劉成輝，男，博士，陝西師範大學化學化工學院教授、博士生導師。
基本介紹
- 中文名：劉成輝
- 畢業院校：清華大學
- 學位/學歷：博士
- 職業：教師
- 專業方向：生化分析及分子診斷
- 職務：陝西師範大學化學化工學院博士生導師
- 任職院校：陝西師範大學化學化工學院
- 性別：男
個人經歷,研究方向,學術成果,榮譽獎勵,
個人經歷
2009年7月於清華大學化學系獲理學博士遙汽厚學位。現為陝師大化學化工學院教授，分析化學和化學生物學專業博士生導師。
現擔任Wiley出版社ChemistrySelect期刊編委。
研究方向
研究方向：生化分析及分子診斷。
主要研究興趣為：（1）生化分析與分子診斷；（2）功甩洪煮禁能材料表界面感測；（3）單分子與單敬騙鴉想顆粒分定詢騙燥循再析。
學術成果
2016年獲得國家基金委“優秀青年科學基金”資助；先後主主笑持國家自然科學基金等省部級以上項洪甩獄目9項。作為通訊作者或第一作者在Angew. Chem. Int. Ed.,Chem. Sci.,Anal. Chem.,Chem. Commun.等發表論文50餘篇。
30.Gaoting Wang, Weimin Tian, Xiaoling Liu*, Wei Ren, Chenghui Liu*. A new CRISPR-derived microRNA sensing mechanism based on Cas12a self-powered and rolling circle transcription (RCT)-unleashed real-time crRNA recruiting.Anal. Chem.,2020, DOI: 10.1021/acs.analchem.0c00680.
29.Hui Tian, Wenhan Zhao, Xiaolong Liu, Chenghui Liu*, Niancai Peng*. An integrated single microbead-arrayed μ-fluidic platform for the automated detection of multiplexed biomarkers.ACS Sens.,2020, 5, 798-806.
28.Zhenhao Zhang, Dandan Yang, Weimin Tian, Yan Qi, Wei Ren, Zhengping Li, Chenghui Liu*. Facile Clamp-Assisted Ligation Strategy for Direct Discrimination and Background-Free Quantification of Site-Specific 5‑Formylcytosine.Anal. Chem.,2020, 92, 3477-3482.
27.Wenjiao Fan, Wei Ren*, Liping Zhu, Chenghui Liu*.On-bead enzyme-catalyzed signal amplification for the high-sensitive detection of disease biomarkers.Methods in Enzymology,2020, 630, 179-197. (Invited Book Chapter)
26.Fu Chang, Yueying Sun, Dandan Yang, Wenjuan Yang, Yuanyuan Sun, Chenghui Liu*, Zhengping Li*. Specific Detection of RNA Mutation at Single-Base Resolution by Coupling Isothermal Exponential Amplification Reaction (EXPAR) with Chimeric DNA Probe-Aided Precise RNA Disconnection at the Mutation Site.Chem. Commun.,2019, 55, 6934-6937.
25.Weimin Tian, Pengjie Li, Wenli He, Chenghui Liu*, Zhengping Li*. Rolling Circle Extension-Actuated Loop-Mediated Isothermal Amplification (RCA-LAMP) for Ultrasensitive Detection of MicroRNAs.Biosens. Bioelectron.,2019, 128, 17-22.
24.Ya Li, Dailu Jia, Wei Ren*, Feng Shi, Chenghui Liu*. A Versatile Photoinduced Electron Transfer-Based Upconversion Fluorescent Biosensing Platform for the Detection of Disease Biomarkers and Nerve Agent.Adv. Funct. Mater.,2019, 29, 1903191.
23.Xiaohui Lu, Wenjiao Fan, Qinya Feng, Yan Qi, Chenghui Liu*, Zhengping Li. A Versatile Dynamic Light Scattering Strategy for the Sensitive Detection of Plant MicroRNAs Based-on Click Chemistry-Amplified Aggregation of Gold Nanoparticles.Chem. Eur. J.,2019, 25, 1701-1705.(Very Important Paper)
22.Yan Qi, Xiaohui Lu, Qinya Feng, Wenjiao Fan, Chenghui Liu*, Zhengping Li*. An Enzyme-Free MicroRNA Assay Based-on Fluorescence Counting of Click Chemical Ligation-Illuminated Magnetic Nanoparticles with Total Internal Reflection Fluorescence Microscopy.ACS Sens.,2018, 3, 2667-2674.
