封文海

封文海，男，博士，中國農業大學微生物學與免疫學系教授、博士生導師。

基本介紹

• 中文名：封文海
• 畢業院校：美國North Carolina state University
• 學位/學歷：博士
• 專業方向：分子病毒學與免疫學
• 任職院校：中國農業大學

人物經歷

1982年9月--1987年7月北京農業大學動物醫學院 學士

1987年7月--1990年7月北京農業大學動物醫學院 碩士（傳染病）

1990年7月--1993年7月 北京農業大學動物醫學院 助教

1993年8月--1995年12月 美國Iowa State University 碩士（免疫學）

1996年1月--1999年12月美國North Carolina state University 博士（免疫學）

2000年1月--2003年7月美國University of North Carolina 醫學院博士後

2003年8月--2007年3月，美國University of North Carolina 醫學院 Senior Scientist

2007年3月--(原北京農業大學)特聘教授

社會兼職

中國工程院院刊農業學部分刊 FASE 編委

中國獸醫雜誌編委

中國微生物學會病毒學專業委員會委員

Virologica Sinica Editor

主講課程

《免疫學》、《分子病毒學》

研究方向

主要從事病毒和免疫方面研究。研究領域 : 1) 對PRRSV及EBV病毒的不同蛋白質進行分析和研究，從而進一步了解其在病毒感染、複製以及致病過程中所起的作用；2）對PRRSV及EBV病毒的逃避和免疫識別進行研究，以制定出更有效的措施來防制病毒性疾病。

學術成果

發表論文

近年來封文海教授以通訊作者發表的 PRRSV 研究論文（帶*的為通訊作者）：

Liu Y, Du Y, Wang H, Du L, andWen-hai Feng*. 2017. Porcine reproductive and respiratory syndrome virus (PRRSV) up-regulates IL-8 expression through TAK-1JNKAP-1 pathways. Virology. 506:64-72

2.Liu F, Du Y, andWen-hai Feng*. 2017. New perspective of host microRNAs in the control of PRRSV infection. Vet Microbiology. S0378-1135(16)30612-5.

Yu Z, Huang C, Zhang Q, and Wen-hai Feng*. 2016. Porcine reproductive and respiratory syndrome virus (PRRSV) induces IL-12p40 production through JNK-AP-1 and NF-κB signaling pathways. Virus Res. 225:73-81

Du L, Liu Y, Du Y, Wang H, Zhang M, Du Y, and Wen-hai Feng*.2016. Porcine reproductive and respiratory syndrome virus (PRRSV) up-regulates IL-15 through PKCβ1-TAK1-NF-κB signaling pathway. Virology 496:166-74.

Huang C, Du Y, Yu Z, Zhang Q, Liu Y, Tang J, Shi J, and Wen-hai Feng*. 2016. Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus Nsp4 Cleaves VISA to Impair Antiviral Responses Mediated by RIG-I-like Receptors. Scientific Reports 6:28497

Zhang Q, Huang C, Yang Q, Gao L, Liu HC, Tang J, and Wen-hai Feng*. 2016. MicroRNA-30c Modulates Type I IFN Responses To Facilitate Porcine Reproductive and Respiratory Syndrome Virus Infection by Targeting JAK1. Journal of Immunology 196(5):2272-82.

Gao L, Wang L, Huang C, Yang L, Guo XK, Yu Z, Liu Y, Yang P, and Wen-hai Feng*. 2015. HP-PRRSV is attenuated by de-optimization of codon pair bias in its RNA-dependent RNA polymerase nsp9 gene. Virology485:135-44.

Yang Q, Zhang Q, Tang J, and Wen-hai Feng*. 2015. Lipid rafts both in cellular membrane and viral envelope are critical for PRRSV efficient infection. Virology 484:170-180.

Huang C, Zhang Q, and Wen-hai Feng*. 2014. Regulation and evasion of antiviral immune responses by porcine reproductive and respiratorysyndrome virus.Virus Res. 202:101-11.

