梁朋是基因差異顯示技術和蛋白質三聚體化技術的發明人、白介素24(IL-24)及受體的發現者、p53靶基因 Killin,NDRG1,CYFIP2和TIS11D 的發現者，擁有10項已授權的美國專利。北京大學生物系學士，美國伊利諾斯大學生物化學博士，美國哈佛大學醫學院Dana-Farber癌症研究所博士後，美國Vanderbilt大學終身教授。1997年獲北美華人生物學家年會(SCBA)頒發的“科技發明大獎”, 1998年獲德國分子生物學和生物化學協會頒發的“分子分析大獎”。
基本介紹
- 中文名：梁朋
- 國籍：中國
- 民族：漢
- 畢業院校：北京大學，美國伊利諾斯大學，哈佛大學醫學院，Vanderbilt大學
- 主要成就：10項美國專利
- 代表作品：基因差異顯示技術發明人，白介素24(IL-24)及受體的發現者等
- 職稱：教授
- 職務：董事長
人物簡介,職務,簡歷,研究方向,研究內容,從事科研項目,發表論文,所獲榮譽,
人物簡介
梁朋四川大學生命科學學院教授 博導北京大學生物系學士，美國伊利諾斯大學生物化學博士，美國哈佛大學醫學院 Dana-Farber癌症研究所博士後，美國 Vanderbilt大學終身教授。是基因差異顯示技術和蛋白質三聚體化技術的發明人、白介素24(IL-24)及受體的發現者、p53靶基因 Killin，NDRG1，CYFIP2和TIS11D的發現者，擁有10項已授權的美國專利。1997年獲北美華人生物學家年會(SCBA)頒發的“科技發明大獎”，1998年獲德國分子生物學和生物化學協會頒發的“分子分析大獎”。
職務
美國Vanderbilt(范德堡)大學終身教授 (Associate professor)
GenHunter公司 (美國) 創始人及董事長
四川三葉草生物製藥公司創始人及董事長
四川大學生命科學學院教授
成都市第四屆科技顧問團顧問
簡歷
1978.7-1982.8北京大學生物系 學士
1983 CUSBEA中美交換計畫 留美學生
1984.1-1990.1 美國伊利諾斯大學生物化學 博士
1991.4-1995.8 美國哈佛大學醫學院 Dana-Farber癌症研究所 博士後
1995.9-1999.7美國Vanderbilt(范德堡)大學助理教授（Assistant Professsor）
1999.8-2010.12.31 美國Vanderbilt(范德堡)大學終身教授 (Associate Professor)
1992.10-至今 GenHunter公司 (美國) 創始人及董事長
2007.6-至今 四川三葉草生物製藥公司 創始人及董事長
2007.12-至今 國家生命科學與技術人才培養基地（四川三葉草生物製藥有限公司與四川大學生命科學學院共建）主任
2007.9-2010.7 四川大學 客座教授
2007.9-2010.7 四川大學生命科學學院兼職教授
2008.5-至今 成都市第四屆科技顧問團 顧問
2010.8-至今 四川大學 教授
研究方向
癌症及自身免疫性疾病（如牛皮癬及關節炎）等的發病分子基理及治療手段。
研究內容
本實驗室以運用世界上最先進的螢光基因表達差異顯示（FDD）技術為手段，尋找及發現疾病相關的靶基因，用生物化學及細胞生物學的方法對其進行功能性研究。用AP-Tag技術尋找及研究相關的分泌型細胞因子及其細胞表面受體，並運用現代生物邏輯製藥的方法（包括本實驗室發明的蛋白質三聚體化技術），在生物反應器中採用無血清細胞培養技術研發生產新一代癌症及自身免疫性疾病的靶向抗體及可溶性受體蛋白質藥物。
從事科研項目
（1）科技部科技型中小企業技術創新基金項目：《重組人TNF-α可溶性受體及膠原蛋白三聚體融合蛋白藥物(SCB-131)的開發》
（2） 四川省科技支撐計畫項目:《重組人腫瘤壞死因子相關凋亡誘導配體(TRAIL)三聚體融合蛋白(SCB-313)的研究》
（3）成都市科技攻關計畫：《重組人可溶性RANK配體抑制劑三聚體融合蛋白抗骨質疏鬆藥物的研發》
發表論文
在《科學》、《自然生物技術》、《自然方法學》、《自然癌症綜述》、《美國國家科學院院士通報》和《酶學方法》等世界著名雜誌上發表的文獻近70篇，其ISI引用率超過9000次。
(1) Man Qiao, Dan Luo, Yi Kuang, Haiyan Feng, Guangping Luo and Peng Liang. Cell Cycle Specific Distribution of Killin: Evidence for Negative Regulation of both DNA and RNA Synthesis.Cell Cycle, 2015, in Press.