21.Yuanyuan Sun, Hui Tian, Chenghui Liu*, Dandan Yang, Zhengping Li*. A Clamp-Based One-Step Droplet Digital Reverse Transcription PCR (ddRT-PCR) for Precise Quantitation of Messenger RNA Mutation in Single Cells.ACS Sens.,2018, 3, 1795-1801.
20.Liping Zhu, Desheng Chen, Xiaohui Lu, Yan Qi, Pan He, Chenghui Liu,* Zhengping Li. An Ultrasensitive Flow Cytometric Immunoassay Based on Bead Surface-Initiated Template-Free DNA Extension.Chem. Sci.,2018, 9, 6605-6613.(Inside Front Cover, Hot Article of Chem Sci)
19.Wenjiao Fan, Yan Qi, Liying Qiu, Pan He, Chenghui Liu,* Zhengping Li. Click Chemical Ligation-Initiated On-Bead DNA Polymerization for the Sensitive Flow Cytometric Detection of 3´-Terminal 2´-O-Methylated Plant MicroRNA.Anal. Chem.,2018, 90, 5390-5397.
18.Yueying Sun, Yuanyuan Sun, Weimin Tian, Chenghui Liu,* Kejian Gao, Zhengping Li.*A novel restriction endonuclease GlaI for rapid and highly sensitive detection of DNA methylation coupled with isothermal exponential amplification reaction.Chem. Sci.,2018, 9, 1344-1351.
17.Desheng Chen, Xiaobo Zhang, Liping Zhu, Chenghui Liu,* Zhengping Li.* Single Microbead-Anchored Fluorescent Immunoassay (SMFIA): A Facile and Versatile Platform Allowing Simultaneous Detection of Multiple Antigens.Chem. Asian J.,2017, 12, 2894-2898.(Featured as Front Cover)
16.Yuanyuan Sun, Hui Tian, Chenghui Liu,* Yueying Sun, Zhengping Li.* One-step detection of microRNA with high sensitivity and specificity via target-triggered loop-mediated isothermal amplification (TT-LAMP).Chem. Commun.,2017, 53, 11040-11043.
15.Liying Qiu, Yuecheng Zhang, Chenghui Liu,* Zhengping Li.* A Versatile Size-Coded Flow Cytometric Bead Assay for Simultaneous Detection of Multiple MicroRNAs Coupled with a Two-Step Cascading Signal Amplification.Chem. Commun.,2017, 53, 2926-2929.
14.Xiangdong Wang, Hui Wang, Chenghui Liu,* Honghong Wang, Zhengping Li.* A three-way junction structure-based isothermal exponential amplification strategy for sensitive detection of 3’-terminal 2’-O-methylated plant microRNA.Chem. Commun.,2017, 53, 1124-1127.
13.Hui Tian, Yuanyuan Sun, Chenghui Liu,* Xinrui Duan, Wei Tang, Zhengping Li.* Precise Quantitation of MicroRNA in a Single Cell with Droplet Digital PCR Based on Ligation Reaction.Anal. Chem.,2016, 88, 11384−11389.
12.Junting Li, Qi Zhao, Feng Shi, Chenghui Liu,* Yanli Tang.* NIR-Mediated Nanohybrids of Upconversion Nanophosphors and Fluorescent Conjugated Polymers for High-Efficiency Antibacterial Performance Based on Fluorescence Resonance Energy Transfer.Adv. Healthcare Mater.,2016, 5, 2967-2971.(Highlighted by MaterialsViewsChina)
11.Chao Jiang, Ya Li, Chenghui Liu,* Liying Qiu, Zhengping Li. A General and Versatile Fluorescence Turn-On Assay for Detecting the Activity of Protein Tyrosine Kinases Based on Phosphorylation-Inhibited Tyrosyl Oxidation.Chem. Commun.,2016, 52, 12570-12573.(Featured as Front Cover)
10.Honghong Wang, Hui Wang, Chenghui Liu,* Xinrui Duan, Zhengping Li.* Ultrasensitive Detection of Telomerase Activity in a Single Cell Using Stem-loop Primer-mediated Exponential Amplification (SPEA) with Near Zero Nonspecific Signal.Chem. Sci.,2016, 7, 4945-4950.
