楊立桃

個人簡歷

近年來，主要針對農業與食品領域的轉基因生物和食品安全評估和檢測開展相關研究工作，如基於系統生物學手段的轉基因生物非預期效應評價、分子特徵分析、轉基因生物標準物質研製、基於核酸和蛋白質水平的食品中有毒有害物質分析新技術、轉基因生物安全資料庫開發、轉基因生物與食品安全檢測國家國際標準制定等。先後主持完成國際和上海市科研項目10 餘項。在農業與食品安全領域SCI 收錄的權威期刊發表多驗牛研究論文40 余篇，其中第一作者和通訊作者SCI 論文35 篇，主要期刊如Anal Chem、J Agric Food Chem、BMC Mol Biol、BMC Bioinformatics、Transgenic Res、Food Chem、J AOAC Int 等；申報國家專利7 項，其船射騙拘中獲授權3 項；制定轉基因生物安全檢測方法國家標準20 餘項；作為主要研究人員的研究成果《主要轉基因農產品定性定量檢測技術研究》獲2004 年上海市科學技術進步一等獎。

研究方向

自2002年以來，主要從事轉基因生物檢測、安全評價和核酸分子檢測技術方面的研究和我國轉基因產品檢測國家標準制定等工作，承擔勸備謎櫃和參加國家863計畫、國家自然基金、臘腳鍵國家973計畫子課題和上海市國際合作等課題10餘項，主要有：

（1）國家自然科學基金委青年科學基金項目《轉基因抗蟲水稻非預期效應系統生物學評價技術研究》；

（2）國家863計畫專項課題《4種動物外來疫病通用探針定量 PCR 檢測技術研究及套用驗組台》；

（3）973子課題《外源基盼霉龍因引發非預期效應的分子基礎》；

（4）上海精喇市國際科技合作基金項目《轉基因水稻檢測技術研究及內標準基因協同驗證》；

上海市標準技術專項2項。

發表論文

1. Meng Y, Liu X, Wang S, Zhang D, Yang LT. Applicability of Plasmid Calibrant pTC1507 in Quantification of TC1507 Maize: An Inter-laboratory Study. J Agric Food Chem. 2012. 60: 23-8. (通訊作者)

2. Guo J, Chen L, Liu X, Gao Y, Zhang D, Yang LT. Multiplex degenerate PCR analytical approach targeting to eight genes for screening GMOs. Food Chem, 2012. 132: 1566-73. (通訊作者)

3. Li X, Pan L, Li J, Zhang Q, Zhang S, Lv R, Yang L. Establishment and application of event-specific polymerase chain reaction methods for two genetically modified soybean events, A2704-12 and A5547-127. J Agric Food Chem. 2011. 59: 13188-94. (通訊作者)

4. Chen L, Guo J, Wang Q, Kai G, Yang LT. Development of the Visual Loop-Mediated Isothermal Amplification Assays for Seven Genetically Modified Maize Events and Their Application in Practical Samples Analysis. J Agric Food Chem. 2011, 59(11): 5914-8. (通訊作者)

5. Zhu X, Chen L, Shen P, Jia J, Zhang D, Yang LT. High sensitive detection of Cry1Ab protein using a quantum dot-based fluorescence-linked immunosorbent assay. J Agric Food Chem. 2011, 59(6):2184-9. (通訊作者)

6. Guo J, Yang LT, Chen L, Morisset D, Li X, Pan L, Zhang D.MPIC: A High-Throughput Analytical Method for Multiple DNA Targets. Anal Chem. 83 (5), 1579–1586 (並列第一作者)

7. Zhi QM, Yang LT, Sun HC. Protective effect of ambroxol against paraquat-induced pulmonary fibrosis in rats. Intern Med. 2011, 50(18):1879-87.

8. Zhi Q, Sun H, Qian X, Yang LT. Edaravone, a novel antidote against lung injury and pulmonary fibrosis induced by paraquat? Int Immunopharmacol. 2011, (1):96-102.

9. Jiang LX, Yang LT, Rao J, Guo JC, Wang S, Liu J, Lee SH, Zhang DB. Development and In-house Validation of the Event-specific Qualitative and Quantitative PCR Detection Methods for Genetically Modified Cotton MON15985. J Sci Food Agric. 2010; 90: 402-8 (並列第一作者)

10. Wang C, Jiang L, Rao J, Liu Y, Yang LT, Zhang D. Evaluation of four genes in rice for their suitability as endogenous reference standards in quantitative PCR. J Agric Food Chem. 2010, 58(22):11543-7.. (通訊作者)

11. Xiaoyan Guan, Jinchao Guo, Ping Shen, Yang LT, Dabing Zhang. Visual and Rapid Detection of Two Genetically Modified Soybean Events Using Loop-mediated Isothermal Amplification Method. Food Analytical Methods. 2010, 3, 313-320. （通訊作者）