Wang L, Hou J, Gao L, Guo XK, Yu Z, Zhu Y, Liu Y, Tang J, Zhang H, and Wen-hai Feng*. 2014. Attenuation of highly pathogenic porcine reproductive and respiratory syndrome virus by inserting an additional transcription unit. Vaccine 32(43):5740-8.

Huang C, Zhang Q, Guo XK, Yu ZB, Xu AT, Tang J, and Wen-hai Feng*. 2014. Porcine Reproductive and Respiratory Syndrome Virus Nonstructural Protein 4 Antagonizes IFNbeta Expression by Targeting NEMO. Journal of virology88(18): 10934-10945.

Zhao H, Guo XK, Bi Y, Zhu Y, and Wen-hai Feng*. 2014. PKCσ is required for porcine reproductive and respiratory syndrome virus replication. Virology 468-470:96-103.

Chen XX, Quan R, Guo XK, Gao L, Shi J, and Wen-hai Feng*. 2014. Up-regulation of pro-inflammatory factors by HP-PRRSV infection in microglia: implications for HP-PRRSV neuropathogenesis. Vet Microbiol. 170(1-2):48-57.

Zhang Q, Guo XK, Gao L, Huang C, Li N, Jia X, Liu W, and Wen-hai Feng*. 2014. MicroRNA-23 inhibits PRRSV replication by directly targeting PRRSV RNA and possibly by upregulating type I interferons. Virology 450-451:182-95.

Zhao H, Wang Y, Ma Z, Wang Y, and Wen-hai Feng*. 2014. Recombinant Kluyveromyces lactis expressing highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) GP5 elicits mucosal and cell-mediated immune responses in mice. J Vet Sci. 15(2):199-208.

Bi Y, Guo XK, Zhao H, Gao L, Wang L, Tang J, and Wen-hai Feng*. 2014. Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus Induces Prostaglandin E2 Production through Cyclooxygenase 1, Which Is Dependent on the ERK12-p-CEBP-β Pathway. Journal of Virology 88(5):2810-20.

Ma Z, Zhang W, Wang L, Zhu M, Wang H, and Wen-hai Feng*, Ng TB. 2013. A novel compound from the mushroom Cryptoporus volvatus inhibits porcine reproductive and respiratory syndrome virus (PRRSV) in vitro. PLoS One 8(11):e79333.

Ma Z, Wang Y, Zhao H, Xu AT, Wang Y, Tang J, and Wen-hai Feng*. 2013.Porcine reproductive and respiratory syndrome virus nonstructural protein 4 induces apoptosis dependent on its 3C-like serine protease activity. PLoS One8(7):e69387.

Wang Y, Zhao H, Ma Z, Wang Y, and Wen-hai Feng*. 2013.CTLA4 mediated targeting enhances immunogenicity against PRRSV in a DNA primekilled virus boost strategy. Vet Immunol Immunopathol. 154(3-4):121-8.

Gao L, Guo XK, Wang L, Zhang Q, Li N, Chen XX, Wang Y, and Wen-hai Feng*. 2013. MicroRNA 181 suppresses porcine reproductive and respiratory syndrome virus (PRRSV) infection by targeting PRRSV receptor CD163. Journal of Virology 87(15):8808-12.

Gao L, Zhang W, Sun Y, Yang Q, Ren J, Liu J, Wang H, and Wen-hai Feng*. 2013. Cryptoporus volvatus extract inhibits porcine reproductive and respiratory syndrome virus (PRRSV) in vitro and in vivo. PLoS One. 8(5):e63767.

Yang Q, Gao L, Si J, Sun Y, Liu J, Cao L, and Wen-hai Feng*. 2013. Inhibition of porcine reproductive and respiratory syndrome virus replication by flavaspidic acid AB. Antiviral Res. 97(1):66-73..

Guo XK, Zhang Q, Gao L, Li N, Chen XX, and Wen-hai Feng*. 2013. Increasing expression of microRNA 181 inhibits porcine reproductive and respiratory syndrome virus replication and has implications for controlling virus infection. Journal of Virology 87(2):1159-71.

