基本介紹
- 中文名：易犁
- 畢業院校：美國密西根大學
- 學位/學歷：博士
- 職業：教師
- 專業方向：生物化學
- 任職院校：湖北大學
人物經歷,社會兼職,榮譽獎項,研究方向,學術成果,
人物經歷
·2015 -，湖北大學生命科學學院教授，博士生導師
·2010 -2015，美國德州大學奧斯汀分校& Clayton研究基金會，博士後，副研究員（Research Associate)，研究員（Research Fellow)，(導師：Prof. George Georgiou & Prof. Brent L. Iverson–美國工程學院院士、美國醫學院院士、美國科學促進協會院士)
·2010，美國密西根大學安娜堡分校，生物化學博士學位(導師：Prof. Stephen W. Ragsdale ­–美國科學促進協會院士、美國微生物協會院士)
·2003，華中農業大學，微生物學碩士學位(導師：周啟教授，周俊初教授，胡傳炯教授)
·2000年，華中農業大學，微生物學學士學位
社會兼職
湖北省生物工程學會理事，生物催化技術湖北省工程實驗室副主任，中國抗生素雜誌編委會委員
榮譽獎項
曾獲美國密西根大學Anthony and Lillian Lu Award
研究方向
分子酶工程（蛋白酶/抗體）、蛋白表達和高通量技術、系統生物學、生物感測
學術成果
已獲得美國授權專利1項、歐盟專利申請1項、加拿大專利申請1項、中國專利申請9項，發表包括PNAS, NAR, JBC, ACS Chem Biol在內的高水平SCI論文30餘篇。
1)Protease/Antibody Engineering for Industrial and Therapeutic Applications:focusing on protease engineering for its applications in industry and medicine (acute autoimmune diseases), scFV and Fab engineering of anti-TNF-α and anti-MMPs antibodies for therapeutics (Yeast surface/Phage display technologies, Structure modeling, Directed evolution,High-throughput screening and sequencing, Biocatalysis, Mass Spectrometry, Enzyme kinetics, Machine Learning, etc.)
2)Protein Folding & Secretion Mechanisms in Eukaryotic Cells:exploring the protein folding and secretion mechanisms in eukaryotic cells, in-depth analysis of protein interactions and modifications in yeast cells, and ER disorder associated human diseases (Systematic biology, Biochemistry, PPI, High-throughput sequencing, Bioinformatics, etc.)
3)Proteins/Chemicals Production in Microbial Systems:developing efficient microbial cell factories (S. cerevisiae, P. pastoris, E. coli, B. subtilis, etc) using systems biology, synthetic biology, and metabolic engineering for proteins and chemicals production (Strain engineering, Protein folding, Secretion, and Cell surface immobilization, etc.)
主持科研項目情況：
1)國家重點研發計畫課題（科技部，2018YFA090091-03），高靈敏環境持久性有毒污染物感知與識別生物系統，2019-2024，150萬，子課題主持人
2)2018年國家自然科學基金（科技部，國家自然科學基金委，31870057）：釀酒酵母內質網滯留信號介導的蛋白滯留效應研究，2019-2022，59萬，主持人
3)國家重點研發計畫課題（2018YFD0500200-03），畜禽廢棄物昆蟲高效轉化機制與調控研究，2018-2020，28萬，子課題主持人
4)2018年針對湖泊水樣及周邊土壤重金屬吸附微生物快速分離、鑑定技術開發，（橫向課題，安徽錦域生物科技有限公司），2018-2019，10萬，主持人；
6)2017年PK15抑制圓環病毒複製相關基因敲除細胞系構建，（橫向課題，浙江美保龍生物技術有限公司），2017-2020，30萬，主持人；
7)2016年國家自然科學基金（國家自然科學基金委，31540068）：酵母內質網內蛋白滯留效應及蛋白酶介導的翻譯後修飾研究，16萬，主持人;
9)2016年湖北省百人計畫資助基金，（湖北省組織部，湖北大學），100萬，主持人；
10)2015年生物資源綠色轉化湖北省協同創新中心人才引進課題資助（湖北省科技廳）：針對具有工業醫用效應酶的分子改造及特性研究，400萬，主持人;
選擇發表論文：
1)Mei, M., Li, J., Wang, S., Lee, B., Iverson B. L., Zhang, G., Ge, X.,* andYi, L.*,"Functional Fab yeast surface display of Adalimumab and its variants against TNF-α through a bi-directional promoter strategy",Front. Bioeng. Biotechnol.,2019
2)Fan, X., Li, X., Zhou, Y., Mei, M., Peng, W., Jiang, Z., Yang, S., Iverson B. L., Zhang, G., * andYi, L.*,"Quantitative analysis of substrate specificities of the 3C protease of human rhinovirus and the exploration of its substrate recognition mechanisms",ACS Chem. Biol., 2019
3)Zheng, Y., Han, J., Wang, B., Hu, X., Li, R., Shen, W., Ma, X., Ma, L.,Yi, L.*,Yang, S.*, and Peng, W.*,"Characterization and repurposing of the endogenous Type I-F CRISPR-Cas system ofZymomonasmobilisfor genome engineering",Nucleic Acids Res., 2019
4)Yang, Y., Shen, W., Li, R., Huang, J., Xiao, Y., Wei, H., Chou, Y., Zhang, M., Himmel, M. E., Chen, S.,Yi, L.,Ma, L., and Yang, S.*, “Application ofZymomonasmobilisin biotechnology based on tools from the system biology era”,Biotechnol.Biofuels, 2019 Mar 14;12:52
5)Lu, Z., Yang, S., Yuan, X., Shi, Y., Ou, Y., Jiang, S.,Yi, L.,and Zhang, G.*, "CRISPR-assisted multi-dimensional regulation for fine-tuning gene expression inBacillus subtilis", 2019,Nucleic Acids Res.,2019 Apr 23;47(7):e40.