12. Shen K, Li X, Wang S, Pan Y, Shi Z, Sun Y, Yang LT. Establishment and in-house validation of simplex and duplex PCR methods for event-specific detection of maize SYN-E3272-5 using a new reference molecule. J AOAC Int. 2010 Mar-Apr;93(2):663-75. (通訊作者)

13. Liu D, Shen J, Yang LT, Zhang D. Evaluation of the impacts of different nuclear DNA content in the hull, endosperm, and embryo of rice seeds on GM rice quantification. J Agric Food Chem. 2010 Apr 28;58(8):4582-7. (通訊作者)

14. Papazova N, Zhang D, Gruden K, Vojvoda J, Yang LT, Buh Gasparic M, Blejec A, Fouilloux S, De Loose M, Taverniers I. Evaluation of the reliability of maize reference assays for GMO quantification. Anal Bioanal Chem. 2010 Mar;396(6):2189-201.

15. Huifeng Shen, Bingjun Qian, Yang LT, Wanqi Liang, Weiwei Chen, Zhenhua Liu and Dabing Zhang. Estimation of the homoplasmy degree for transplastomic tobacco using quantitative real-time PCR. European Food Research and Technology, 2010, 143-150.

16. Shen H, Qian B, Chen W, Liu Z, Yang LT, Zhang D, Liang W. Immunogenicity of recombinant F4 (K88) fimbrial adhesin FaeG expressed in tobacco chloroplast. Acta Biochim Biophys Sin (Shanghai). 2010 Aug;42(8):558-67.

17. Shu Wang, Xiang Li, Yang LT, Kailin Shen, Dabing Zhang. Development and in-house validation of a reference molecule pMIR604 for simplex and duplex event-specific identification and quantification of GM maize MIR604, 2009, Euro Food Res Tech. 230:239–248 （通訊作者）

18. Li X, Yang LT, Zhang J, Wang S, Shen K, Pan L, Zhang D. Simplex and duplex polymerase chain reaction analysis of Herculex RW (59122) maize based on one reference molecule including separated fragments of 5' integration site and endogenous gene. J AOAC Int. 2009 Sep-Oct;92(5):1472-83. (通訊作者)

19. Liu J, Guo J, Zhang H, Li N, Yang LT, Zhang D. Development and in-house validation of the event-specific polymerase chain reaction detection methods for genetically modified soybean MON89788 based on the cloned integration flanking sequence. J Agric Food Chem. 2009 Nov 25;57(22):10524-30. (通訊作者)

20. Guo J, Yang LT, Liu X, Zhang H, Qian B, Zhang D. Applicability of the chymopapain gene used as endogenous reference gene for transgenic huanong no. 1 papaya detection. J Agric Food Chem. 2009 Aug 12;57(15):6502-9. (通訊作者)

21. Guo J, Yang LT, Liu X, Guan X, Jiang L, Zhang D. Characterization of the exogenous insert and development of event-specific PCR detection methods for genetically modified Huanong No. 1 papaya. J Agric Food Chem. 2009 Aug 26;57(16):7205-12. (通訊作者)

22. Jiang L, Yang LT, Zhang H, Guo J, Mazzara M, Van den Eede G, Zhang D. International collaborative study of the endogenous reference gene, sucrose phosphate synthase (SPS), used for qualitative and quantitative analysis of genetically modified rice. J Agric Food Chem. 2009 May 13;57(9):3525-32. (並列第一作者)

23. Zhang HB, Yang LT, Guo JC, Li XH, Feng YM, Zhang DB. Simultaneous detection of Listeria monocytogenes, Staphylococcus aureus, Salmonella enterica and Escherichia coli O157:H7 in food samples using multiplex PCR method. J Food Safety. 2009. 29, 348–363. (並列第一作者)

24. Zhang H, Yang LT, Guo J, Li X, Jiang L, Zhang D. Development of one novel multiple-target plasmid for duplex quantitative PCR analysis of roundup ready soybean. J Agric Food Chem. 2008 Jul 23;56(14):5514-20. Epub 2008 Jun 21. (並列第一作者)

25. Yang LT, Liang W, Jiang L, Li W, Cao W, Wilson ZA, Zhang D. A novel universal real-time PCR system using the attached universal duplex probes for quantitative analysis of nucleic acids. BMC Mol Biol. 2008 Jun 4;9:54.

26. Dong W, Yang LT, Shen K, Kim B, Kleter GA, Marvin HJ, Guo R, Liang W, Zhang D. GMDD: a database of GMO detection methods. BMC Bioinformatics. 2008 Jun 4;9:260. (並列第一作者)

27. Yang LT, Zhang H, Guo J, Pan L, Zhang D. International collaborative study of the endogenous reference gene LAT52 used for qualitative and quantitative analyses of genetically modified tomato. J Agric Food Chem. 2008 May 28;56(10):3438-43. Epub 2008 Apr 29.