9.Xiaohui Lu, Peipei Wang, Yucong Wang,* Chenghui Liu,* Zhengping Li. A Versatile Dual-Emission Fluorescent Microhybrid Enabling Visual Detection of Glucose and Other Oxidases-Based Biocatalytic Systems.Adv. Mater. Technol.,2016, 1, 1600024.(Inside Front Cover, Highlighted by MaterialsViewsChina)
8.XiaoboZhang, Chenghui Liu,* Honghong Wang, Hui Wang, Zhengping Li.* Rare Earth Ion Mediated Fluorescence Accumulation on a Single Microbead: An Ultrasensitive Strategy for the Detection of Protein Kinase Activity at the Single-Cell Level.Angew. Chem. Int. Ed.,2015, 54, 15186-15190.
7.Xiaobo Zhang, Chenghui Liu,* Lingbo Sun, Xinrui Duan, Zhengping Li.* Lab on a single microbead: an ultrasensitive detection strategy enabling microRNA analysis at the single-molecule level.Chem. Sci.,2015, 6, 6213-6218.(Highlighted by ChemistryWorld and Chemical Science Blog, Hot Article)
6.Haoxian Zhou, Hui Wang, Chenghui Liu,* Honghong Wang, Xinrui Duan, Zhengping Li.* Ultrasensitive genotyping with target-specifically generated circular DNA templates and RNA FRET probes.Chem. Commun.,2015, 51, 11556-11559.(Featured as Front Cover, Hot Article of ChemComm)
5.Yuecheng Zhang, Chenghui Liu,* Sujuan Sun, Yanli Tang, Zhengping Li.* Phosphorylation-Induced Hybridization Chain Reaction on Beads: An Ultrasensitive Flow Cytometric Assay for the Detection of T4 Polynucleotide Kinase Activity.Chem. Commun.,2015, 51, 5832-5835.(Featured as Inside Front Cover)
4.Chenghui Liu,* Fangfang Wang, Yucong Wang, Zhengping Li.* A versatile fluorescence turn-on assay for highly sensitive detection of tyrosine phosphatase activity.Chem. Commun.,2014, 50, 13983-13986.
3.Pengpo Zhang, Jiangyan Zhang, Chengli Wang, Chenghui Liu,* Hui Wang, Zhengping Li.* Highly sensitive and specific multiplexed microRNA quantification using size-coded ligation chain reaction (LCR).Anal. Chem.,2014, 86, 1076-1082.
2.Chenghui Liu,* Lijuan Chang, Honghong Wang, Jie Bai, Wei Ren, Zhengping Li. Upconversion Nanophosphor: An Efficient Phosphopeptides-Recognizing Matrix and Luminescence Resonance Energy Transfer Donor for Robust Detection of Protein Kinase Activity.Anal. Chem.,2014, 86, 6095-6102.
1.Fangfang Wang, Chenghui Liu,* Yunlong Fan, Yucong Wang, Zhengping Li.* Robust detection of tyrosine phosphatase activity by coupling chymotrypsin-assisted selective peptide cleavage and a graphene oxide-based fluorescent platform.Chem. Commun.,2014, 50, 8161-8163.
榮譽獎勵
2015年度“陝西省青年科技新星”。
26.Fu Chang, Yueying Sun, Dandan Yang, Wenjuan Yang, Yuanyuan Sun, Chenghui Liu*, Zhengping Li*. Specific Detection of RNA Mutation at Single-Base Resolution by Coupling Isothermal Exponential Amplification Reaction (EXPAR) with Chimeric DNA Probe-Aided Precise RNA Disconnection at the Mutation Site.Chem. Commun.,2019, 55, 6934-6937.
25.Weimin Tian, Pengjie Li, Wenli He, Chenghui Liu*, Zhengping Li*. Rolling Circle Extension-Actuated Loop-Mediated Isothermal Amplification (RCA-LAMP) for Ultrasensitive Detection of MicroRNAs.Biosens. Bioelectron.,2019, 128, 17-22.