(2)Xinxin Liu, Anliang Huang, Dan Luo, Haipeng Liu, Huzi Han, Yang Xu, Peng Liang.Use of adenylate kinase as a solubility tag for high level expression of T4 DNA ligase in Escherichia coli Protein Expression and Purification. 2015 Feb; 109 :79–84.
(3) Ma Z,Luo D,Huang A,Xu Y,Wang Y,Wei Y,Liang P. pKILLIN: a versatile positive-selection cloning vector based on the toxicity of Killin in Escherichia coli. Gene. 2014 Jul 10; 544(2):228-35.
(4) Cho YJ, Liang P. S-phase-coupled apoptosis in tumor suppression.Cell Mol Life Sci. 2011 Jun;68(11):1883-96.
(5) He M, Liang P. IL-24 transgenic mice: in vivo evidence of overlapping functions for IL-20, IL-22, and IL-24 in the epidermis.J Immunol. 2010 Feb 15;184(4):1793-8.
(6) Cho YJ, Meade JD, Shester BR, Walden JC, Guo Z, Liang P. Linear dynamic range for signal detection in fluorescent differential display. Biotechnol Lett.2010 Nov;32(11):1593-7.
(7) Cho YJ, Meade JD, Shester BR, Walden JC, Guo Z, Liang P. Proof-reading signal accuracy of gene expression by binary differential display.Biotechnol Lett. 2010 Aug;32(8):1039-44.
(8) Meade JD,Cho YJ,Shester BR,Walden JC,Guo Z,Liang P.Automated fluorescent differential display for cancer gene profiling.Methods Mol Biol. 2010;576:99-133.
(9) S. Zhou,J.D. Meade,S. Nathashon,B. Shester,J. Walden,Z. Guo,J.Z. Liang,J. G. Liang and P. Liang.: Genome-Wide Analysis of mRNA Expression by Florescent Differential Display. The Handbook of Plant Functional Genomics. p165-186. 2008. Wiley-Blackwell publisher.
(10) Cho YJ, Liang P. Killin is a p53-regulated nuclear inhibitor of DNA synthesis. Proc Natl Acad Sci U S A. 2008 Apr 8;105(14):5396-401.
(11) Jackson, R., Cho YJ., Stein, S. andLiang, P.: CYFIP2 is a p53 target gene.Cell Cycle. 2007 Jan;6(1):95-103.
(12) Liang, P. Meade, J. and Pardee, A.B: "A protocol for differential display of mRNA expression using either fluorescent or radioactive labeling" Nature Protocols. 2007, Mar 15, 2:457 - 470.
(13) Liang, P.: MAQC papers over the cracks. Nature Biotechnology. 2007 Jan; 25(1): 27-8.
(14) Jackson, R., Cho YJ., andLiang, P.:TIS11D is a Candidate Pro-Apoptotic p53 Target Gene. Cell Cycle. 2006 Dec;5(24):2889-2893.