28. Yang LT, Guo J, Zhang H, Liu J, Zhang D. Qualitative and quantitative event-specific PCR detection methods for oxy-235 canola based on the 3' integration flanking sequence. J Agric Food Chem. 2008 Mar 26;56(6):1804-9. Epub 2008 Feb 26.

29. Yang LT, Guo J, Pan A, Zhang H, Zhang K, Wang Z, Zhang D. Event-specific quantitative detection of nine genetically modified maizes using one novel standard reference molecule. J Agric Food Chem. 2007 Jan 10;55(1):15-24.

30. Pan L, Zhang S, Yang LT, Broll H, Tian F, Zhang D. Interlaboratory trial validation of an event-specific qualitative polymerase chain reaction-based detection method for genetically modified RT73 rapeseed. J AOAC Int. 2007 Nov-Dec;90(6):1639-46.

31. Yang LT, Pan A, Zhang H, Guo J, Yin C, Zhang D. Event-specific qualitative and quantitative polymerase chain reaction analysis for genetically modified canola T45. J Agric Food Chem. 2006 Dec 27;54(26):9735-40.

32. Pan AH, Yang LT, Xu SC, Yin CS, Zhang KW, Wang ZY, Zhang DB. Event-specific qualitative and quantitative PCR detection of MON863 maize based upon the 3’-transgene integration sequence. J Cereal Sci. 2006, 43: 250-257. (並列第一作者)

33. Zhao ZH, Yang LT (Co-first author), Ai XJ, Zhang DB, Zou SX. Influence of Genetically Modified Rice Containing codA Gene on Physiological Metabolism and Genetic Horizontal Transformation in Fed Rats. Chinese Journal of Agricultural Biotechnology. 2006, 3, 65-70. (並列第一作者)

34. Yang LT, Chen JX, Huang C, Liu YH, Jia SR, Pan LW, Zhang DB. Validation of a cotton-specific gene, Sad1, used as an endogenous reference gene in qualitative and real-time quantitative PCR detection of transgenic cottons. Plant Cell Rep. 2005, 24 (4): 237-245.

35. Yang LT, Ding JY, Zhang CM, Jia JW, Weng HB, Liu WX, Zhang DB. Estimating the copy number of transgenes in transformed rice by real-time quantitative PCR. Plant Cell Rep. 2005, 23 (10-11):759-763.

36. Yang LT, Pan A, Zhang KW, Yin CS, Qian BJ, Chen JX, Huang C, Zhang DB. Qualitative and Quantitative PCR Methods for Event-specific Detection of Genetically Modified Cotton Mon1445 and Mon531. Transgenic Research. 2005, 14, 817-831.

37. Yang LT, Pan AH, Jia JW, Ding JY, Chen JX, Cheng H, Zhang CM, Zhang DB. Validation of a tomato-specific gene, LAT52, used as an endogenous reference gene in qualitative and real-time quantitative PCR detection of transgenic tomatoes. J Agric Food Chem. 2005, 53 (2):183-190.

38. Yang LT, Pan AH, Zhang KW, Guo JC, Yin CS, Chen JX, Huang C, Zhang DB. Identification and quantification of three genetically modified insect resistant cotton lines using conventional and TaqMan real-time polymerase chain reaction methods. J Agric Food Chem. 2005, 53 (16):6222-6229.

39. Yang LT, Shen HF, Pan AH, Chen JX, Huang C, Zhang DB. Screening and construct specific detection methods of transgenic Huafan No.1 tomato by conventional and real-time PCR. J Sci Food Agri, 2005, 85, 2159-2166.

40. Yang LT, Xu SC, Pan AH, Yin CS, Zhang KW, Wang ZY, Zhou ZG, Zhang DB. Event-specific qualitative and quantitative PCR detection of genetically modified MON863 maize based on the 5’-transgene integration sequence. J Agric Food Chem. 2005, 53, 9312-9318.

41. Weng HB, Yang LT, Liu ZL, Ding JY, Pan AH, Zhang DB. Novel reference gene, High-mobility-group protein I/Y, used in qualitative and real-time quantitative polymerase chain reaction detection of transgenic rapeseed cultivars. J AOAC Int. 2005, 88 (2):577-84.

42. Ding JY, Jia JW, Yang LT, Weng HB, Zhang CM, Liu WX, Zhang DB. Validation of a rice specific gene, sucrose phosphate synthase, used as the endogenous reference gene for qualitative and real-time quantitative PCR detection of transgenes. J Agric Food Chem, 2004, 52 (11): 3372-3377.

43. Weng HB, Aihu Pan, Yang LT, Chengmei Zhang, Zhili Liu, Dabing Zhang. Estimating Transgene Copy number by Real -time PCR Assay using HMG I/Y as an Endogenous Reference Gene in Transgenic Rapeseed. Plant Mol. Bio. Rep. 2004, 22, 289–300

楊立桃

基本介紹

個人簡歷

研究方向

發表論文

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