wang, Shuai; Liu, LianRui; Xu, LiXin; Yan, RuoFeng; Song, XiaoKai; Li, XiangRui(*) |
17 | Induction of protective immunity against Eimeria tenella, Eimerianecatrix, Eimeria maxima and Eimeria acervulina infectionsusing multivalent epitope DNA vaccines | 期刊論文 | Xiaokai Song(#); Zhe Ren,; Ruofeng Yan; Lixin Xu; Xiangrui Li(*) |
18 | Construction of Eimeria tenella multi-epitope DNA vaccines andtheir protective efficacies against experimental infection. | 期刊論文 | Xiaokai Song(#); Lixin Xua,; Ruofeng Yan; Xinmei Huang; Xiangrui Li(*) |
項目摘要
雞球蟲病由艾美耳屬的7種球蟲感染引起，分別寄生於十二指腸和小腸上段、中段、下段及盲腸，具有明顯的寄生部位特異性。寄生部位特異性取決於侵入部位特異性，但決定部位特異性的蟲體配體和細胞表面受體尚不完全明了。本項目擬選取不同部位寄生的代表性蟲體，根據蟲體配體與細胞表面受體相結合的原理，提取蟲體子孢子膜蛋白或全蟲可溶性蛋白，與雞不同腸段上皮細胞結合，提取結合後細胞膜蛋白或總蛋白，進行2-D電泳分析，並用子孢子蛋白高免血清識別，選取識別蛋白點，通過質譜分析，確認蟲體配體分子。再觀察配體重組蛋白抗血清阻斷蟲體侵入和配體重組蛋白對攻蟲的免疫保護作用，確定蟲體配體。在此基礎上，用酵母雙雜交確認細胞受體分子，用抗血清被動免疫保護試驗進一步驗證受體功能，並觀察受體在整個腸道的分布，從而確定雞球虫部位特異性的關鍵分子。對闡明雞球虫部位特異性的分子機理有重要意義，對研究頂復門其他蟲體的部位特異性也有重要借鑑意義。