(15) Liang P.:From differential display to DNA microarrays-a personal account.J Cell Physiol. 2006 Dec;209(3):653-8.
(16) Tan Z, Wang M, Liang P.: Identification by Differential Display of IL-24 Autocrine Loop Activated by Ras Oncogenes. Methods Mol Biol. 2005;317:207-18.
(17) Meade JD, Cho YJ, Fisher JS, Walden JC, Guo Z, Liang P.: Automation of fluorescent differential display with digital readout. Methods Mol Biol. 2005;317:23-58.
(18) Cho YJ, Stein S, Jackson RS, Liang P.:Saturation Screening for p53 Target Genes by Digital Fluorescent Differential Display. Methods Mol Biol. 2005;317:179-92.
(19) Yang S, Liang P.: Global analysis of gene expression by differential display: a mathematical model. Methods Mol Biol. 2005;317:3-22.
(20) Thomas EK, Nakamura M, Wienke D, Isacke CM, Pozzi A, Liang P.: Endo180 binds to the C-terminal region of alpha 1(I) collagen. J Biol Chem. 2005. 280(24):22596-605.
(21) Wang M, Liang P. Interleukin-24 and its receptors. Immunology. 2005. 114(2):166-70.
(22)Yang,S. and Liang P.:Global analysis of gene expression by differential display, a mathematical model. Mol Biotechnol. 2004. 3:197-208.
(23) Wang, M., Tan,Z., Thomas, E.K. and Liang, P.: Conservation of the genomic structure and receptor-mediated signaling between human and rat IL-24. Genes & Immunity. 2004.5:363-70.
(24) Stein,S., Thomas, E.K., Herzog, B., Westfall, M. D., Rocheleau, J.V., Jackson, R., Wang, M. and Liang,P.:NDRG1 is necessary for p53-dependent apoptosis. J.Biol.Chem., 2004. 279(47): 48930-40
(25) Peng Liang & Arthur B. Pardee.: Analysing differential gene expression in cancer. Nature Reviews Cancer 2003. 3:869-876.
(26) Stein S, Cho, Y. Jackson R.S. and Liang P.: Identification of p53 target genes by fluorescent differential display. Cell Mol Life Sci. 2002 Aug;59(8):1274-9.
(27) Stein S, Liang P.: Differential display analysis of gene expression in mammals: a p53 story. Cell Mol Life Sci. 2002 Aug;59(8):1274-9.
(28) Stein J, Liang P.: Differential display technology: a general guide. Cell Mol Life Sci. 2002 Aug;59(8):1235-40. Review.
(29) Liang P.: SAGE Genie: A suite with panoramic view of gene expression. Proc.Natl.Acad.Sci. U.S.A. 2002. 99:11547-11548.
(30) Liang P.: A decade of differential display. Biotechniques. 2002. 33:338-346.
(31) Cho, Y., Prezioso, V. R. And Liang, P.: Systematic analysis of intrisince factors affecting differential display. Biotechniques 2002, 32:1-4.
(32) Wang, M.,Tan,Z., Zhang, R.,Kotenko, S. V. and Liang, P.: Interleukin-24 (Mob-5/ Mda-7) Signals through Two Heterodimeric Receptors, IL-22R1/IL-20R2 and IL-20R1/IL-20R2. J.Biol.Chem. 2002. 277:7341-7347.
(33) Cho, Y., Meade, J., Walden, J., Guo, Z. and Liang, P.: Multi-color Fluorescent Differential Display. Biotechniques 2001, 30:562-572.
(34) Jo, H., Cho, Y., Zhang, H. and Liang P.: Differential display analysis of altered gene expression by ras oncogene. Methods in Enzymol. (Academic Press),2001. 332: 233-244.
(35) Liang, P.:Gene Discovery Using Differential Display. Genetic Engineering News. 2000. 20:37.