Wang L, Hou J, Zhang H, and Wen-hai Feng*. 2012.Complete genome sequence of a novel highly pathogenic porcine reproductive and respiratory syndrome virus variant. Journal of Virology 86(23):13121.

Hou J, Wang L, Quan R, Fu Y, Zhang H, and Wen-hai Feng*. 2012. Induction of interleukin-10 is dependent on p38 mitogen-activated protein kinase pathway in macrophages infected with porcine reproductive and respiratory syndrome virus. Virol J. 2012 Aug 21;9:165.

Fu Y, Quan R, Zhang H, Hou J, Tang J, and Wen-hai Feng*. 2012. Porcine reproductive and respiratory syndrome virus induces interleukin-15 through the NF-κB signaling pathway. Journal of Virology 86(14):7625-36.

Hou J, Wang L, He W, Zhang H, and Wen-hai Feng*. 2012Highly pathogenic porcine reproductive and respiratory syndrome virus impairs LPS- and poly(I:C)-stimulated tumor necrosis factor-alpha release by inhibiting ERK signaling pathway. Virus Res. 167(1):106-11.

Wang L, Zhang H, Suo X, Zheng S, and Wen-hai Feng*. 2011. Increase of CD163 but not sialoadhesin on cultured peripheral blood monocytes is coordinated with enhanced susceptibility to porcine reproductive and respiratory syndrome virus infection. Vet Immunol Immunopathol. 141(3-4):209-20.

Quan, R., Y. Fu, W. He, and Wen-hai Feng*. 2012. Cloning and characterization of the porcine IL-10 promoter. Vet Immunol Immunopathol. 146:277-282.

Wen-hai Feng, Tompkins, M.B., Xu, J.S., Zhang, H.X., McCaw, M.B., 2003. Analysis of constitutive cytokine expression by pigs infected in-utero with porcine reproductive and respiratory syndrome virus. Vet. Immunol. Immunopathol. 94(1-2):35-45.

Wen-hai Feng, Tompkins, M, Xu, jin-shen, Brown, T, Laster, S, Zhang, He-xiao, and McCaw, M.B.2002. Thymocyte and peripheral blood T lymphocyte subpopulation changes in piglets following in-uteroinfection with porcine reproductive and respiratory syndrome virus. Virology 302 (2): 363-372.

Wen-hai Feng, S. M. Laster, M. Tompkins, T. Brown, J.-S. Xu, C. Altier, W. Gomez, D. Benfield, and M. B. McCaw. 2001. In Utero Infection by Porcine Reproductive and Respiratory Syndrome Virus Is Sufficient To Increase Susceptibility of Piglets to Challenge by Streptococcus suis Type II. Journal of Virology 75:4889-4895.

EBV相關文章：

Yinping Du, Jieshi Yu, Li Du, JunTang, and Wen-Hai Feng*. 2016. Cordycepin enhances Epstein–Barr virus lytic infection and Epstein–Barr virus-positive tumor treatment efficacy by doxorubicin Epstein–Barr virus-positive tumor treatment efficacy by doxorubicin. Cancer letters, 376:240–248.

Wen-hai Feng,and Shannon C. Kenney. 2007. ZEB positively regulates the lytic-switch BZLF1 gene promoter of Epstein-Barr virus in AGS cells. Journal of Virology, 81: 10113-22.

Jones RJ, Seaman WT, Feng Wen-hai, Tsao SW, Man C, Delecluse HJ, and Kenney SC. 2007. Roles of lytic viral infection and IL-6 in early versus late passage lymphoblastoid cell lines and EBV-associated lymphoproliferative disease. International Journal of Cancer, 121(6):1274-81。

Bhende, PM, Dickerson, S., Feng, Wen-hai, and Kenney SC. 2007. The plasma cell differentiation factor, X-box-binding protein 1 (XBP-1), activates lytic Epstein-Barr virus gene expression in combination with Protein Kinase D (PKD). Journal of Virology, 81(14): 7363-70.

Wen-hai Feng,and Shannon C. Kenney. 2006. Valproic acid enhances chemoagents to treat EBV-positive tumors. Cancer Research, 66 (17) 8762-8768.

