中華屈撓桿菌由眉藻 (Calothrix) 培養池中分離得到一株寬度均一, 長度不等 (0.4-0.5 X 4.0-16.0 micrometer 以上) 的屈撓桿菌, 在0.5%胰腖液體培養基中呈長絲狀,長度可超過 60 micrometer. 細胞有時聚集成團。
編號 | AMS-84-02 |
拉丁菌名 | Flexibacter chinenses sp. nov. Li, Li et Wang |
中文菌名 | 中華屈撓桿菌 |
定名人 | 李勤生 黎尚豪 王大耜 |
Nomencla | Li Qinsheng, Li Shanghao and Wang Dasi |
發表文章 | 中華屈撓桿菌的分離和鑑定 |
Article | Isolation and Characterization of Flexibacter chinenses sp. nov. |
作者 | 李勤生 黎尚豪 / 王大耜 |
Author | Li Qinsheng and Li Shanghao / Wang Dasi |
作者單位 | 中國科學院水生生物研究所, 武 漢 430071 / 中國科學院微生物研究所細菌分離學實驗室 , 北京 100080 |
摘要 | 由眉藻 (Calothrix) 培養池中分離得到一株寬度均一, 長度不等 (0.4-0.5 X 4.0-16.0 micrometer 以上) 的屈撓桿菌, 在0.5%胰腖液體培養基中呈長絲狀,長度可超過 60 micrometer. 細胞有時聚集成團. 兼性厭氧. 革蘭氏陰性 , 無內生芽孢及其它形式的休眠細胞. 滑行運動. 能利用 多種糖類作為碳源, 但不分解瓊脂,幾丁質和纖維素. 可 以NO3-, NH4+, 酪蛋白水解物作為唯一氮源. 不液化明膠 . 在胰陳瓊脂上形成粉紅-橙色粘性菌落. 色素光譜測定 最高吸收峰值為484nm(正己烷提取), 在乙醇提取物中為 484nm, 510nm. DNA中6+C含量為34.2克分子%. 對固氮藍 藻營養細胞有溶解能力. 對四環素以外的多種抗菌素均具 有相當強的耐受性.與已知的屈撓桿菌有明顯的差異, 認 為是一新種, 命名為中華屈撓桿菌 (Flexibacter chinenses sp. nov.). 模式株FCA存於中國科學院水生生 物研究所. |
Abstract | Strain FCA was isolated from the Calothrix nursery bed in the Institute of Hydrobiology. It is a flexible rod or filament in shape with uniform width andvarious length (0.4-0.5 by 4.0-16.0 micrometer). When it is grown in the liquid medium the length is usually more than 60.0 micrometer.Sometimes the cells aggregate to form a mass. Motile by gliding, resting stages not known. Gram-negative. The colour of colonies of strain FCA is pink, with a pale iridesence, and turn to orange with ageing. But on the third day the cell mass becomes colourless.The maximum absorption peak of crude extract of pigment (in n-hexane or ethanol) is at 484nm. Facultatively anaerobic; hydrolyzes starch; many sugars are utilized and acid is produced from glucose, malatose, lactose, galactose, sucrose, xylose,anabionose, rafinose and cellobiose, but not from mannitol, fructose and glycerol. Cellulose (filter paper or carboxymethyl cellulose), chitin and agarare not attacked. Casein hydrolysate, tryptone, ammonium or nitrate alone is utilized as a sole N source. Nitrate is reduced to nitrite.Gelatin and tyrosine are not degraded. The DNA G+C content of strain FCA is 34.2 moles %. The initial isolate possesses ability to lyse vegetative cells of Anabaena strain 1105. Growth of strain FCA is inhibited by 0.01% SDS, susceptible to tetracycline 10 u/mL, resistant to 10 ug actinomycin D, 2,000 u polymyxin B or E,1,600 u penicillin G, 60 u streptomycin, 25 ug mitomycin C, 600 u erythromycin, 100 u chloramphenicol, and 200 u neomycin per mL etc. This strain belongs to the genus Flexibacter, but it differs significantly from species previously described. Therefore it is regarded as anew species, for which the name Flexibacter chinenses is proposed. Type culture FCA is deposited in the Institute of Hydrobiology, Academia Sinica, Wuhan. |
關鍵字 | 屈撓桿菌屬, 中華屈撓桿菌, 眉藻 |
Key | |
期刊 | 微生物學報 24(1):7-13, 1984 |
Publication | Acta Microbiologica Sinica (Chinese; English Abstract), 24(1):pp.7-13, 1984. |
分享省市 | 湖北省武漢市 |
Place | Wuhan City, Hubei Province, China |
採集地 | 武昌區東湖風景區由中國科學院水生生物研究所實 驗用眉藻培養池 |
Environment | The bacterium isolated from the Calothrix nursery bed near East Lake Scenic Spot at Wuchang District. |
海撥高度 | |
生態環境 | 亞熱帶季風氣候園林 |
寄主 | 眉藻 |
Habitat | subtropical monsoon climate the Calothrix nursery bed. |
Host | Calothrix |
採集人 | 李勤生 黎尚豪 |
Isolation Person | Li Qinsheng and Li Shanghao |
採集時間 | |
保存單位 | 中國科學院水生生物研究所, 武漢 430071 |
Preservation Unit | Institute of Hydrobiology, The Chinese Academy of Scieces, Wuhan 430071, Hubei Province, China |
描述 | |
等模式 | |
Speciman No. | FCA |
全文 | |
Note | The bacterium is a pathogen for the Calothrix sp. and Anaboena 1105 etc. |
備註 | 該菌是培育眉藻和魚腥藻等的病害. |