(36)Cho, Y. and Liang, P.: Differential display for detecting potential gene candidates. Molecular Genetics of Mental Disorders, Edited by Briley and Sulser. Martin Dunitz Publishers, London, UK.,2000. In press.
(37)Zhang, R., Tan, Z. and Liang, P.: Identification of a novel ligand-receptor pair constitutively activated by ras oncogene. J. Biol. Chem. 2000, 275:24436-24443.
(38) Jo, H., Zhang, R., Zhang, H. McKinsey, T. A. Ballard, D.W. and Liang, P.: NF-kB is required for H-Ras oncogene induced abnormal cell proliferation and tumorigenesis. Oncogene 2000, 19:841-849.
(39)Liang, P. and Pardee, A. B.: Differential Display, a general Protocol Mol Biotechnology. 1998, 10:261-267.
(40) Jo, Hakryul, Zhang, H., Zhang, R. and Liang, P.: Cloning oncogenic Ras regulated genes by differential display. Methods -A Companion to Methods in Enzymology, Academic Press, 1998, 16:365-375.
(41) Liang, P.: Factors Ensuring successful use of differential display. Methods --A Companion to Methods in Enzymology, Academic Press, 1998, 16:361-364.
(42) Sheng, H., Williams, C. S., Shao, J., Liang, P., DuBois, R. N. and Beauchamp, R. D.: Induction of cyclooxygenase-2 by activated Ha-ras oncogene in Rat-1 fibroblasts and the role of mitogen-activated protein kinase pathway. J.Biol.Chem.1998, 273: 22120-22127.
(43) Zha, ng, R., Averboukh, L., Zhu, W., Zhang, H., Jo, H., Dempsey, P.J., Coffey, R., A. B. Pardee and Liang, P.: Identification of rCop-1, a New Member of CCN Gene Family, as a Negative Regulator for Cell Transformation. Mol. Cell. Biol.,1998, 18:6131-6141.
(44) Hu, J., You, S., Li, W., Wang, D., Nagpal, M. L., Mi, Y., Liang, P. and Lin, Tu.: Expression and regulation of Interferon-g-inducible protein 10 gene in Rat Leydig Cells. Endocrinology 1998, 139:3637-3645.
(45) Liang, P., Wang, F., Zhu, W., O'Connell, R. P., and Averboukh, L.: Identification of novel diagnostic markers by differential display. Methods in Molecular Medicine. Humana Press, 1998, p3-13.
(46) Grady, T., Liang, P., Ernst,S.A.and Logsdon, C. D.: Chemokine gene expression in rat pancreatic acinar cells is an early event associated with acute pancreatitis. Gastroenterology 1997, 113:1966-1975.
(47) Liang, P. and Pardee, A.B.: Differential display: A general protocol. Methods in Molecular Biology. Humana Press. 1997, Vol. 85, p3-12.
(48) Zhang, R., Zhang, H., Zhu, W., Coffey, R., A. B. Pardee and Liang, P.: Mob-1, a ras target gene, is over expressed in colorectal cancer. Oncogene 1997, 14:1607-1610.
(49) Averboukh, L., Liang, P., Kantoff, P. W. and Pardee, A.B.: Regulation of S100P expression by androgen. Prostate, 1996, 29:350-355.
(50) Zhu, W. and Liang, P.: Detection and isolation of differentially expressed genes by differential display. Methods in Molecular Biology, 1996, 68:211-220, Boultwood, J. ed. Humana Press
(51) Liang, P.: Analysis of messenger RNA by differential display. A laboratory guide to RNA, 1996, 223-236. Krieg, P.A. ed., Wiley-Liss.
(52) , Wang, F-L., Wang, Y., Wong, W-K., Liu, Y., Addivinola, F. J., Liang, P., Chen, L-B., Kantoff, P. W. and Pardee, A.B.: Two differentially expressed genes in normal human prostate tissue and in carcinoma. Cancer Res. 1996, 56:3634-3637.
