同濟大學再生醫學系幹細胞研究中心、同濟醫院幹細胞臨床轉化中心教授、博士生導師、源品生物首席技術官
2013年，採用單細胞RNA測序技術揭示了人類和小鼠早期胚胎的遺傳程式，研究成果發表在《Nature》雜誌上，該項研究成果將在幹細胞的純化、分類及臨床治療領域產生深遠的影響，也將為改善人類輔助生殖技術和提高人口出生質量提供幫助。
基本介紹
- 中文名：薛志剛
- 職業：教師
- 研究方向：1. 胚胎早期發育的遺傳學與表觀遺傳學研究2. 幹細胞分化表觀遺傳學機制及在轉化醫學中的套用3. 高通量測序的數據分析和生物信息學
科研業績,代表性論著,
科研業績
2013年，採用單細胞RNA測序技術揭示了人類和小鼠早期胚胎的遺傳程式，研究成果發表在《Nature》雜誌上，該研究利用單細胞表達譜研究的RNA-seq技術，突破了起始樣本量少的瓶頸，對人胚胎早期發育各階段全基因組RNA轉錄譜的系統分析，發現在胚胎髮育早期各階段中存在著父親或母親來源的單等位基因表達差異。同時運用加權基因共表達網路分析（WGCNA）顯示胚胎早期發育各階段中的細胞周期、基因調控、蛋白質翻譯以及代謝通路的轉錄變化是以分步進行的方式按順序發生，並明確了該機制在物種間保守存在，僅在發育特異性和時序上有所差異，證明了哺乳動物早期胚胎髮育進化上的共性。此外，該研究還發現了驅動胚胎早期發育各階段的關鍵候選基因。該項研究成果將在幹細胞的純化、分類及臨床治療領域產生深遠的影響，也將為改善人類輔助生殖技術和提高人口出生質量提供幫助。五年內發表論文20餘篇，作為課題負責人獲得國家自然科學基金資助兩項，多次作為科研骨幹參與國家基金委、科技部973、863、國際合作等科研項目，上海市“曙光計畫”項目資助。
代表性論著
Xue Z*, Huang K, Cai CC, Cai LB, Jiang CY, Feng Y, Liu ZS, Zeng Q, Cheng LM, Sun YE, Liu JY*, Horvath S, Fan G*. Genetic programs in human and mouse early embryos revealed by single-cell RNA sequencing. Nature. 500(7464): 593-597. 2013. (first author and corresponding author). IF: 38.597.
Huang K, Shen Y, Xue Z, Bibikova M, April C, Liu Z, Cheng L, Nagy A, Pellegrini M, Fan JB, Fan G*. A panel of CpG methylation sites distinguishes human embryonic stem cells and induced pluripotent stem cells. Stem Cell Reports. 2(1):36-43. 2013.
Liu H, Chen Y, Niu Y, Zhang K, Kang Y, Ge W, Liu X, Zhao E, Wang C, Lin S, Jing B, Si C, Lin Q, Chen X, Lin H, Pu X, Wang Y, Qin B, Wang F, Wang H, Si W, Zhou J, Tan T, Li T, Ji S, Xue Z, Luo Y, Cheng L, Zhou Q, Li S*, Sun YE*, Ji W*. TALEN-Mediated Gene Mutagenesis in Rhesus and Cynomolgus Monkeys. Cell Stem Cell. 14(3):323-328. 2014.
Chen Y, Huang K, Nakatsu MN, Xue Z*, Deng SX, Fan G*. Identification of novel molecular markers through transcriptomic analysis in human fetal and adult corneal endothelial cells. Hum Mol Genet.22(7):1271-1279. 2013. (corresponding author).
Hu G, Huang K, Yu J, Gopalakrishna-Pillai S, Kong J, Xu H, Liu Z, Zhang K, Xu J, Luo Y, Li S, Sun YE, Iverson LE, Xue Z*, Fan G*. Identification of miRNA signatures during the differentiation of hESCs into retinal piJin L, Wu Z, Xu W, Hu X, Zhang J, Xue Z*, Cheng L*. Identifying gene expression profile of spinal cord injury in rat by bioinformatics strategy. Mol Biol Rep. 2014 Mar 5. [Epub ahead of print]
Liu Z, Jiang R, Yuan S, Wang N, Feng Y, Hu G, Zhu X, Huang K, Ma J, Xu G, Liu Q*, Xue Z*, Fan G*. Integrated Analysis of DNA methylation and RNA transcriptome during in vitro differentiation of human pluripotent stem cells into retinal pigment epithelial cells. PLoS One. 9(3):e91416. 2014.
Wang Q, Zhu XM, Feng Y, Xue Z, Fan G*. Single-cell genomics: An overview. Front. Biol. 8(6): 569-576. 2013.
Ge J, Ju Y, Xue Z, Feng Y, Huang X, Liu H, Zhao S*. Distal C Terminus of CaV1.2 Channels Plays a Crucial Role in the Neural Differentiation of Dental Pulp Stem Cells. PLoS One. 8(11):e81332. 2013.
Wu Z, Huang K, Yu J, Le T, Namihira M, Liu Y, Zhang J, Xue Z, Cheng L*, Fan G*. Dnmt3a regulates both proliferation and differentiation of mouse neural stem cells. J Neurosci Res. 90(10):1883-1891. 2012.
Wang A, Huang K, Shen Y, Xue Z, Cai C, Horvath S, Fan G*. Functional modules distinguish human induced pluripotent stem cells from embryonic stem cells. Stem Cells Dev. 20(11):1937-1950. 2011.
Xue Z, Shi Z, Dong J, Liao T, Wang Y, Sun X, Yan Z, Qian X, Cui Y, Chen J, Liu J*, Fan G*. Evaluation of x-inactivation status and cytogenetic stability of human dermal fibroblasts after long-term culture. Int J Cell Biol. 2010:289653. 2010. (first author)
Hutnick L, Golshani P, Namihira M, Xue Z, Matynia A, Yang X, Silva A, Schweizer F, Fan G*. DNA hypomethylation restricted to the murine forebrain induces cortical degeneration and impairs postnatal neuronal maturation. Hum Mol Genet. 18(15):2875-2888. 2009.