6)Mei, M., Zhai, C., Li, X. Z., Zhou, Y., Peng, W. F., Ma, L., Wang, Q. H., Iverson B. L., Zhang, G. M. *, andYi, L.* “Characterization of aromatic residue-controlled protein retention in the endoplasmic reticulum ofSaccharomyces cerevisiae”,J. Biol. Chem.,2017, Dec 15; 292(50):20707-20719
7)Mei, M., Zhou, Y., Peng W. F., Yu, C., Ma, L. X., Zhang, G. M.*, andYi, L.* “Application of Modified Yeast Surface Display Technologies for Non-Antibody Protein Engineering”,Microbiol. Res.,2017 Mar;196:118-128
8)Li, Q.,Yi, L.,Hoi, K. H., Marek, P., Georgiou, G. *, and Iverson, B. L.* “Profiling Protease Specificity: Combining Yeast ER Sequestration Screening (YESS) with Next Generation Sequencing”,ACS Chem. Biol.,2017 Feb 17;12(2):510-518.(#: co-first author.)
- Song, H. T.,Gao, Y., Yang, Y. M., Xiao, W. J., Liu, S. H., Xia, W. C., Liu, Z. L.,Yi, L., and Jiang, Z. B.* “Synergistic effect of cellulase and xylanase during hydrolysis of natural lignocellulosic substrates”,Bioresour. Technol.,2016, 219:710-5
- Yi, L.*,Li, Q., Taft, J. M., Gebhard, M. C., Iverson, B. L. *, and Georgiou, G. * “Yeast Endoplasmic Reticulum Sequestration Screening for the Engineering of Proteases from Libraries Expressed in Yeast”,Methods Mol. Biol.2015, 1319:81-93.
- Li, Q.,Yi, L.,and Marek, P. “Antibodies and Their Multivalent Constructs for Cancer Therapy”,Protein Pept. Lett.2014, 21(10):1017-24
- Yi, L.,Gebhard, M. C., Li, Q., Taft, J. M., Georgiou, G., and Iverson, B. L. “Engineering of TEV protease variants by yeast ER sequestration screening (YESS) of combinatiorial libraries”,PNAS, 2013, 110(18):7229-34.
- Ragsdale, S. W.,Yi, L.,et al. “Redox, Haem and CO in Enzymatic Catalysis and Regulation”,Biochem. Soc. Trans.,2012, 40(3):501-7.
- Ragsdale, S. W., andYi, L.“Thiol/Disulfide Redox Switches in The Regulation of Heme Binding to Proteins”,Antioxid. Redox Signal.,2011, 14(6):1039-1047.
- Yi, L.,Morgan, J. T., and Ragsdale, S. W. “Identification Of a Thiol/Disulfide Redox Switch in The Human BK Channel That Controls Its Affinity For Heme And CO”,J. Biol. Chem.,2010, 285(26):20117-20127.
- Yi, L.,Jenkins, P. M., Leichert, L. I., Jakob, U., Martens, J. R., and Ragsdale, S. W. “Heme Regulatory Motifs in Heme Oxygenase-2 Form a Thiol/Disulfide Redox Switch That Responds to The Cellular Redox State”J. Biol. Chem.,2009, 284, 20556-20561.
- Bianchetti, C. M.,Yi, L.,Ragsdale, S. W., and Philips, G. N., Jr."Comparison Of Apo- And Heme-Bound Crystal Structures Of a Truncated Human Heme Oxygenase-2",J. Biol. Chem.,2007, 282, 37624-37631.
- Yi, L.,and Ragsdale, S. W. "Evidence That The Heme Regulatory Motifs in Heme Oxygenase-2 Serve As a Thiol/Disulfide Redox Switch Regulating Heme Binding",J. Biol. Chem.,2007, 282, 20156-21067.