(53) Zhang, H., Zhang, R. and Liang, P.: Differential screening of gene expression difference enriched by differential display. Nucleic Acids Res. , 1996, 24:2454-2456.
(54) Hu, E., Liang, P. and Spiegelman, B. M.: AdipoQ is a novel adipose-specific gene dysregulated in obesity. J.Biol Chem. 1996, 271:10697-10703.
(55) Liang, P., Averboukh, L., Zhu, W., Wang, F., Haley, T. and Pardee, A. B.: Molecular characterization of Murine thymidylate kinase gene. Cell Growth & Differentiation 1995, 6:1333-1338.
(56) Trentmann, S.M., van der Knaap, E., Kende, H., Liang, P. and Pardee A.B.: Alternatives to 35S as a Label for the differential display of eukaryotic messenger RNA. Science 1995, 267: 1186-1187.
(57) Rohrwild, M., Alpan, R.S., Liang, P. and Pardee, A.B.: Inosine-containing primers for mRNA differential display. Trend in Genet., 1995,11:300.
(58) Liang, P. and Pardee, AB.: Recent Advances in Differential Display. Current Opinion in Immunol. 1995, 7:274-280.
(59) Liang, P., Bauer, D., Averboukh, L., Warthoe, P. Rohrwild, M., Muller, H., Strauss, M. and Pardee, A.B.: Analysis of altered gene expression by differential display. in Oncogene Techniques, Methods in Enzymol. (Academic Press),1995, 254:304-321.
(60) Liang, P., Zhu, W., Zhang, X., Guo, Z., O’Connell, R., Averboukh, L. Wang, F. and Pardee, A. B.: Differential display using one-base anchored oligo-dT primer. Nucleic Acids Res., 1994, 22:5763-5764.
(61) Liang, P. and Pardee, A.B., : Differ, ential display of mRNA by PCR. in Current Protocols in Molecular Biology (Wiley Interscience), 1994, Unit 15.8.
(62) Liang, P., Averboukh, L. and Pardee, A. B.: Method of differential display. in Methods in Molecular Genetics (Academic Press), 1994, Vol. 5, pp3-16.
(63) Liang, P., Averboukh, L., Zhu, W. and Pardee, A. B.: Ras activation of novel genes: Mob-1 as a model. Proc Natl Acad.Sci U S.A. ,1994, 91:12515-12519.
(64) Barry M. B., Meador, B., Builerback, T., Liang, P., Glaser, M. and Philips, G. N., Jr.: The closed conformation of a highly flexible protein: the structure of E. coli adenylate kinase with bound AMP and AMPPNP. Proteins 1994, 19:183-198.
(65) Russell, M. E., Utans, U., Wallace, A. F., Liang, P., Karnovsky, M. J., Wyner, L. R., Yamshita, Y. and Chi, T.: Upregulation of Gal/GalNAc macrophage lectin in chronically rejecting rat cardiac allografts with arteriosclerosis. J.Clin.Invest.,1994, 94:722-730.
(66) Utans, U., Liang, P., Wyner, L. R., Karnovsky, M. J. and Russell, M. E.: Chronic cardiac rejection: Identification of five upregulated genes in transplanted hearts by differential mRNA display. Proc.Natl.Acad.Sci.U.S.A.1994, 91:6463-6467.
(67) Sager, R., Anisowicz, A., Neveu, M., Liang, P. and Sotiropoulou, G.: Identification by differential display of alpha 6 integrin as a candidate tumor suppressor gene. FASEB J. 1993, 7:964-970.
(68) Liang, P., Averboukh, L. and Pardee, A. B.: Distribution and cloning of eukayotic mRNAs by means of differential display: refinements and optimization. Nucleic Acids Res. 1993, 21:3269 -3275.
(69) Liang, P. Averboukh, L., Keyomarsi, K., Sager, R. and Pardee, A.B.: Differential display and cloning of mRNAs from human breast cancer vs. mammary epithelial cells. Cancer Research 1992, 52:6966-6968.
