寡脫氧胸苷酸可用於研究不同偶聯模式的興奮性胺基酸受體所介導洲爪蟾卵母非細胞跨膜電流的相互影響。
基本介紹
- 中文名：寡脫氧胸苷酸
- 外文名：Deoxidization thymidylic acid
- 性質：科學
- 類別：生物
簡介,相關資料,
簡介
目的研究不同偶聯模式的興奮性胺基酸受體所介導洲爪蟾卵母非細胞跨膜電流的相互影響。方法用異硫氰酸胍-酚-氯仿法從成年大鼠腦中提取總RNA,以寡脫氧胸苷酸纖維素親和層析法分離出mRNA並注射入非洲爪蟾卵母細胞使表達,通過全細胞雙電極電壓鉗位法,分別以M-膽鹼受體Carb,谷氨酸離子型受體激動劑kainate（KA）,代謝Ⅰ型受體激動劑quisqualate（QA）及代謝Ⅲ型受體激動劑L-phosphoserine（L-SOP）兩兩同時灌流有受體表達的非洲爪蟾卵母細胞。結果3種偶聯模式的受體激動後產生的膜電流具有交叉脫敏現象。結論在多種受體共存的細胞中,受體之間可能存在著廣泛的相互作用。
相關資料
[1]Vickery-RM,Morris-SH,BindmanLJ.Metabotropicglutamatereceptorsareinvolvedinlong-termpotentiationinisolatedslicesofratmedialfrontalcortex[J].J-Neurophysiol,1997,78(6):3039
[2]袁芳王天佑.代謝型谷氨酸受體在腦缺血缺氧損傷中的作用[J].基礎醫學與臨床,:.
[3]Pin-JP,Duvoisin-R.Themetabotropicglutamatereceptors:structureandfunctions[J].Neuropharmacology,1995,34(1):1
[4]Guatteo-E,Mercuri-NB,BernardiG,etal.GroupImetabotropicglutamatereceptorsmediateaninwardcurrentinratsubstantianigradopamineneuronsthatisindependentfromcalciummobilization[J].J-Neurophysiol,1999,82(4):1974
[5]KrupnickJG,BenovicJL.TheroleofreceptorkinasesandarrestinsinGprotein-coupledreceptorregulation[J].Annu.Rev.Pharmacol.Toxicol,1998,38:289
[6]陳中玉陳厚昌蔣毅萍.非洲爪蟾卵母細胞Ca^2+誘發鈣振盪的途徑,5-羥色胺受體和毒覃鹼型膽鹼受體介導非洲爪蟾卵母細胞鈣電流的特徵[C]..第一軍醫大學研究生論文集[C].,1998.6..
[7]陳厚昌HESS,CP.菸鹼受體在非洲爪蟾卵母細胞的表達及特徵[J].中國藥理學與毒理學雜誌,:.
[8]DascalN,GilloB,LassY.Roleofcalciummobilizationinmediationofacetylcholine-evokedchloridecurrentsinXenopuslaevisoocytes[J].JPhysiol,1985,366:299
[9]陳厚昌蔣毅萍陳中玉.電壓鉗位法檢測細胞內鈣振盪[J].第一軍醫大學學報,1999,19(6):504.
[10]ParkerI,MilediR.ChangesinintracellularcalciumandinmembranecurrentsevokedbyinjectionofinositoltrisphosphateintoXenopusoocytes[J].ProceedingofRoyalSociety,1986,288(1252):307
[11]Sanna-E,Dildy-Mayfield-JE,HarrisRA.Ethanolinhibitsthefunctionof5-hydmxytryptaminetypelcandmuscarinicM1Gprotein-linkedreceptorsinXenopusoocytesexpressingbrainmRNA:roleofproteinkinaseC[J].Mol-Pharmacol,1994,45(5):1004
[12]Gereau-RW4^th,Heinemann-SF.RoleofproteinkinaseCphos-phorylationinrapiddesensitizationofmetabotropicglutamatereceptor5[J].Neuron,1998,20(1):143
[13]Dildy-Mayfield-JE,Harris-RA.ActivationofproteinkinaseCinhibitskainate-inducedcurrentsinoocytesexpressingglutamatereceptorsubunits[J].J-Neurochem,1994,62(4):1639
[14]Swope-SL,Moss-SI,RaymondLA,etal.Regulationofligand-gatedionchannelsbyproteinphosphorylafon[J].Adv-Second-Messenger-Phosphoprotein-Res,1999,33:49
[15]Herrem-I,Miras-Portugal-MT,Sanchez-prietoJ.Rapiddesensitizationofthemetabotropicglutamatereceptorthatfacilitatesglutamatereleaseinratcerebrocorticalnerveterminals[J].Eur-J-Neurosci,1994,6(1):115
