《基於毛細管電泳的蛋白質糖基化譜高靈敏度方法研究》是張新祥為項目負責人，北京大學為依託單位的面上項目。
基本介紹
- 中文名：基於毛細管電泳的蛋白質糖基化譜高靈敏度方法研究
- 項目類別 ：面上項目
- 項目負責人：張新祥
- 依託單位 ：北京大學
科研成果,項目摘要,
科研成果
序號 | 標題 | 類型 | 作者 |
|---|---|---|---|
1 | 固相萃取-高效液相色譜法測定紫杉醇脂質體的包封率 | 期刊論文 | 張一鳴|劉靖新|周穎琳|張新祥| |
2 | Hydrazino-s-triazine based labelling reagents for highly sensitive glycan analysis via liquid chromatography-electrospray mass spectrometry | 期刊論文 | Deng, Yan|Liu, Jing-Xin|Zhou, Ying-Lin|Zhang, Xin-Xiang| |
3 | Differential detection of Rhizoma coptidis by capillary electrophoresis electrospray ionization mass spectrometry with a nanospray interface | 期刊論文 | 張新祥| |
4 | Double-layer poly(vinyl alcohol)-coated capillary for highly sensitive and stable capillary electrophoresis and capillary electrophoresis with mass spectrometry glycan analysis | 期刊論文 | Zhao, Ming-Zhe|Liu, Jing-Xin|Zhou, Ying-Lin|Zhang, Xin-Xiang| |
5 | A smart tailor-made G-clip reporter for sensitive detection of G-triplet-containing sequences | 期刊論文 | Zhang, Yi-Wei|Zhang, Fang-Ting|Zhou, Ying-Lin|Zhang, Xin-Xiang| |
6 | A G-quadruplex based platform for label-free monitoring of DNA reaction kinetics | 期刊論文 | Zhang, Fang-Ting|Zhao, Ming-Zhe|Zhou, Ying-Lin|Zhang, Xin-Xiang| |
7 | G-quadruplex based two-stage isothermal exponential amplification reaction for label-free DNA colorimetric detection | 期刊論文 | Zhang, De-Wen|Tie, Cai|Zhou, Ying-Lin|Zhang, Xin-Xiang| |
8 | Ultrasensitive determination of 5-methylcytosine and 5-hydroxymethylcytosine in genomic DNA by sheathless interfaced capillary electrophoresis-mass spectrometry | 期刊論文 | Nie, Ji|Chen, Hong-Xu|Zhou, Ying-Lin|Zhang, Xin-Xiang| |
9 | Layered double hydroxide-hemin nanocomposite as mimetic peroxidase and its application in sensing | 期刊論文 | Zhou, Ying-Lin|Ma, Yu-Rong|Qi, Li-Min|Zhang, Xin-Xiang| |
10 | A facilely synthesized amino-functionalized metal-organic framework for highly specific and efficient enrichment of glycopeptides | 期刊論文 | Zhao, Qiang|Zhou, Ying-Lin|Liu, Hu-Wei|Zhang, Xin-Xiang| |
11 | A label-free DNA hairpin biosensor for colorimetric detection of target with suitable functional DNA partners | 期刊論文 | Zhang, De-Wen|Tie, Cai|Zhou, Ying-Lin|Zhang, Xin-Xiang| |
12 | DNA cross-triggered cascading self-amplification artificial biochemical circuit | 期刊論文 | Xie, Wen Jun|Cai, Liang-Yuan|Zhou, Ying-Lin|Zhang, Xin-Xiang| |
13 | A cupric ion triggered DNA diode based on a tandem linkage-cleavage reaction | 期刊論文 | Zhang, Xiao-Hui|Zhang, De-Wen|Zhou, Ying-Lin|Zhang, Xin-Xiang| |
14 | Deuterated hydrazino-s-triazine as highly-efficient labelling reagent for glycan relative quantification analysis using electrospray ionization mass spectrometry | 期刊論文 | Deng, Yan|Zhang, Fang-Ting|Zhou, Ying-Lin|Zhang, Xin-Xiang| |
15 | Portable, Easy-to-Operate, and Antifouling Microcapsule Array Chips Fabricated by 3D Ice Printing for Visual Target Detection | 期刊論文 | Huang, Dong|Zhou, Ying-Lin|Li, Zhi-Hong|Zhang, Xin-Xiang| |
16 | Methylene Blue as a G-Quadruplex Binding Probe for Label-Free Homogeneous Electrochemical Biosensing | 期刊論文 | Zhang, De-Wen|Chen, Ji-Tao|Zhou, Ying-Lin|Zhang, Xin-Xiang| |
17 | Novel Homogeneous Label-Free Electrochemical Aptasensor Based on Functional DNA Hairpin for Target Detection | 期刊論文 | Zhang, Fang-Ting|Xu, Li|Zhou, Ying-Lin|Zhang, Xin-Xiang| |
18 | Reporter-triggered isothermal exponential amplification strategy in ultrasensitive homogeneous label-free electrochemical nucleic acid biosensing | 期刊論文 | Zhang, Fang-Ting|Yuan, Fang|Zhou, Ying-Lin|Zhang, Xin-Xiang| |
19 | Hybridization chain reaction-based fluorescence immunoassay using DNA intercalating dye for signal readout | 期刊論文 | Zhang, Xiao-hui|Zhao, Ming-Zhe|Zhou, Ying-Lin|Zhang, Xin-Xiang| |
項目摘要
多糖是自然界含量最豐富的生物聚合物, 廣泛表達於微生物、植物和動物中, 並以糖基化的方式形成糖蛋白參與所有蛋白質分子的結構穩態和功能調節，主要包括細胞信號傳遞、基因表達、免疫回響以及蛋白質摺疊等方面，蛋白質糖基化的變異是生物體發生變異的主要途徑之一，準確高靈敏度測定蛋白質糖基化變化是理解和調節生命行為的基礎工作。本課題擬以建立蛋白質糖基化特徵譜高靈敏度分析方法為目標，通過新型免疫識別體系-新衍生方法-同位素標記衍生試劑相結合，開發出基於毛細管電泳-雷射誘導螢光高靈敏度檢測及毛細管電泳-無鞘液接口質譜鑑定的糖基化變異檢測的新方法，為蛋白質糖基化的快速鑑定特別是疾病前期預警建立有效工具。