(70) Liang, P. and Pardee, A. B.: Differential display of eukaryotic mRNA by means of the polymerase chain reaction. Science 1992, 257:967-971.
(71) Liang, P., Phillips Jr., G. N. and Glaser, M.: Assignment of nucleotide binding sites and the mechanism of substrate inhibition of Escherichia coli adenylate kinase. Proteins 1991, 9:28-36.
(72) Liang, P. and Glaser, M.: Efficient cloning of a mutant adenylate-kinase-encoding gene from Escherichia coli. Gene 1990, 80:21-28.
(73) Althoff, S., Zambrowicx, B., Liang, P., Glaser, M. and Phillips Jr., G. N.: Crystalization and preliminary X-ray analysis of Escherichia coli adenylate kinase. J. Mol. Biol. 1988, 199:665-666.
(74) Liang, P. and Glaser, M.: Rapid recovery and Identification of a temperature- sensitive adenylate kinase gene from Escherichia coli. J.Cell Biol. 1988, 107:306a.
編著：
（1）Liang, P.
and Pardee, A.B.: 《Differential display Methods and Protocols》. Methods in Molecular Biology，Vol. 85, Humana Press. 1979
（2）Liang, P.: 《Current progress in differential display methods and applications》. Methods-- A Companion to Methods in Enzymology, Academic Press, Vol. 16. 1998
（3）Liang, P.
Meade, JD and Pardee, A.B.:《Differential Display Methods and Protocols》.2nd Edition.
Methods in Molecular Biology, Vol. 317, Humana Press. 2005.
獲獎：
1997 獲一年一度的北美華人生物學家年會頒發的“科技發明大獎”
1998 獲二年一度的德國分子生物學和生物化學協會頒發的“分子分析大獎”
2009 四川省第一批“百人計畫”入選者
2009 國務院僑辦“重點華僑華人創業團隊”獲得者
2009 國務院僑辦頒布的第二屆百名華僑華人專業人士“傑出創業獎”
專利：
（A）基因差異顯示 (Differential Display) 技術發明（四項美國專利）
1993-11-16，Arthur Pardee,Liang Peng；Identifying, isolating and cloning messenger RNAs；USA；5, 262, 311
1997-2-4，Liang Peng，Arthur Pardee；Methods of differential display of exposed mRNA by RT/PCR；USA；5, 599, 672
1997-9-9，Arthur Pardee,Liang Peng；Methods of Comparing Levels or Amount of mRNAs；USA；5, 665,547
1999-8-12，Arthur Pardee,Liang Peng；System for Comparing level or amounts of mRNA；USA；5,965,409
（B）蛋白質三聚體化（Trimer-Tag）技術發明（三項美國專利）
2007-9-11，Liang Peng；Methods and Compositions For Producing Secreted Trimeric Receptor Analogs And Biologically Active Fusion Proteins；USA；7,268,116
2010-2-23，Liang Peng；Methods and Compositions For Producing Secreted Trimeric Receptor Analogs And Biologically Active Fusion Proteins；USA；7,666,837
2010-4-6，Liang Peng；Methods for Blocking TNF-alpha Activity in Mammals with Trimeric Soluble TNF Receptors；USA；7,691,815
（C）白介素24（IL-24）和白介素24受體（IL-24 receptors）發現（二項美國專利）
2005-6-7，Liang Peng；Human Mob-5 (IL-24) receptors and uses thereof；USA；6,902,930
2007-8-2，Liang Peng；Human Mob-5 (IL-24) receptors and uses thereof；USA；7,276,237
（D）p53靶基因Killin 發現（一項美國專利）
2009-8-8，Liang Peng, Yongjig Cho；Tumor suppressor Killin；USA；7,576,191
所獲榮譽
2022年，榮評2021四川十大新經濟領軍人物。