24.Ya Li, Dailu Jia, Wei Ren*, Feng Shi, Chenghui Liu*. A Versatile Photoinduced Electron Transfer-Based Upconversion Fluorescent Biosensing Platform for the Detection of Disease Biomarkers and Nerve Agent.Adv. Funct. Mater.,2019, 29, 1903191.
23.Xiaohui Lu, Wenjiao Fan, Qinya Feng, Yan Qi, Chenghui Liu*, Zhengping Li. A Versatile Dynamic Light Scattering Strategy for the Sensitive Detection of Plant MicroRNAs Based-on Click Chemistry-Amplified Aggregation of Gold Nanoparticles.Chem. Eur. J.,2019, 25, 1701-1705.(Very Important Paper)
22.Yan Qi, Xiaohui Lu, Qinya Feng, Wenjiao Fan, Chenghui Liu*, Zhengping Li*. An Enzyme-Free MicroRNA Assay Based-on Fluorescence Counting of Click Chemical Ligation-Illuminated Magnetic Nanoparticles with Total Internal Reflection Fluorescence Microscopy.ACS Sens.,2018, 3, 2667-2674.
21.Yuanyuan Sun, Hui Tian, Chenghui Liu*, Dandan Yang, Zhengping Li*. A Clamp-Based One-Step Droplet Digital Reverse Transcription PCR (ddRT-PCR) for Precise Quantitation of Messenger RNA Mutation in Single Cells.ACS Sens.,2018, 3, 1795-1801.
20.Liping Zhu, Desheng Chen, Xiaohui Lu, Yan Qi, Pan He, Chenghui Liu,* Zhengping Li. An Ultrasensitive Flow Cytometric Immunoassay Based on Bead Surface-Initiated Template-Free DNA Extension.Chem. Sci.,2018, 9, 6605-6613.(Inside Front Cover, Hot Article of Chem Sci)
19.Wenjiao Fan, Yan Qi, Liying Qiu, Pan He, Chenghui Liu,* Zhengping Li. Click Chemical Ligation-Initiated On-Bead DNA Polymerization for the Sensitive Flow Cytometric Detection of 3´-Terminal 2´-O-Methylated Plant MicroRNA.Anal. Chem.,2018, 90, 5390-5397.
18.Yueying Sun, Yuanyuan Sun, Weimin Tian, Chenghui Liu,* Kejian Gao, Zhengping Li.*A novel restriction endonuclease GlaI for rapid and highly sensitive detection of DNA methylation coupled with isothermal exponential amplification reaction.Chem. Sci.,2018, 9, 1344-1351.
17.Desheng Chen, Xiaobo Zhang, Liping Zhu, Chenghui Liu,* Zhengping Li.* Single Microbead-Anchored Fluorescent Immunoassay (SMFIA): A Facile and Versatile Platform Allowing Simultaneous Detection of Multiple Antigens.Chem. Asian J.,2017, 12, 2894-2898.(Featured as Front Cover)
16.Yuanyuan Sun, Hui Tian, Chenghui Liu,* Yueying Sun, Zhengping Li.* One-step detection of microRNA with high sensitivity and specificity via target-triggered loop-mediated isothermal amplification (TT-LAMP).Chem. Commun.,2017, 53, 11040-11043.
15.Liying Qiu, Yuecheng Zhang, Chenghui Liu,* Zhengping Li.* A Versatile Size-Coded Flow Cytometric Bead Assay for Simultaneous Detection of Multiple MicroRNAs Coupled with a Two-Step Cascading Signal Amplification.Chem. Commun.,2017, 53, 2926-2929.
14.Xiangdong Wang, Hui Wang, Chenghui Liu,* Honghong Wang, Zhengping Li.* A three-way junction structure-based isothermal exponential amplification strategy for sensitive detection of 3’-terminal 2’-O-methylated plant microRNA.Chem. Commun.,2017, 53, 1124-1127.
13.Hui Tian, Yuanyuan Sun, Chenghui Liu,* Xinrui Duan, Wei Tang, Zhengping Li.* Precise Quantitation of MicroRNA in a Single Cell with Droplet Digital PCR Based on Ligation Reaction.Anal. Chem.,2016, 88, 11384−11389.