Bruce F. Israel, Margaret Gulley, Sandra Elmore, Silvano Ferrini, Wen-hai Feng, and Shannon C. Kenney. 2005. Anti-CD70 antibodies: a potential treatment for EBV-positive CD70-expressing lymphomas. Molecular Cancer Therapeutic, 4(12) 2037-2044.

Gregory K. Hong, Margaret L. Gulley, Wen-hai Feng, Henri-Jacques Delecluse, Elizabeth Holley-Guthrie, and Shannon C. Kenney. 2005. Epstein-Barr Virus Lytic Infection Contributes to Lymphoproliferative Disease in a SCID Mouse Model. Journal of Virology, 79: 13993-14003.

Gregory K. Hong, Henri-Jacques Delecluse, Henri Gruffat, Thomas E. Morrison, Wen-hai Feng, and Shannon C. Kenney. 2004. The BRRF1 early gene of Epstein-Barr virus encodes a transcription factor that enhances induction of lytic infection by BRLF1 Journal of Virology, 78:4983-4992.

Wen-hai Feng, Jeffrey I. Cohen, Henri-Jacques Delecluse, and Shannon C. Kenney. 2004. Methotrexate induces reactivation of latent Epstein-Barr virus (EBV): a potential contributor to methotrexate-associated lymphomas. Journal of the National Cancer Institute, 96(22):1691-1702.

Wen-hai Feng, Gregory Hong, Henri-Jacques Delecluse, and Shannon C. Kenney. Lytic induction therapy for Epstein-Barr virus-positive B-cell lymphomas. 2004. Journal of Virology, 78:1893-1902.

Wen-hai Feng, Bruce Israel, Nancy Raab-Traub, Pierre Busson, and Shannon C. Kenney. 2002. Chemotherapy induces lytic Epstein-Barr virus (EBV) replication and confers ganciclovir susceptibility to EBV-positive epithelial cell tumors. Cancer Research, 62: 1920-1926.

Wen-hai Feng, Eva Westphal, Amy Mauser, Nancy Raab-Traub, Margaret L. Gulley, Pierre Busson, and Shannon C. Kenney. 2002. Use of Adenovirus vectors expressing the Epstein-Barr virus (EBV) immediate-early proteins (BZLF1 or BRLF1) to treat Nasopharyngeal Carcinomas in vivo. Journal of Virology, 76:10951-10959.

Eva M. Westphal, William Blackstock, Wen-hai Feng, Bruce Israel, and Shannon C. Kenney. 2000. Activation of Lytic Epstein-Barr Virus (EBV) Infection by Radiation and Sodium Butyrate in Vitro and in Vivo: A Potential Method for Treating EBV-positive Malignancies. Cancer Research, 60: 5781-5788.

承擔項目

microRNAs調控豬繁殖與呼吸綜合徵病毒感染和細胞嗜性的分子機制研究國家自然科學基金重點項目（2017-01-01至2021-12-31）334萬 主持

高致病性豬繁殖與呼吸綜合徵病毒（HP-PRRSV）引起高熱的分子機制研究國家自然科學基金面上項目（2016-01-01至2019-12-31）76.2萬 主持

PRRSV干預抗病毒細胞因子表達和功能的分子機制北京市自然科學基金重點項目（2015-01-01至2018-12-31）65 萬 主持

動物重要基因克隆及功能驗證(2016-2018) 轉基因生物新品種培育專項（2016-01-01至2018-12-31）2273.1萬 參加

豬重要疫病免疫防控新技術研究國家重點研發計畫（2017年7月至2020年12月） 58萬參加

發明專利

重組豬繁殖與呼吸綜合徵病毒及其製備方法與套用（專利號201310018715.5）授權單位：中華人民共和國國家知識產權局 第一發明人：封文海

重組PRRS病毒HV-nsp29及其套用（專利號201310394862.2） 授權單位：中華人民共和國國家知識產權局 第一發明人：封文海

重組PRRS病毒HV-nsp9及其套用（專利號201310394833.6）授權單位：中華人民共和國國家知識產權局 第一發明人：封文海