12.Junting Li, Qi Zhao, Feng Shi, Chenghui Liu,* Yanli Tang.* NIR-Mediated Nanohybrids of Upconversion Nanophosphors and Fluorescent Conjugated Polymers for High-Efficiency Antibacterial Performance Based on Fluorescence Resonance Energy Transfer.Adv. Healthcare Mater.,2016, 5, 2967-2971.(Highlighted by MaterialsViewsChina)
11.Chao Jiang, Ya Li, Chenghui Liu,* Liying Qiu, Zhengping Li. A General and Versatile Fluorescence Turn-On Assay for Detecting the Activity of Protein Tyrosine Kinases Based on Phosphorylation-Inhibited Tyrosyl Oxidation.Chem. Commun.,2016, 52, 12570-12573.(Featured as Front Cover)
10.Honghong Wang, Hui Wang, Chenghui Liu,* Xinrui Duan, Zhengping Li.* Ultrasensitive Detection of Telomerase Activity in a Single Cell Using Stem-loop Primer-mediated Exponential Amplification (SPEA) with Near Zero Nonspecific Signal.Chem. Sci.,2016, 7, 4945-4950.
9.Xiaohui Lu, Peipei Wang, Yucong Wang,* Chenghui Liu,* Zhengping Li. A Versatile Dual-Emission Fluorescent Microhybrid Enabling Visual Detection of Glucose and Other Oxidases-Based Biocatalytic Systems.Adv. Mater. Technol.,2016, 1, 1600024.(Inside Front Cover, Highlighted by MaterialsViewsChina)
8.XiaoboZhang, Chenghui Liu,* Honghong Wang, Hui Wang, Zhengping Li.* Rare Earth Ion Mediated Fluorescence Accumulation on a Single Microbead: An Ultrasensitive Strategy for the Detection of Protein Kinase Activity at the Single-Cell Level.Angew. Chem. Int. Ed.,2015, 54, 15186-15190.
7.Xiaobo Zhang, Chenghui Liu,* Lingbo Sun, Xinrui Duan, Zhengping Li.* Lab on a single microbead: an ultrasensitive detection strategy enabling microRNA analysis at the single-molecule level.Chem. Sci.,2015, 6, 6213-6218.(Highlighted by ChemistryWorld and Chemical Science Blog, Hot Article)
6.Haoxian Zhou, Hui Wang, Chenghui Liu,* Honghong Wang, Xinrui Duan, Zhengping Li.* Ultrasensitive genotyping with target-specifically generated circular DNA templates and RNA FRET probes.Chem. Commun.,2015, 51, 11556-11559.(Featured as Front Cover, Hot Article of ChemComm)
5.Yuecheng Zhang, Chenghui Liu,* Sujuan Sun, Yanli Tang, Zhengping Li.* Phosphorylation-Induced Hybridization Chain Reaction on Beads: An Ultrasensitive Flow Cytometric Assay for the Detection of T4 Polynucleotide Kinase Activity.Chem. Commun.,2015, 51, 5832-5835.(Featured as Inside Front Cover)
4.Chenghui Liu,* Fangfang Wang, Yucong Wang, Zhengping Li.* A versatile fluorescence turn-on assay for highly sensitive detection of tyrosine phosphatase activity.Chem. Commun.,2014, 50, 13983-13986.
3.Pengpo Zhang, Jiangyan Zhang, Chengli Wang, Chenghui Liu,* Hui Wang, Zhengping Li.* Highly sensitive and specific multiplexed microRNA quantification using size-coded ligation chain reaction (LCR).Anal. Chem.,2014, 86, 1076-1082.
2.Chenghui Liu,* Lijuan Chang, Honghong Wang, Jie Bai, Wei Ren, Zhengping Li. Upconversion Nanophosphor: An Efficient Phosphopeptides-Recognizing Matrix and Luminescence Resonance Energy Transfer Donor for Robust Detection of Protein Kinase Activity.Anal. Chem.,2014, 86, 6095-6102.
1.Fangfang Wang, Chenghui Liu,* Yunlong Fan, Yucong Wang, Zhengping Li.* Robust detection of tyrosine phosphatase activity by coupling chymotrypsin-assisted selective peptide cleavage and a graphene oxide-based fluorescent platform.Chem. Commun.,2014, 50, 8161-8163.
榮譽獎勵
2015年度“陝西省青年科技新星”。
